PHACTR4 (NM_001048183) Human Tagged ORF Clone

CAT#: RC206425

PHACTR4 (Myc-DDK-tagged)-Human phosphatase and actin regulator 4 (PHACTR4), transcript variant 1

Product Images

Circular map for RC206425

• 

  Western blot validation of overexpression lysate (Cat# LY420773) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206425 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PHACTR4
• Synonyms: PPP1R124
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206425 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGATCCATTTGAGGAAGCAGACCAGCCCACTACAGAGCCAGGCATGGTCCTGGACAGTGTGGAAG
  CAGGAGACACAACACCTCCTACCAAAAGGAAGAGCAAGTTCTCAGGCTTTGGCAAGATCTTCAAGCCCTG
  GAAATGGAGGAAAAAAAAAAGTAGTGATAAATTTAAAGAGACTTCAGAAGTTTTAGAACGGAAAATATCT
  ATGCGAAAGCCAAGAGAAGAGCTGGTTAAAAGAGGGGTTCTGTTGGAAGACCCTGAGCAAGGTGGTGAGG
  ATCCAGGAAAGCCAAGCGATGCCATGTTAAAGAATGGCCATACCACCCCCATAGGGAATGCCAGATCATC
  TAGTCCAGTCCAAGTAGAGGAAGAGCCAGTAAGATTAGCAAGTCTTAGGAAAGCTATTCCAGAAGAGGAC
  CTAAAGAAACGACTAGGCTCAACTGGAAGCCAGCCTAATTCTGAAGCAGAGTCTGTTCCTGAGAATGTAC
  CCAAACCACCTTTACTTCCTCCCAAAAGACCCTTGTCCTCTTCTCATGAAGCAAGTGAAGGGCAAGCAAA
  GGATGCCACTTCCTCTGGCGGCACGGCAAGGTTCATCATCTCCACCTCCATCACCACAGCACCCGCTGCC
  ACCACTGCTGCCACAAGCCTTGCAAAGACTGTTAATCTCTCTGTCACCCCTTCCCCAGCACCCAGGACTC
  TGCCTGCTGCTCCTGCCAGCACTAACACTACTGCTACCCCAAGCCTCACTCATATGGTCCCTGCCAAGCA
  GCCCCCTATCCCTCCCCCTAAACCAGCTCACAGAAATAGCAACCCTGTCATTGCTGAACTGTCCCAAGCA
  ATAAACAGTGGTACATTGTTATCAAAACCGTCCCCACCCTTACCACCTAAGAGAGGCATTCCATCAACCT
  CAGTACCCACCTTGGAGTCTGCTGCTGCCATCACCACAAAAACACCAAGTGATGAAAGAGAGAAGAGCAC
  GTGTTCTATGGGCTCGGAACTACTACCAATGATCTCACCTCGCTCTCCGTCCCCCCCACTGCCTACTCAT
  ATACCTCCAGAGCCTCCACGCACCCCTCCATTCCCTGCTAAGACTTTTCAAGTTGTGCCAGAAATTGAGT
  TTCCACCATCCTTAGATCTACACCAGGAGATTCCCCAGCAGGAAGATCAGAAAAAGGAAGTCCCCAAGAG
  GATACTGGACCAGAACTTTGGGGAGCCCCATATACCCTCTAGGCTGCCTCCACTCCCACTGCATATTCGA
  ATCCAGCAGGCCCTCACCAGCCCACTTCCCATGACTCCTATTCTGGAGGGTTCTCACAGAGCTCATTCGT
  TGCTTTTTGAAAACAGTGACAGCTTTTCTGAGGACAGCAGTACGCTGGGTCGGACCAGGTCTCTTCCCAT
  CACTATTGAAATGCTAAAAGTTCCAGACGATGAAGAAGAAGAGGAGCAAACCTGTCCATCCACATTCAGT
  GAAGAAATGACACCTACCTCAGTCATTCCTAAATTACCACAGTGTCTACGGGAGGAAGAAGAGAAGGAGA
  GCGACTCTGATTCAGAAGGTCCCATTCAGTACCGAGATGAAGAAGATGAAGATGAAAGCTATCAGAGTGC
  TCTCGCCAACAAAGTGAAGAGGAAAGACACACTGGCAATGAAGTTGAACCACAGACCCAGTGAACCAGAG
  TTGAACCTGAATTCTTGGCCTTGTAAAAGCAAGGAGGAGTGGAATGAAATACGGCACCAGATTGGAAACA
  CACTGATCCGGCGACTGAGTCAAAGACCAACACCAGAAGAACTAGAACAACGCAATATATTGCAACCTAA
  AAATGAAGCTGATCGTCAGGCAGAAAAACGAGAAATTAAACGTCGGCTCACTAGAAAGCTCAGTCAAAGG
  CCAACTGTCGCTGAACTCCTTGCCAGGAAGATTCTGAGGTTTAATGAATATGTAGAGGTAACAGATGCTC
  AAGATTATGACCGGCGAGCCGACAAACCTTGGACCAAACTGACCCCTGCTGACAAGGCTGCCATAAGAAA
  AGAATTAAATGAATTTAAAAGCTCCGAGATGGAGGTTCATGAAGAGAGCAAACATTTTACACGCTACCAT
  CGTCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206425 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEDPFEEADQPTTEPGMVLDSVEAGDTTPPTKRKSKFSGFGKIFKPWKWRKKKSSDKFKETSEVLERKIS
  MRKPREELVKRGVLLEDPEQGGEDPGKPSDAMLKNGHTTPIGNARSSSPVQVEEEPVRLASLRKAIPEED
  LKKRLGSTGSQPNSEAESVPENVPKPPLLPPKRPLSSSHEASEGQAKDATSSGGTARFIISTSITTAPAA
  TTAATSLAKTVNLSVTPSPAPRTLPAAPASTNTTATPSLTHMVPAKQPPIPPPKPAHRNSNPVIAELSQA
  INSGTLLSKPSPPLPPKRGIPSTSVPTLESAAAITTKTPSDEREKSTCSMGSELLPMISPRSPSPPLPTH
  IPPEPPRTPPFPAKTFQVVPEIEFPPSLDLHQEIPQQEDQKKEVPKRILDQNFGEPHIPSRLPPLPLHIR
  IQQALTSPLPMTPILEGSHRAHSLLFENSDSFSEDSSTLGRTRSLPITIEMLKVPDDEEEEEQTCPSTFS
  EEMTPTSVIPKLPQCLREEEEKESDSDSEGPIQYRDEEDEDESYQSALANKVKRKDTLAMKLNHRPSEPE
  LNLNSWPCKSKEEWNEIRHQIGNTLIRRLSQRPTPEELEQRNILQPKNEADRQAEKREIKRRLTRKLSQR
  PTVAELLARKILRFNEYVEVTDAQDYDRRADKPWTKLTPADKAAIRKELNEFKSSEMEVHEESKHFTRYH
  RP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001048183
• ORF Size: 2106 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001048183.3
• RefSeq Size: 6225 bp
• RefSeq ORF: 2109 bp
• Locus ID: 65979
• UniProt ID: Q8IZ21
• Cytogenetics: 1p35.3
• MW: 78.2 kDa
• Gene Summary: This gene encodes a member of the phosphatase and actin regulator (PHACTR) family. Other PHACTR family members have been shown to inhibit protein phosphatase 1 (PP1) activity, and the homolog of this gene in the mouse has been shown to interact with actin and PP1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]