ZNF597 (NM_152457) Human Tagged ORF Clone

CAT#: RC206744

ZNF597 (Myc-DDK-tagged)-Human zinc finger protein 597 (ZNF597)

Product Images

Circular map for RC206744

• 

  Western blot validation of overexpression lysate (Cat# LY407517) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206744 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF597
• Synonyms: HIT-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206744 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCATGCCCCCGACGCCCGAGGCCCAGGGACCAATACTGTTTGAGGATCTGGCTGTGTATTTTT
  CTCAAGAGGAGTGCGTGACTCTGCACCCTGCCCAGAGGTCCCTCAGCAAAGATGGTACAAAAGAGTCTTT
  GGAGGATGCGGCTTTGATGGGAGAGGAAGGCAAGCCTGAGATTAATCAGCAGTTAAGCCTAGAGTCTATG
  GAACTTGACGAGCTTGCCTTAGAAAAGTACCCCATTGCTGCACCCCTTGTCCCTTACCCAGAAAAATCCT
  CTGAGGATGGAGTTGGAAACCCTGAAGCGAAAATATTAAGTGGAACTCCCACTTACAAGAGAAGGGTCAT
  CAGCCTTTTAGTTACCATTGAAAACCACACCCCATTAGTAGAACTCTCTGAATATTTAGGAACCAACACA
  CTTTCTGAAATTCTTGATTCTCCCTGGGAAGGAGCCAAAAATGTGTACAAATGTCCTGAGTGTGACCAAA
  ACTTCAGCGATCATTCATACCTAGTTTTGCATCAGAAAATTCATTCAGGAGAGAAAAAACATAAATGTGG
  TGACTGTGGAAAGATCTTCAATCATAGAGCCAACCTGAGGACACACAGGAGAATCCATACTGGTGAGAAA
  CCTTATAAGTGTGCCAAGTGCAGTGCCAGCTTTCGCCAGCACTCTCATCTATCCCGACACATGAATAGCC
  ACGTAAAGGAGAAGCCCTATACATGTAGCATATGTGGTAGAGGTTTTATGTGGCTCCCAGGATTGGCACA
  GCATCAGAAAAGCCACAGTGCTGAAAACACCTACGAATCTACTAACTGTGATAAACATTTTAATGAGAAA
  CCAAATCTTGCTTTGCCTGAGGAAACATTCGTATCAGGCCCCCAGTACCAGCACACTAAGTGCATGAAGA
  GCTTCAGGCAGTCCTTATATCCTGCCCTTTCCGAGAAGAGCCACGACGAGGACTCTGAACGCTGCAGCGA
  TGATGGGGACAATTTCTTCTCATTCTCAAAATTCAAGCCCTTACAGTGTCCTGACTGTGACATGACCTTT
  CCTTGTTTCTCTGAGCTTATTTCCCATCAGAACATTCATACAGAGGAAAGGCCCCATAAGTGCAAAACAT
  GCGAGGAAAGTTTTGCTTTGGACTCAGAACTTGCATGCCACCAGAAGAGCCACATGCTAGCGGAACCTTT
  TAAATGTACCGTGTGTGGGAAAACTTTCAAGTCGAATTTGCATCTCATTACTCATAAGCGAACTCACATA
  AAAAACACCACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206744 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASMPPTPEAQGPILFEDLAVYFSQEECVTLHPAQRSLSKDGTKESLEDAALMGEEGKPEINQQLSLESM
  ELDELALEKYPIAAPLVPYPEKSSEDGVGNPEAKILSGTPTYKRRVISLLVTIENHTPLVELSEYLGTNT
  LSEILDSPWEGAKNVYKCPECDQNFSDHSYLVLHQKIHSGEKKHKCGDCGKIFNHRANLRTHRRIHTGEK
  PYKCAKCSASFRQHSHLSRHMNSHVKEKPYTCSICGRGFMWLPGLAQHQKSHSAENTYESTNCDKHFNEK
  PNLALPEETFVSGPQYQHTKCMKSFRQSLYPALSEKSHDEDSERCSDDGDNFFSFSKFKPLQCPDCDMTF
  PCFSELISHQNIHTEERPHKCKTCEESFALDSELACHQKSHMLAEPFKCTVCGKTFKSNLHLITHKRTHI
  KNTT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152457
• ORF Size: 1272 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152457.1, NP_689670.1
• RefSeq Size: 1838 bp
• RefSeq ORF: 1275 bp
• Locus ID: 146434
• UniProt ID: Q96LX8
• Cytogenetics: 16p13.3
• Domains: KRAB, zf-C2H2
• Protein Families: Transcription Factors
• MW: 48.1 kDa
• Gene Summary: This gene encodes a protein with multiple zinc finger domains. Loss of the related gene in rodents results in defects in neural development and embryonic lethality in mutant homozygotes. This gene is adjacent to a differentially methylated region (DMR) and is imprinted and maternally expressed. [provided by RefSeq, Nov 2015]