DENTT (TSPYL2) (NM_022117) Human Tagged ORF Clone

CAT#: RC206828

TSPYL2 (Myc-DDK-tagged)-Human TSPY-like 2 (TSPYL2)

Product Images

Circular map for RC206828

• 

  Western blot validation of overexpression lysate (Cat# LY411789) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206828 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DENTT
• Synonyms: CDA1; CINAP; CTCL; DENTT; HRIHFB2216; NP79; SE204; TSPX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206828 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCGCCCAGATGAGGGGCCTCCGGCCAAGACCCGCCGCCTGAGCAGCTCCGAGTCTCCACAGCGCG
  ACCCGCCCCCGCCGCCGCCGCCGCCGCCGCTCCTCCGACTGCCGCTGCCTCCACCCCAGCAGCGCCCGAG
  GCTCCAGGAGGAAACGGAGGCGGCACAGGTGCTGGCCGATATGAGGGGGGTGGGACTGGGCCCCGCGCTG
  CCCCCGCCGCCTCCCTATGTCATTCTCGAGGAGGGGGGGATCCGCGCATACTTCACGCTCGGTGCTGAGT
  GTCCCGGCTGGGATTCTACCATCGAGTCGGGGTATGGGGAGGCGCCCCCGCCCACGGAGAGCCTGGAAGC
  ACTCCCCACTCCTGAGGCCTCGGGGGGGAGCCTGGAAATCGATTTTCAGGTTGTACAGTCGAGCAGTTTT
  GGTGGAGAGGGGGCCCTAGAAACCTGTAGCGCAGTGGGGTGGGCGCCCCAGAGGTTAGTTGACCCGAAGA
  GCAAGGAAGAGGCGATCATCATAGTGGAGGATGAGGATGAGGATGAGCGGGAGAGTATGAGGAGCAGCAG
  GAGGCGGCGGCGGCGGCGGAGGAGGAAGCAGAGGAAGGTGAAGAGGGAAAGCAGAGAGAGAAATGCCGAG
  AGGATGGAGAGCATCCTGCAGGCACTGGAGGATATTCAGCTGGATCTGGAGGCAGTGAACATCAAGGCAG
  GCAAAGCCTTCCTGCGTCTCAAGCGCAAGTTCATCCAGATGCGAAGACCCTTCCTGGAGCGCAGAGACCT
  CATCATCCAGCATATCCCAGGCTTCTGGGTCAAAGCATTCCTCAACCACCCCAGAATTTCAATTTTGATC
  AACCGACGTGATGAAGACATTTTCCGCTACTTGACCAATCTGCAGGTACAGGATCTCAGACATATCTCCA
  TGGGCTACAAAATGAAGCTGTACTTCCAGACTAACCCCTACTTCACAAACATGGTGATTGTCAAGGAGTT
  CCAGCGCAACCGCTCAGGCCGGCTGGTGTCTCACTCAACCCCAATCCGCTGGCACCGGGGCCAGGAACCC
  CAGGCCCGTCGTCACGGGAACCAGGATGCGAGCCACAGCTTTTTCAGCTGGTTCTCAAACCATAGCCTCC
  CAGAGGCTGACAGGATTGCTGAGATTATCAAGAATGATCTGTGGGTTAACCCTCTACGCTACTACCTGAG
  AGAAAGGGGCTCCAGGATAAAGAGAAAGAAGCAAGAAATGAAGAAACGTAAAACCAGGGGCAGATGTGAG
  GTGGTGATCATGGAAGACGCCCCTGACTATTATGCAGTGGAAGACATTTTCAGCGAGATCTCAGACATTG
  ATGAGACAATTCATGACATCAAGATCTCTGACTTCATGGAGACCACCGACTACTTCGAGACCACTGACAA
  TGAGATAACTGACATCAATGAGAACATCTGCGACAGCGAGAATCCTGACCACAATGAGGTCCCCAACAAC
  GAGACCACTGATAACAACGAGAGTGCTGATGACCACGAAACCACTGACAACAATGAGAGTGCAGATGACA
  ACAACGAGAATCCTGAAGACAATAACAAGAACACTGATGACAACGAAGAGAACCCTAACAACAACGAGAA
  CACTTACGGCAACAACTTCTTCAAAGGTGGCTTCTGGGGCAGCCATGGCAACAACCAGGACAGCAGCGAC
  AGTGACAATGAAGCAGATGAGGCCAGTGATGATGAAGATAATGATGGCAACGAAGGTGACAATGAGGGCA
  GTGATGATGATGGCAATGAAGGTGACAATGAAGGCAGCGATGATGACGACAGAGACATTGAGTACTATGA
  GAAAGTTATTGAAGACTTTGACAAGGATCAGGCTGACTACGAGGACGTGATAGAGATCATCTCAGACGAA
  TCAGTGGAAGAAGAGGGCATTGAGGAAGGCATCCAGCAAGATGAGGACATCTATGAGGAAGGAAACTATG
  AGGAGGAAGGAAGTGAAGATGTCTGGGAAGAAGGGGAAGATTCGGACGACTCTGACCTAGAGGATGTGCT
  TCAGGTCCCAAACGGTTGGGCCAATCCGGGGAAGAGGGGGAAAACCGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206828 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDRPDEGPPAKTRRLSSSESPQRDPPPPPPPPPLLRLPLPPPQQRPRLQEETEAAQVLADMRGVGLGPAL
  PPPPPYVILEEGGIRAYFTLGAECPGWDSTIESGYGEAPPPTESLEALPTPEASGGSLEIDFQVVQSSSF
  GGEGALETCSAVGWAPQRLVDPKSKEEAIIIVEDEDEDERESMRSSRRRRRRRRRKQRKVKRESRERNAE
  RMESILQALEDIQLDLEAVNIKAGKAFLRLKRKFIQMRRPFLERRDLIIQHIPGFWVKAFLNHPRISILI
  NRRDEDIFRYLTNLQVQDLRHISMGYKMKLYFQTNPYFTNMVIVKEFQRNRSGRLVSHSTPIRWHRGQEP
  QARRHGNQDASHSFFSWFSNHSLPEADRIAEIIKNDLWVNPLRYYLRERGSRIKRKKQEMKKRKTRGRCE
  VVIMEDAPDYYAVEDIFSEISDIDETIHDIKISDFMETTDYFETTDNEITDINENICDSENPDHNEVPNN
  ETTDNNESADDHETTDNNESADDNNENPEDNNKNTDDNEENPNNNENTYGNNFFKGGFWGSHGNNQDSSD
  SDNEADEASDDEDNDGNEGDNEGSDDDGNEGDNEGSDDDDRDIEYYEKVIEDFDKDQADYEDVIEIISDE
  SVEEEGIEEGIQQDEDIYEEGNYEEEGSEDVWEEGEDSDDSDLEDVLQVPNGWANPGKRGKTG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022117
• ORF Size: 2079 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022117.4
• RefSeq Size: 2833 bp
• RefSeq ORF: 2082 bp
• Locus ID: 64061
• UniProt ID: Q9H2G4
• Cytogenetics: Xp11.22
• Domains: NAP
• Protein Families: Druggable Genome
• MW: 79.4 kDa
• Gene Summary: This gene encodes a member of the testis-specific protein Y-encoded, TSPY-like/SET/nucleosome assembly protein-1 superfamily. The encoded protein is localized to the nucleolus where it functions in chromatin remodeling and as an inhibitor of cell-cycle progression. This protein may play a role in the suppression of tumor growth. [provided by RefSeq, Sep 2009]