SHARP1 (BHLHE41) (NM_030762) Human Tagged ORF Clone

CAT#: RC206882

BHLHE41 (Myc-DDK-tagged)-Human basic helix-loop-helix family, member e41 (BHLHE41)

Product Images

Circular map for RC206882

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BHLHE41 (Cat# RC206882, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHLHE41(Cat# TA806394). Positive lysates LY410724 (100ug) and LC410724 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY410724) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206882 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SHARP1
• Synonyms: BHLHB3; DEC2; FNSS1; hDEC2; SHARP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206882 representing NM_030762
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGAAGGAATTCCTCATTTGCAAGAGAGACAGTTACTGGAACATAGAGATTTTATAGGACTGGACT
  ATTCCTCTTTGTATATGTGTAAACCCAAAAGGAGCATGAAACGAGACGACACCAAGGATACCTACAAATT
  ACCGCACAGATTAATAGAAAAGAAAAGAAGAGACCGAATTAATGAATGCATTGCTCAGCTGAAAGATTTA
  CTGCCTGAACATCTGAAATTGACAACTCTGGGACATCTGGAGAAAGCTGTAGTCTTGGAATTAACTTTGA
  AACACTTAAAAGCTTTAACCGCCTTAACCGAGCAACAGCATCAGAAGATAATTGCTTTACAGAATGGGGA
  GCGATCTCTGAAATCGCCCATTCAGTCCGACTTGGATGCGTTCCACTCGGGATTTCAAACATGCGCCAAA
  GAAGTCTTGCAATACCTCTCCCGGTTTGAGAGCTGGACACCCAGGGAGCCGCGGTGTGTCCAGCTGATCA
  ACCACTTGCACGCCGTGGCCACCCAGTTCTTGCCCACCCCGCAGCTGTTGACTCAACAGGTCCCTCTGAG
  CAAAGGCACCGGCGCTCCCTCGGCCGCCGGGTCCGCGGCCGCCCCCTGCCTGGAGCGCGCGGGGCAGAAG
  CTGGAGCCCCTCGCCTACTGCGTGCCCGTCATCCAGCGGACTCAGCCCAGCGCCGAGCTCGCCGCCGAGA
  ACGACACGGACACCGACAGCGGCTACGGCGGCGAAGCCGAGGCCCGGCCGGACCGCGAGAAAGGCAAAGG
  CGCGGGGGCGAGCCGCGTCACCATCAAGCAGGAGCCTCCCGGGGAGGACTCGCCGGCGCCCAAGAGGATG
  AAGCTGGATTCCCGCGGCGGCGGCAGCGGCGGCGGCCCGGGGGGCGGCGCGGTGGCGGCGGCAGCCGCGC
  TTCTGGGGCCCGACCCTGCCGCCGCGGCCGCGCTGCTGAGACCCGACGCCGCCCTGCTCAGCTCGCTGGT
  GGCGTTCGGCGGAGGCGGAGGCGCGCCCTTCCCGCAGCCCGCGGCCGCCGCGGCCCCCTTCTGCCTGCCC
  TTCTGCTTCCTCTCGCCTTCTGCAGCTGCCGCCTACGTGCAGCCCTTCCTGGACAAGAGCGGCCTGGAGA
  AGTATCTGTACCCGGCGGCGGCTGCCGCCCCGTTCCCGCTGCTATACCCCGGCATCCCCGCCCCGGCGGC
  AGCCGCGGCAGCCGCCGCCGCCGCTGCCGCCGCCGCCGCCGCGTTCCCCTGCCTGTCCTCGGTGTTGTCG
  CCCCCTCCCGAGAAGGCGGGCGCCGCCGCCGCGACCCTCCTGCCGCACGAGGTGGCGCCCCTTGGGGCGC
  CGCACCCCCAGCACCCGCACGGCCGCACCCACCTGCCCTTCGCCGGGCCCCGCGAGCCGGGGAACCCGGA
  GAGCTCTGCTCAGGAAGATCCCTCGCAGCCAGGAAAGGAAGCTCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206882 representing NM_030762
  Red=Cloning site Green=Tags(s)
  
  MDEGIPHLQERQLLEHRDFIGLDYSSLYMCKPKRSMKRDDTKDTYKLPHRLIEKKRRDRINECIAQLKDL
  LPEHLKLTTLGHLEKAVVLELTLKHLKALTALTEQQHQKIIALQNGERSLKSPIQSDLDAFHSGFQTCAK
  EVLQYLSRFESWTPREPRCVQLINHLHAVATQFLPTPQLLTQQVPLSKGTGAPSAAGSAAAPCLERAGQK
  LEPLAYCVPVIQRTQPSAELAAENDTDTDSGYGGEAEARPDREKGKGAGASRVTIKQEPPGEDSPAPKRM
  KLDSRGGGSGGGPGGGAVAAAAALLGPDPAAAAALLRPDAALLSSLVAFGGGGGAPFPQPAAAAAPFCLP
  FCFLSPSAAAAYVQPFLDKSGLEKYLYPAAAAAPFPLLYPGIPAPAAAAAAAAAAAAAAAAFPCLSSVLS
  PPPEKAGAAAATLLPHEVAPLGAPHPQHPHGRTHLPFAGPREPGNPESSAQEDPSQPGKEAP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030762
• ORF Size: 1446 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030762.3
• RefSeq Size: 3641 bp
• RefSeq ORF: 1449 bp
• Locus ID: 79365
• UniProt ID: Q9C0J9
• Cytogenetics: 12p12.1
• Domains: HLH, ORANGE
• Protein Families: Transcription Factors
• Protein Pathways: Circadian rhythm - mammal
• MW: 50.3 kDa
• Gene Summary: This gene encodes a basic helix-loop-helix protein expressed in various tissues. The encoded protein can interact with ARNTL or compete for E-box binding sites in the promoter of PER1 and repress CLOCK/ARNTL's transactivation of PER1. This gene is believed to be involved in the control of circadian rhythm and cell differentiation. Defects in this gene are associated with the short sleep phenotype. [provided by RefSeq, Feb 2014]