FMO3 (NM_006894) Human Tagged ORF Clone

CAT#: RC206506

FMO3 (Myc-DDK-tagged)-Human flavin containing monooxygenase 3 (FMO3), transcript variant 1

Product Images

Circular map for RC206506

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY FMO3 (Cat# RC206506, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FMO3 (Cat# TA808310)(1:2000). Positive lysates LY416335 (100ug) and LC416335 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY424145) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212376 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified FMO3 protein (Cat# TP306506). The protein was produced from HEK293T cells transfected with FMO3 cDNA clone (Cat# RC206506) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FMO3
• Synonyms: dJ127D3.1; FMOII; TMAU
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206506 representing NM_006894
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAAGAAAGTGGCCATCATTGGAGCTGGTGTGAGTGGCTTGGCCTCCATCAGGAGCTGTCTGGAAG
  AGGGGCTGGAGCCCACCTGCTTTGAGAAGAGCAATGACATTGGGGGCCTGTGGAAATTTTCAGACCATGC
  AGAGGAGGGCAGGGCTAGCATTTACAAATCAGTCTTTTCCAACTCTTCCAAAGAGATGATGTGTTTCCCA
  GACTTCCCATTTCCCGATGACTTCCCCAACTTTATGCACAACAGCAAGATCCAGGAATATATCATTGCAT
  TTGCCAAAGAAAAGAACCTCCTGAAGTACATACAATTTAAGACATTTGTATCCAGTGTAAATAAACATCC
  TGATTTTGCAACTACTGGCCAGTGGGATGTTACCACTGAAAGGGATGGTAAAAAAGAATCGGCTGTCTTT
  GATGCTGTAATGGTTTGTTCCGGACATCATGTGTATCCCAACCTACCAAAAGAGTCCTTTCCAGGACTAA
  ACCACTTTAAAGGCAAATGCTTCCACAGCAGGGACTATAAAGAACCAGGTGTATTCAATGGAAAGCGTGT
  CCTGGTGGTTGGCCTGGGGAATTCGGGCTGTGATATTGCCACAGAACTCAGCCGCACAGCAGAACAGGTC
  ATGATCAGTTCCAGAAGTGGCTCCTGGGTGATGAGCCGGGTCTGGGACAATGGTTATCCTTGGGACATGC
  TGCTCGTCACTCGATTTGGAACCTTCCTCAAGAACAATTTACCGACAGCCATCTCTGACTGGTTGTACAT
  GAAGCAGATGAATGCAAGATTCAAGCATGAAAACTATGGCTTGATGCCTTTAAATGGAGTCCTGAGGAAA
  GAGCCTGTATTTAACGATGAGCTCCCAGCAAGCATTCTGTGTGGCATTGTGTCCGTAAAGCCTAACGTGA
  AGGAATTCACAGAGACCTCGGCCATTTTTGAGGATGGGACCATATTTGAGGGCATTGACTGTGTAATCTT
  TGCAACAGGGTATAGTTTTGCCTACCCCTTCCTTGATGAGTCTATCATCAAAAGCAGAAACAATGAGATC
  ATTTTATTTAAAGGAGTATTTCCTCCTCTACTTGAGAAGTCAACCATAGCAGTGATTGGCTTTGTCCAGT
  CCCTTGGGGCTGCCATTCCCACAGTTGACCTCCAGTCCCGCTGGGCAGCACAAGTAATAAAGGGAACTTG
  TACTTTGCCTTCTATGGAAGACATGATGAATGATATTAATGAGAAAATGGAGAAAAAGCGCAAATGGTTT
  GGCAAAAGCGAGACCATACAGACAGATTACATTGTTTATATGGATGAACTCTCCTCCTTCATTGGGGCAA
  AGCCCAACATCCCATGGCTGTTTCTCACAGATCCCAAATTGGCCATGGAAGTTTATTTTGGCCCTTGTAG
  TCCCTACCAGTTTAGGCTGGTGGGCCCAGGGCAGTGGCCAGGAGCCAGAAATGCCATACTGACCCAGTGG
  GACCGGTCGTTGAAACCCATGCAGACACGAGTGGTCGGGAGACTTCAGAAGCCTTGCTTCTTTTTCCATT
  GGCTGAAGCTCTTTGCAATTCCTATTCTGTTAATCGCTGTTTTCCTTGTGTTGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206506 representing NM_006894
  Red=Cloning site Green=Tags(s)
  
  MGKKVAIIGAGVSGLASIRSCLEEGLEPTCFEKSNDIGGLWKFSDHAEEGRASIYKSVFSNSSKEMMCFP
  DFPFPDDFPNFMHNSKIQEYIIAFAKEKNLLKYIQFKTFVSSVNKHPDFATTGQWDVTTERDGKKESAVF
  DAVMVCSGHHVYPNLPKESFPGLNHFKGKCFHSRDYKEPGVFNGKRVLVVGLGNSGCDIATELSRTAEQV
  MISSRSGSWVMSRVWDNGYPWDMLLVTRFGTFLKNNLPTAISDWLYMKQMNARFKHENYGLMPLNGVLRK
  EPVFNDELPASILCGIVSVKPNVKEFTETSAIFEDGTIFEGIDCVIFATGYSFAYPFLDESIIKSRNNEI
  ILFKGVFPPLLEKSTIAVIGFVQSLGAAIPTVDLQSRWAAQVIKGTCTLPSMEDMMNDINEKMEKKRKWF
  GKSETIQTDYIVYMDELSSFIGAKPNIPWLFLTDPKLAMEVYFGPCSPYQFRLVGPGQWPGARNAILTQW
  DRSLKPMQTRVVGRLQKPCFFFHWLKLFAIPILLIAVFLVLT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006894
• ORF Size: 1596 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006894.3
• RefSeq Size: 2053 bp
• RefSeq ORF: 1599 bp
• Locus ID: 2328
• UniProt ID: P31513
• Cytogenetics: 1q24.3
• Domains: FMO-like
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Drug metabolism - cytochrome P450
• MW: 59.9 kDa
• Gene Summary: Flavin-containing monooxygenases (FMO) are an important class of drug-metabolizing enzymes that catalyze the NADPH-dependent oxygenation of various nitrogen-,sulfur-, and phosphorous-containing xenobiotics such as therapeutic drugs, dietary compounds, pesticides, and other foreign compounds. The human FMO gene family is composed of 5 genes and multiple pseudogenes. FMO members have distinct developmental- and tissue-specific expression patterns. The expression of this FMO3 gene, the major FMO expressed in adult liver, can vary up to 20-fold between individuals. This inter-individual variation in FMO3 expression levels is likely to have significant effects on the rate at which xenobiotics are metabolised and, therefore, is of considerable interest to the pharmaceutical industry. This transmembrane protein localizes to the endoplasmic reticulum of many tissues. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. Mutations in this gene cause the disorder trimethylaminuria (TMAu) which is characterized by the accumulation and excretion of unmetabolized trimethylamine and a distinctive body odor. In healthy individuals, trimethylamine is primarily converted to the non odorous trimethylamine N-oxide.[provided by RefSeq, Jan 2016]