KCNE1L (KCNE5) (NM_012282) Human Tagged ORF Clone

CAT#: RC206502

KCNE1L (Myc-DDK-tagged)-Human KCNE1-like (KCNE1L)

Product Images

Circular map for RC206502

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  Western blot validation of overexpression lysate (Cat# LY415850) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206502 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified KCNE5 protein (Cat# TP306502). The protein was produced from HEK293T cells transfected with KCNE5 cDNA clone (Cat# RC206502) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCNE1L
• Synonyms: KCNE1L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206502 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTGCAGCGAGAGCCAGCGGCTGCGAACCCTTCTGAGCCGCCTGTTGCTCGAGCTGCACCACCGGG
  GTAATGCCAGCGGCTTGGGCGCTGGCCCTCGTCCCAGCATGGGCATGGGGGTCGTGCCTGACCCTTTCGT
  GGGCCGCGAGGTGACCAGCGCCAAGGGCGACGACGCCTATCTCTACATCCTGCTCATCATGATCTTCTAC
  GCCTGCTTGGCCGGAGGCCTCATCCTGGCCTACACCCGCTCCCGTAAGCTCGTCGAGGCCAAGGACGAGC
  CGTCCCAGGCTTGCGCCGAGCACGAATGGGCCCCGGGAGGCGCCCTGACCGCCGACGCCGAGGCTGCCGC
  GGGCTCCCAGGCCGAGGGCCGCCGCCAGCTTGCCTCCGAGGGGCTGCCTGCCCTCGCCCAGGGCGCTGAG
  CGGGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206502 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNCSESQRLRTLLSRLLLELHHRGNASGLGAGPRPSMGMGVVPDPFVGREVTSAKGDDAYLYILLIMIFY
  ACLAGGLILAYTRSRKLVEAKDEPSQACAEHEWAPGGALTADAEAAAGSQAEGRRQLASEGLPALAQGAE
  RV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012282
• ORF Size: 426 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012282.4
• RefSeq Size: 1465 bp
• RefSeq ORF: 429 bp
• Locus ID: 23630
• UniProt ID: Q9UJ90
• Cytogenetics: Xq23
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• MW: 15 kDa
• Gene Summary: This gene encodes a member of a family of single pass transmembrane domain proteins that function as ancillary subunits to voltage-gated potassium channels. Members of this family affect diverse processes in potassium channel regulation, including ion selectivity, voltage dependence, and anterograde recycling from the plasma membrane. Variants of this gene are associated with idiopathic ventricular fibrillation and Brugada syndrome. [provided by RefSeq, Nov 2016]