Pescadillo (PES1) (NM_014303) Human Tagged ORF Clone

CAT#: RC207096

PES1 (Myc-DDK-tagged)-Human pescadillo homolog 1, containing BRCT domain (zebrafish) (PES1), transcript variant 1

Product Images

Circular map for RC207096

• 

  Western blot validation of overexpression lysate (Cat# LY415373) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207096 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pescadillo
• Synonyms: NOP7; PES
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207096 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGGCCTTGAGAAGAAGAAGTATGAACGAGGCTCGGCCACCAACTACATCACCCGGAACAAAGCCC
  GGAAGAAGCTCCAGCTGAGCTTGGCTGACTTTAGGCGGCTGTGCATTCTGAAGGGCATTTATCCCCATGA
  ACCCAAACACAAGAAGAAGGTTAACAAGGGTTCTACAGCAGCCCGAACGTTTTACCTTATCAAAGACATC
  AGGTTTCTCCTCCACGAACCCATTGTCAACAAGTTCCGTGAATACAAGGTGTTCGTCCGGAAGCTCCGGA
  AGGCTTATGGGAAGAGCGAGTGGAACACTGTAGAGCGTTTAAAGGACAATAAGCCCAACTACAAACTCGA
  CCACATCATCAAGGAACGGTATCCCACGTTCATCGATGCCCTGCGGGACCTGGACGATGCCCTCTCCATG
  TGCTTCCTGTTTTCCACCTTCCCGCGGACTGGCAAGTGCCACGTGCAGACCATTCAGCTGTGCCGCCGGC
  TCACTGTGGAGTTCATGCACTACATTATCGCTGCCCGTGCCCTGCGCAAGGTCTTCCTGTCCATCAAAGG
  CATTTACTACCAGGCCGAGGTACTGGGGCAGCCCATCGTGTGGATCACTCCCTATGCCTTCTCCCATGAC
  CACCCGACAGACGTGGACTACAGGGTCATGGCCACCTTCACCGAGTTCTACACCACGCTGCTGGGCTTTG
  TCAACTTCCGCCTTTACCAGTTGCTCAACCTCCACTATCCCCCGAAGCTCGAGGGTCAGGCCCAAGCAGA
  GGCAAAGGCCGGTGAGGGCACCTACGCGTTGGACTCCGAGAGTTGTATGGAGAAACTGGCAGCCCTCAGT
  GCCAGCCTGGCCCGCGTGGTGGTGCCTGCCACAGAGGAGGAGGCCGAGGTGGATGAGTTTCCCACCGATG
  GGGAGATGTCAGCGCAGGAGGAAGACCGCAGGAAGGAGCTGGAGGCGCAGGAGAAGCACAAGAAGCTTTT
  TGAGGGCCTGAAGTTCTTCCTGAACCGAGAGGTGCCCCGTGAGGCCCTGGCCTTCATCATCAGGAGTTTT
  GGTGGGGAAGTGTCCTGGGACAAATCTTTGTGCATTGGGGCCACCTATGACGTCACAGACTCCCGCATCA
  CCCATCAGATTGTCGACCGGCCTGGGCAGCAGACCTCAGTCATTGGCAGGTGCTACGTGCAGCCCCAGTG
  GGTGTTTGACTCAGTGAACGCCAGGCTCCTTCTCCCCGTGGCAGAGTACTTCTCTGGGGTGCAGCTGCCC
  CCACACCTTTCACCCTTTGTGACCGAGAAGGAAGGAGATTACGTTCCACCTGAGAAGCTGAAGCTGCTGG
  CTCTGCAGCGGGGAGAGGACCCAGGAAACCTGAATGAGTCAGAAGAGGAGGAGGAAGAGGACGACAACAA
  CGAAGGTGATGGTGATGAAGAGGGAGAAAATGAGGAGGAGGAGGAAGATGCAGAGGCTGGTTCAGAAAAG
  GAGGAAGAGGCCCGGCTGGCAGCCCTGGAAGAGCAGAGGATGGAGGGGAAGAAGCCCAGGGTGATGGCAG
  GCACCTTGAAGCTGGAGGATAAGCAGCGGCTGGCCCAGGAGGAGGAGAGTGAGGCCAAGCGCCTGGCCAT
  TATGATGATGAAGAAGCGGGAGAAGTACCTGTACCAGAAGATCATGTTTGGCAAGAGGCGAAAAATCCGA
  GAGGCCAACAAGCTGGCGGAGAAGCGGAAAGCCCACGATGAGGCGGTGAGGTCTGAGAAGAAGGCCAAGA
  AGGCAAGGCCGGAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207096 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGGLEKKKYERGSATNYITRNKARKKLQLSLADFRRLCILKGIYPHEPKHKKKVNKGSTAARTFYLIKDI
  RFLLHEPIVNKFREYKVFVRKLRKAYGKSEWNTVERLKDNKPNYKLDHIIKERYPTFIDALRDLDDALSM
  CFLFSTFPRTGKCHVQTIQLCRRLTVEFMHYIIAARALRKVFLSIKGIYYQAEVLGQPIVWITPYAFSHD
  HPTDVDYRVMATFTEFYTTLLGFVNFRLYQLLNLHYPPKLEGQAQAEAKAGEGTYALDSESCMEKLAALS
  ASLARVVVPATEEEAEVDEFPTDGEMSAQEEDRRKELEAQEKHKKLFEGLKFFLNREVPREALAFIIRSF
  GGEVSWDKSLCIGATYDVTDSRITHQIVDRPGQQTSVIGRCYVQPQWVFDSVNARLLLPVAEYFSGVQLP
  PHLSPFVTEKEGDYVPPEKLKLLALQRGEDPGNLNESEEEEEEDDNNEGDGDEEGENEEEEEDAEAGSEK
  EEEARLAALEEQRMEGKKPRVMAGTLKLEDKQRLAQEEESEAKRLAIMMMKKREKYLYQKIMFGKRRKIR
  EANKLAEKRKAHDEAVRSEKKAKKARPE
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014303
• ORF Size: 1764 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014303.4
• RefSeq Size: 2312 bp
• RefSeq ORF: 1767 bp
• Locus ID: 23481
• UniProt ID: O00541
• Cytogenetics: 22q12.2
• Domains: BRCT
• MW: 68 kDa
• Gene Summary: This gene encodes a nuclear protein that contains a breast cancer associated gene 1 (BRCA1) C-terminal interaction domain. The encoded protein interacts with BOP1 and WDR12 to form the PeBoW complex, which plays a critical role in cell proliferation via pre-rRNA processing and 60S ribosomal subunit maturation. Expression of this gene may play an important role in breast cancer proliferation and tumorigenicity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the long arm of chromosome 4 and the short arm of chromosome 9. [provided by RefSeq, Aug 2011]