RBMS2 (NM_002898) Human Tagged ORF Clone

CAT#: RC206581

RBMS2 (Myc-DDK-tagged)-Human RNA binding motif, single stranded interacting protein 2 (RBMS2)

Product Images

Circular map for RC206581

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  Western blot validation of overexpression lysate (Cat# LY419043) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206581 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RBMS2 protein (Cat# TP306581). The protein was produced from HEK293T cells transfected with RBMS2 cDNA clone (Cat# RC206581) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RBMS2
• Synonyms: SCR3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206581 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTATCCGTGACTTCCAGGCCCGGGATTTCGACTTTTGGCTACAATAGAAACAACAAGAAGCCAT
  ATGTGTCACTGGCTCAGCAGATGGCACCACCTAGCCCAAGCAACAGTACACCTAACAGCAGTAGTGGAAG
  CAATGGAAATGACCAGCTGAGCAAAACCAACCTATACATCCGAGGATTGCAACCAGGCACTACTGACCAA
  GATCTTGTCAAGCTGTGTCAGCCATATGGCAAGATTGTTTCCACTAAGGCCATACTGGACAAGACCACAA
  ACAAATGTAAAGGCTATGGCTTTGTAGATTTTGACAGCCCTTCAGCAGCACAGAAAGCTGTAACAGCACT
  GAAGGCCAGCGGTGTACAGGCACAGATGGCAAAGCAACAGGAACAGGACCCCACAAATTTATACATCTCA
  AACCTCCCACTGTCAATGGATGAGCAGGAACTGGAGGGGATGCTGAAGCCCTTTGGCCAGGTTATCTCCA
  CCCGTATCCTTCGAGATACCAGTGGGACCAGCAGAGGTGTTGGCTTTGCAAGGATGGAGTCCACAGAGAA
  GTGTGAAGCCATCATCACCCACTTTAATGGAAAATATATTAAGACACCCCCTGGAGTACCAGCCCCATCC
  GATCCCTTGCTTTGCAAATTTGCTGATGGCGGGCCAAAGAAACGACAGAACCAAGGAAAATTTGTGCAAA
  ATGGACGGGCTTGGCCAAGGAATGCAGACATGGGCGTCATGGCCTTGACCTATGACCCCACCACAGCTCT
  TCAGAATGGGTTTTACCCAGCCCCCTATAACATCACCCCCAACAGGATGCTTGCTCAGTCTGCACTCTCC
  CCATACCTTTCCTCTCCTGTGTCTTCGTATCAGAGAGTGACTCAGACATCTCCTCTACAAGTACCTAACC
  CATCCTGGATGCACCACCATTCATACCTCATGCAGCCTTCAGGTTCAGTTCTGACACCAGGGATGGACCA
  TCCCATTTCTCTCCAGCCTGCCTCCATGATGGGACCCCTTACCCAGCAACTGGGCCATCTCTCCCTCAGC
  AGCACAGGCACGTATATGCCGACGGCTGCAGCTATGCAAGGAGCTTACATCTCCCAGTACACCCCTGTGC
  CTTCTTCCAGTGTTTCAGTCGAGGAGAGCAGCGGCCAACAGAACCAAGTGGCAGTGGACGCACCCTCAGA
  GCATGGGGTCTATTCTTTCCAGTTCAACAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206581 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLLSVTSRPGISTFGYNRNNKKPYVSLAQQMAPPSPSNSTPNSSSGSNGNDQLSKTNLYIRGLQPGTTDQ
  DLVKLCQPYGKIVSTKAILDKTTNKCKGYGFVDFDSPSAAQKAVTALKASGVQAQMAKQQEQDPTNLYIS
  NLPLSMDEQELEGMLKPFGQVISTRILRDTSGTSRGVGFARMESTEKCEAIITHFNGKYIKTPPGVPAPS
  DPLLCKFADGGPKKRQNQGKFVQNGRAWPRNADMGVMALTYDPTTALQNGFYPAPYNITPNRMLAQSALS
  PYLSSPVSSYQRVTQTSPLQVPNPSWMHHHSYLMQPSGSVLTPGMDHPISLQPASMMGPLTQQLGHLSLS
  STGTYMPTAAAMQGAYISQYTPVPSSSVSVEESSGQQNQVAVDAPSEHGVYSFQFNK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002898
• ORF Size: 1221 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002898.1
• RefSeq Size: 8504 bp
• RefSeq ORF: 1224 bp
• Locus ID: 5939
• UniProt ID: Q15434
• Cytogenetics: 12q13.3
• Domains: RRM
• MW: 44 kDa
• Gene Summary: The protein encoded by this gene is a member of a small family of proteins which bind single stranded DNA/RNA. These proteins are characterized by the presence of two sets of ribonucleoprotein consensus sequence (RNP-CS) that contain conserved motifs, RNP1 and RNP2, originally described in RNA binding proteins, and required for DNA binding. The RBMS proteins have been implicated in such diverse functions as DNA replication, gene transcription, cell cycle progression and apoptosis. This protein was isolated by phenotypic complementation of cdc2 and cdc13 mutants of yeast and is thought to suppress cdc2 and cdc13 mutants through the induction of translation of cdc2. [provided by RefSeq, Jul 2008]