PDP2 (NM_020786) Human Tagged ORF Clone

CAT#: RC207071

PDP2 (Myc-DDK-tagged)-Human pyruvate dehyrogenase phosphatase catalytic subunit 2 (PDP2)

Product Images

Circular map for RC207071

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  Western blot validation of overexpression lysate (Cat# LY402805) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207071 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PDP2 protein (Cat# TP307071). The protein was produced from HEK293T cells transfected with PDP2 cDNA clone (Cat# RC207071) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PDP2
• Synonyms: PDPC 2; PPM2B; PPM2C2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207071 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAAGTACTGTGTCCTACTGGATCTTAAATTCTACAAGGAACAGCATTGCCACATTGCAAGGGGGTA
  GACGCTTATACTCCAGGTATGTCTCAAATAGGAATAAATTAAAATGGAGGCTCTTTTCCCGGGTGCCACC
  CACCCTAAACAGTTCCCCATGTGGTGGCTTTACTCTGTGCAAAGCCTACAGACACACATCAACAGAGGAA
  GATGATTTTCACTTGCAACTCAGCCCTGAGCAGATAAATGAAGTGCTTCGAGCTGGCGAGACAACCCACA
  AGATTCTTGACCTTGAAAGCAGAGTCCCAAATTCAGTGTTGCGGTTTGAGAGCAACCAGCTGGCTGCCAA
  TTCCCCAGTGGAGGACCGGCGAGGTGTAGCCTCCTGCCTGCAAACCAATGGACTGATGTTTGGCATCTTC
  GATGGACATGGTGGTCATGCATGTGCCCAAGCAGTGAGCGAGAGGCTCTTCTACTATGTGGCAGTGTCCC
  TGATGTCCCACCAGACCCTGGAGCACATGGAGGGAGCTATGGAAAGCATGAAACCCTTGCTGCCCATCCT
  GCATTGGCTCAAGCACCCAGGGGACAGTATCTACAAGGATGTCACATCTGTGCATCTTGACCACCTCCGT
  GTCTATTGGCAGGAACTGCTTGATTTGCACATGGAAATGGGACTAAGCATTGAAGAAGCATTAATGTACT
  CCTTCCAGAGACTGGATTCTGACATCTCGCTGGAAATCCAGGCCCCCCTGGAAGATGAGGTGACAAGGAA
  CCTGTCACTCCAGGTTGCTTTCTCTGGGGCAACAGCTTGCATGGCCCATGTTGATGGAATTCACTTGCAC
  GTGGCAAATGCTGGTGACTGCCGAGCCATCCTTGGTGTCCAAGAGGACAATGGCATGTGGTCTTGTCTGC
  CCCTTACACGTGACCACAATGCCTGGAACCAGGCCGAGCTGTCCCGGCTAAAGAGGGAGCACCCTGAGTC
  AGAGGACAGGACGATCATCATGGAGGACAGGCTACTGGGCGTCCTCATCCCCTGCAGGGCCTTTGGGGAT
  GTTCAGCTGAAGTGGAGTAAAGAGTTGCAGCGCAGCATTCTGGAGAGGGGCTTCAATACCGAGGCCCTCA
  ACATTTACCAGTTCACACCCCCACACTACTACACTCCACCCTACCTGACTGCTGAGCCTGAGGTCACATA
  CCACAGGCTGAGGCCCCAGGATAAGTTCCTTGTGCTGGCCTCAGATGGCCTGTGGGACATGCTGAGCAAT
  GAGGACGTGGTAAGGCTGGTGGTGGGGCACCTGGCTGAGGCAGATTGGCACAAGACAGACCTGGCCCAGA
  GACCCGCCAACTTGGGGCTCATGCAGAGCCTGCTGCTGCAGAGGAAAGCCAGCGGGCTCCACGAGGCTGA
  CCAAAATGCAGCCACGCGGCTGATCAGACATGCCATCGGGAACAATGAGTATGGGGAGATGGAGGCAGAG
  CGGCTGGCGGCGATGCTGACATTGCCAGAGGACTTGGCGAGGATGTACAGGGATGATATCACTGTCACTG
  TGGTGTATTTTAACTCAGAATCAATCGGTGCATATTACAAGGGGGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207071 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSTVSYWILNSTRNSIATLQGGRRLYSRYVSNRNKLKWRLFSRVPPTLNSSPCGGFTLCKAYRHTSTEE
  DDFHLQLSPEQINEVLRAGETTHKILDLESRVPNSVLRFESNQLAANSPVEDRRGVASCLQTNGLMFGIF
  DGHGGHACAQAVSERLFYYVAVSLMSHQTLEHMEGAMESMKPLLPILHWLKHPGDSIYKDVTSVHLDHLR
  VYWQELLDLHMEMGLSIEEALMYSFQRLDSDISLEIQAPLEDEVTRNLSLQVAFSGATACMAHVDGIHLH
  VANAGDCRAILGVQEDNGMWSCLPLTRDHNAWNQAELSRLKREHPESEDRTIIMEDRLLGVLIPCRAFGD
  VQLKWSKELQRSILERGFNTEALNIYQFTPPHYYTPPYLTAEPEVTYHRLRPQDKFLVLASDGLWDMLSN
  EDVVRLVVGHLAEADWHKTDLAQRPANLGLMQSLLLQRKASGLHEADQNAATRLIRHAIGNNEYGEMEAE
  RLAAMLTLPEDLARMYRDDITVTVVYFNSESIGAYYKGG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020786
• ORF Size: 1587 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020786.4
• RefSeq Size: 7042 bp
• RefSeq ORF: 1590 bp
• Locus ID: 57546
• UniProt ID: Q9P2J9
• Cytogenetics: 16q22.1
• Protein Families: Druggable Genome, Phosphatase
• MW: 60 kDa
• Gene Summary: This gene is a mitochondrial protein that functions as a phosphatase and is involved in the enzymatic resetting of the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Aug 2016]