GNA14 (NM_004297) Human Tagged ORF Clone

CAT#: RC206547

GNA14 (Myc-DDK-tagged)-Human guanine nucleotide binding protein (G protein), alpha 14 (GNA14)

Product Images

Circular map for RC206547

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GNA14 (Cat# RC206547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GNA14 (Cat# TA812023). Positive lysates LY418087 (100ug) and LC418087 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY418087) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206547 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GNA14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206547 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGCTGCTGCTGCCTGTCCGCGGAGGAGAAGGAGTCGCAGCGCATCAGCGCGGAGATCGAGCGAC
  AGCTTCGTCGGGACAAGAAGGACGCGCGCCGTGAGCTTAAGCTGCTGCTGCTGGGAACTGGTGAAAGTGG
  GAAAAGCACCTTTATCAAGCAGATGAGAATTATCCATGGGTCTGGTTACAGCGACGAAGACAGAAAGGGG
  TTCACGAAGCTGGTTTACCAAAACATATTCACCGCCATGCAAGCCATGATCAGAGCGATGGACACGCTAA
  GGATACAGTATGTGTGTGAACAGAATAAGGAAAATGCCCAGATAATCAGAGAAGTGGAAGTGGACAAGGT
  CTCCATGCTCTCCAGGGAGCAGGTGGAGGCCATCAAGCAGCTCTGGCAAGATCCAGGCATCCAGGAGTGT
  TACGACAGGAGGAGGGAGTACCAGCTGTCGGACTCTGCCAAATATTACCTGACTGACATTGACCGCATCG
  CCACACCATCATTCGTGCCTACCCAACAAGATGTGCTTCGCGTCCGAGTGCCCACCACCGGCATCATTGA
  GTATCCATTTGACTTGGAAAACATCATCTTTCGGATGGTGGATGTTGGTGGCCAACGATCGGAAAGACGG
  AAGTGGATTCACTGCTTTGAGAGTGTCACCTCCATTATTTTCTTGGTTGCTCTGAGTGAATATGACCAGG
  TCCTGGCTGAGTGTGACAACGAGAATCGCATGGAAGAGAGCAAAGCCTTATTTAAAACCATCATCACCTA
  CCCCTGGTTTCTGAATTCGTCTGTGATTTTATTCTTGAACAAGAAGGATCTTTTGGAAGAGAAAATCATG
  TACTCTCATCTAATTAGCTATTTCCCAGAATACACAGGACCGAAACAGGATGTCAGAGCTGCCAGAGACT
  TTATCCTGAAGCTTTACCAAGATCAGAATCCTGACAAAGAGAAAGTCATCTACTCTCACTTCACATGTGC
  TACAGATACAGACAATATTCGCTTTGTGTTTGCTGCTGTCAAAGACACAATTCTACAGCTAAACCTAAGG
  GAATTCAACCTTGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206547 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGCCCLSAEEKESQRISAEIERQLRRDKKDARRELKLLLLGTGESGKSTFIKQMRIIHGSGYSDEDRKG
  FTKLVYQNIFTAMQAMIRAMDTLRIQYVCEQNKENAQIIREVEVDKVSMLSREQVEAIKQLWQDPGIQEC
  YDRRREYQLSDSAKYYLTDIDRIATPSFVPTQQDVLRVRVPTTGIIEYPFDLENIIFRMVDVGGQRSERR
  KWIHCFESVTSIIFLVALSEYDQVLAECDNENRMEESKALFKTIITYPWFLNSSVILFLNKKDLLEEKIM
  YSHLISYFPEYTGPKQDVRAARDFILKLYQDQNPDKEKVIYSHFTCATDTDNIRFVFAAVKDTILQLNLR
  EFNLV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004297
• ORF Size: 1065 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004297.4
• RefSeq Size: 2492 bp
• RefSeq ORF: 1068 bp
• Locus ID: 9630
• UniProt ID: O95837
• Cytogenetics: 9q21.2
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway
• MW: 41.6 kDa
• Gene Summary: This gene encodes a member of the guanine nucleotide-binding, or G protein family. G proteins are heterotrimers consisting of alpha, beta and gamma subunits. The encoded protein is a member of the alpha family of G proteins, more specifically the alpha q subfamily of G proteins. The encoded protein may play a role in pertussis-toxin resistant activation of phospholipase C-beta and its downstream effectors.[provided by RefSeq, Feb 2009]