CYP27C1 (NM_001001665) Human Tagged ORF Clone

CAT#: RC206930

CYP27C1 (Myc-DDK-tagged)-Human cytochrome P450, family 27, subfamily C, polypeptide 1 (CYP27C1)

Product Images

Circular map for RC206930

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  Western blot validation of overexpression lysate (Cat# LY424236) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206930 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CYP27C1 protein (Cat# TP306930). The protein was produced from HEK293T cells transfected with CYP27C1 cDNA clone (Cat# RC206930) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CYP27C1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206930 representing NM_001001665
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAAGCGTATTGAGACAAAGAATTCTGAAACCGAAAGATGTGGCCATTTATTCTGGAGAAGTCAACC
  AAGTTATTGCTGACTTAATTAAAAGAATCTACCTCCTCAGGAGCCAGGCAGAAGATGGAGAAACCGTGAC
  CAATGTCAATGATCTTTTCTTCAAATATTCAATGGAAGGAGTGGCCACCATCCTTTATGAGAGTCGTTTG
  GGCTGCCTGGAAAACAGCATCCCACAGCTGACTGTGGAATACATCGAGGCCCTGGAGCTCATGTTTAGCA
  TGTTCAAGACCTCCATGTATGCAGGCGCCATCCCCAGATGGCTTCGCCCCTTCATCCCAAAGCCCTGGCG
  GGAATTCTGCAGGTCCTGGGATGGACTCTTCAAATTCAGCCAAATTCATGTTGACAACAAGTTGAGGGAC
  ATACAGTACCAAATGGACCGAGGCCGGAGGGTGAGCGGGGGACTTCTCACATACCTCTTCCTTAGCCAGG
  CTCTGACGCTGCAGGAGATCTACGCCAACGTGACTGAGATGCTGCTGGCCGGCGTCGACACGACGTCCTT
  CACCTTGTCTTGGACTGTGTACATCCTGGCAAGGCACCCAGAAGTGCAGCAGACGGTGTACCGGGAGATT
  GTGAAGAATTTAGGGGAAAGGCATGTTCCAACTGCAGCTGATGTCCCCAAGGTCCCGCTGGTCAGAGCTC
  TCCTTAAGGAAACCCTGAGGCTGTTTCCAGTGCTGCCAGGGAACGGCCGGGTCACCCAGGAAGACCTGGT
  TATTGGCGGGTATCTGATTCCGAAAGGCACCCAGCTGGCCCTTTGCCACTATGCCACATCGTACCAGGAT
  GAGAACTTCCCTCGGGCCAAGGAGTTCCGGCCTGAGCGCTGGCTGCGGAAAGGAGACTTAGATAGAGTTG
  ACAATTTTGGATCCATCCCCTTTGGTCATGGGGTTCGCAGCTGCATAGGGCGGAGAATTGCAGAACTGGA
  GATTCACCTCGTCGTGATCCAGTTGCTTCAACATTTTGAGATCAAAACATCTTCTCAGACCAATGCTGTT
  CATGCAAAAACCCACGGGCTCCTGACGCCAGGGGGGCCCATCCACGTGCGATTTGTTAACAGAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206930 representing NM_001001665
  Red=Cloning site Green=Tags(s)
  
  MRSVLRQRILKPKDVAIYSGEVNQVIADLIKRIYLLRSQAEDGETVTNVNDLFFKYSMEGVATILYESRL
  GCLENSIPQLTVEYIEALELMFSMFKTSMYAGAIPRWLRPFIPKPWREFCRSWDGLFKFSQIHVDNKLRD
  IQYQMDRGRRVSGGLLTYLFLSQALTLQEIYANVTEMLLAGVDTTSFTLSWTVYILARHPEVQQTVYREI
  VKNLGERHVPTAADVPKVPLVRALLKETLRLFPVLPGNGRVTQEDLVIGGYLIPKGTQLALCHYATSYQD
  ENFPRAKEFRPERWLRKGDLDRVDNFGSIPFGHGVRSCIGRRIAELEIHLVVIQLLQHFEIKTSSQTNAV
  HAKTHGLLTPGGPIHVRFVNRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001665
• ORF Size: 1116 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001665.2, NP_001001665.2
• RefSeq Size: 4725 bp
• RefSeq ORF: 1119 bp
• Locus ID: 339761
• UniProt ID: Q4G0S4
• Cytogenetics: 2q14.3
• Protein Families: Druggable Genome, P450
• MW: 42.5 kDa
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. [provided by RefSeq, Jul 2008]