Cullin 4B (CUL4B) (NM_003588) Human Tagged ORF Clone

CAT#: RC206935

CUL4B (Myc-DDK-tagged)-Human cullin 4B (CUL4B), transcript variant 1

Product Images

Circular map for RC206935

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CUL4B (Cat# RC206935, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CUL4B(Cat# TA800444). Positive lysates LY418558 (100ug) and LC418558 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY418558) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206935 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CUL4B protein (Cat# TP306935). The protein was produced from HEK293T cells transfected with CUL4B cDNA clone (Cat# RC206935) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cullin 4B
• Synonyms: CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206935 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTCACAGTCATCTGGATCAGGAGATGGGAATGATGATGAGGCTACTACCTCTAAAGACGGTGGTT
  TTTCTTCCCCCAGTCCCTCAGCTGCTGCTGCTGCTCAGGAGGTCAGATCTGCCACTGATGGTAATACCAG
  CACCACTCCGCCCACCTCTGCCAAGAAGAGAAAGTTAAACAGCAGCAGCAGTAGCAGCAGTAACAGTAGT
  AACGAGAGAGAAGACTTTGATTCCACCTCTTCCTCCTCTTCCACTCCTCCTTTACAACCCAGGGATTCGG
  CATCCCCTTCAACCTCGTCCTTCTGCCTGGGGGTTTCAGTGGCTGCTTCCAGCCACGTACCGATACAGAA
  GAAGCTGCGTTTTGAAGACACCCTGGAGTTTGTAGGGTTTGATGCGAAGATGGCTGAGGAATCCTCCTCC
  TCCTCCTCCTCATCTTCACCAACTGCTGCAACATCTCAGCAGCAGCAACTTAAAAATAAGAGTATATTAA
  TCTCTTCTGTGGCTTCGGTGCATCATGCAAACGGCCTAGCCAAATCTTCTACCACCGTCTCTAGCTTTGC
  TAACAGCAAACCTGGCTCTGCTAAGAAGTTAGTGATCAAGAACTTTAAAGATAAGCCTAAATTACCAGAA
  AACTACACAGATGAAACCTGGCAAAAACTGAAAGAAGCAGTGGAAGCTATTCAGAATAGTACTTCAATTA
  AGTACAATTTAGAAGAACTCTACCAGGCTGTAGAAAATCTCTGTTCTTACAAGATTTCTGCAAACTTGTA
  CAAACAGCTGAGACAGATCTGCGAAGATCACATCAAAGCACAGATTCATCAATTCAGAGAGGATTCATTG
  GATAGCGTTCTTTTTTTAAAGAAGATTGATAGATGCTGGCAAAACCATTGCAGACAAATGATCATGATCA
  GGAGCATTTTTTTGTTTCTGGATAGAACTTACGTTCTTCAGAATTCAATGCTACCCTCCATTTGGGACAT
  GGGACTGGAGTTATTTAGGGCTCATATTATAAGTGATCAGAAAGTGCAGAATAAGACAATTGATGGCATT
  CTTCTCTTGATTGAGAGGGAAAGGAATGGTGAAGCAATTGATAGAAGTTTACTTCGAAGCCTTTTAAGCA
  TGCTGTCTGATTTGCAAATTTATCAAGATTCTTTTGAACAACGATTTTTGGAAGAAACTAACCGGCTCTA
  TGCAGCTGAAGGCCAAAAATTAATGCAAGAAAGAGAGGTTCCTGAATATCTACATCATGTTAACAAACGT
  CTAGAAGAAGAAGCAGACAGACTTATTACTTACTTAGATCAGACCACCCAGAAGTCATTAATTGCTACTG
  TAGAAAAACAACTTCTAGGTGAACACTTAACAGCAATTCTTCAGAAAGGTTTAAATAACCTCCTTGATGA
  AAACCGAATTCAAGATTTGTCTCTTCTGTATCAGCTCTTCAGTAGAGTTCGAGGTGGAGTTCAGGTTCTT
  TTGCAGCAGTGGATCGAATATATCAAGGCATTTGGCAGCACTATTGTAATTAATCCTGAAAAAGATAAAA
  CCATGGTTCAAGAATTGCTGGATTTTAAAGATAAGGTTGACCATATAATTGATATCTGCTTTCTGAAGAA
  TGAGAAATTTATCAATGCCATGAAAGAAGCATTTGAAACGTTCATTAACAAAAGACCAAATAAACCAGCT
  GAACTTATAGCTAAGTATGTAGATTCAAAACTTCGTGCAGGCAACAAAGAAGCTACAGATGAAGAACTTG
  AGAAAATGTTGGATAAAATTATGATCATATTTAGATTTATCTATGGCAAGGATGTTTTTGAGGCCTTCTA
  TAAGAAAGATTTAGCCAAGCGCCTGTTAGTCGGAAAGAGTGCATCTGTAGATGCTGAAAAATCAATGCTG
  TCCAAACTTAAACATGAATGCGGAGCTGCTTTCACCAGCAAACTTGAAGGAATGTTTAAAGACATGGAAC
  TTTCTAAAGACATCATGATTCAGTTCAAACAGTATATGCAGAATCAGAATGTTCCGGGAAATATTGAGTT
  AACTGTGAATATCCTGACAATGGGCTATTGGCCGACATATGTGCCTATGGAAGTTCATTTACCACCAGAG
  ATGGTAAAACTTCAGGAGATTTTCAAGACATTTTACCTAGGCAAACATAGTGGCAGGAAACTTCAGTGGC
  AGTCAACCATAGGACACTGTGTGTTAAAAGCAGAATTTAAAGAGGGTAAAAAGGAACTCCAGGTCTCTCT
  TTTTCAAACACTGGTGCTGCTAATGTTTAATGAGGGAGAGGAGTTCAGTTTAGAAGAGATCAAGCAGGCA
  ACTGGAATAGAGGATGGAGAGTTAAGGAGAACACTGCAGTCATTAGCCTGTGGCAAAGCTAGAGTTCTGG
  CGAAAAATCCAAAGGGCAAAGACATTGAAGATGGTGACAAGTTCATTTGTAATGATGATTTCAAACATAA
  ACTTTTCAGGATAAAGATCAATCAAATCCAGATGAAAGAAACGGTTGAAGAACAAGCAAGCACTACAGAA
  AGAGTATTTCAAGACAGACAGTATCAAATTGATGCTGCAATTGTTCGAATTATGAAGATGAGAAAGACAC
  TTAGCCACAATCTCCTTGTTTCAGAAGTGTACAACCAGTTGAAATTTCCAGTAAAGCCTGCTGATCTTAA
  GAAGAGAATAGAATCTTTAATTGACCGGGACTACATGGAAAGAGATAAAGAAAATCCAAACCAGTACAAC
  TATATTGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206935 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMSQSSGSGDGNDDEATTSKDGGFSSPSPSAAAAAQEVRSATDGNTSTTPPTSAKKRKLNSSSSSSSNSS
  NEREDFDSTSSSSSTPPLQPRDSASPSTSSFCLGVSVAASSHVPIQKKLRFEDTLEFVGFDAKMAEESSS
  SSSSSSPTAATSQQQQLKNKSILISSVASVHHANGLAKSSTTVSSFANSKPGSAKKLVIKNFKDKPKLPE
  NYTDETWQKLKEAVEAIQNSTSIKYNLEELYQAVENLCSYKISANLYKQLRQICEDHIKAQIHQFREDSL
  DSVLFLKKIDRCWQNHCRQMIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRAHIISDQKVQNKTIDGI
  LLLIERERNGEAIDRSLLRSLLSMLSDLQIYQDSFEQRFLEETNRLYAAEGQKLMQEREVPEYLHHVNKR
  LEEEADRLITYLDQTTQKSLIATVEKQLLGEHLTAILQKGLNNLLDENRIQDLSLLYQLFSRVRGGVQVL
  LQQWIEYIKAFGSTIVINPEKDKTMVQELLDFKDKVDHIIDICFLKNEKFINAMKEAFETFINKRPNKPA
  ELIAKYVDSKLRAGNKEATDEELEKMLDKIMIIFRFIYGKDVFEAFYKKDLAKRLLVGKSASVDAEKSML
  SKLKHECGAAFTSKLEGMFKDMELSKDIMIQFKQYMQNQNVPGNIELTVNILTMGYWPTYVPMEVHLPPE
  MVKLQEIFKTFYLGKHSGRKLQWQSTIGHCVLKAEFKEGKKELQVSLFQTLVLLMFNEGEEFSLEEIKQA
  TGIEDGELRRTLQSLACGKARVLAKNPKGKDIEDGDKFICNDDFKHKLFRIKINQIQMKETVEEQASTTE
  RVFQDRQYQIDAAIVRIMKMRKTLSHNLLVSEVYNQLKFPVKPADLKKRIESLIDRDYMERDKENPNQYN
  YIA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003588
• ORF Size: 2739 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003588.2, NP_003579.2
• RefSeq Size: 5365 bp
• RefSeq ORF: 2742 bp
• Locus ID: 8450
• UniProt ID: Q13620
• Cytogenetics: Xq24
• Domains: CULLIN
• Protein Pathways: Nucleotide excision repair, Ubiquitin mediated proteolysis
• MW: 104 kDa
• Gene Summary: This gene is a member of the cullin family. The encoded protein forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]