SLC19A3 (NM_025243) Human Tagged ORF Clone

CAT#: RC206409

SLC19A3 (Myc-DDK-tagged)-Human solute carrier family 19, member 3 (SLC19A3)

Product Images

Circular map for RC206409

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  Western blot validation of overexpression lysate (Cat# LY410805) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206409 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SLC19A3 protein (Cat# TP306409). The protein was produced from HEK293T cells transfected with SLC19A3 cDNA clone (Cat# RC206409) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC19A3
• Synonyms: BBGD; THMD2; thTr-2; THTR2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206409 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTGTTACAGAACTTCACTAAGCAGTTCCTGGATTTACCCCACTGTGATCCTCTGCTTATTTGGTT
  TTTTCTCCATGATGAGACCCTCAGAACCGTTCCTTATCCCATATTTATCTGGACCAGATAAAAACCTGAC
  CAGTGCAGAGATAACAAATGAGATCTTCCCCGTTTGGACATACTCCTACCTGGTGCTGCTGCTGCCTGTG
  TTTGTCCTCACCGATTATGTCCGCTACAAGCCAGTCATCATCTTGCAAGGTATCAGTTTCATCATTACCT
  GGCTGCTGCTGTTGTTTGGCCAAGGAGTGAAGACCATGCAGGTTGTAGAGTTCTTCTATGGGATGGTCAC
  CGCCGCCGAGGTGGCCTACTACGCCTACATATACAGCGTGGTCAGCCCCGAGCACTACCAGAGAGTGAGC
  GGCTACTGCAGGAGCGTCACGCTGGCCGCCTACACAGCAGGGTCGGTGCTGGCTCAACTCTTGGTATCCC
  TGGCGAACATGTCGTACTTTTACCTCAACGTCATATCCTTGGCCTCTGTCTCCGTGGCTTTCCTTTTCTC
  ACTTTTCCTACCAATGCCCAAGAAAAGCATGTTTTTTCATGCAAAACCCAGCAGAGAAATAAAGAAGTCA
  TCAAGCGTGAATCCAGTATTAGAGGAAACTCACGAAGGTGAAGCACCAGGCTGTGAAGAGCAGAAACCCA
  CATCAGAAATACTCAGCACTTCAGGGAAGCTGAATAAGGGCCAGCTGAACAGCCTGAAACCAAGCAATGT
  GACTGTGGACGTTTTTGTGCAGTGGTTCCAAGATTTGAAGGAGTGCTACTCCTCAAAACGTCTTTTCTAC
  TGGTCTCTATGGTGGGCTTTCGCCACAGCAGGTTTTAACCAGGTTTTGAACTATGTTCAAATCCTGTGGG
  ATTACAAGGCGCCATCCCAAGATTCTTCCATCTATAATGGGGCCGTAGAAGCTATTGCAACCTTTGGAGG
  GGCTGTGGCTGCCTTTGCAGTGGGTTATGTGAAAGTCAACTGGGACCTTCTGGGAGAGCTGGCTCTGGTG
  GTCTTCTCAGTTGTCAATGCCGGTTCTTTATTTCTCATGCATTACACAGCCAATATCTGGGCGTGCTATG
  CTGGCTATTTGATATTCAAGTCCAGCTATATGCTTCTTATAACCATAGCAGTATTTCAGATTGCAGTTAA
  TCTGAATGTGGAACGCTATGCCTTGGTATTTGGAATCAACACCTTTATTGCCTTGGTGATTCAGACCATC
  ATGACTGTGATTGTAGTAGATCAGAGAGGGCTCAACTTGCCAGTCAGCATTCAGTTTTTAGTTTATGGGA
  GCTATTTTGCAGTAATTGCTGGAATTTTCCTAATGAGAAGCATGTATATTACCTACTCAACCAAATCCCA
  GAAGGATGTACAGAGCCCTGCTCCAAGTGAGAATCCAGATGTGTCTCACCCAGAGGAAGAGAGTAATATC
  ATCATGTCAACAAAACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206409 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDCYRTSLSSSWIYPTVILCLFGFFSMMRPSEPFLIPYLSGPDKNLTSAEITNEIFPVWTYSYLVLLLPV
  FVLTDYVRYKPVIILQGISFIITWLLLLFGQGVKTMQVVEFFYGMVTAAEVAYYAYIYSVVSPEHYQRVS
  GYCRSVTLAAYTAGSVLAQLLVSLANMSYFYLNVISLASVSVAFLFSLFLPMPKKSMFFHAKPSREIKKS
  SSVNPVLEETHEGEAPGCEEQKPTSEILSTSGKLNKGQLNSLKPSNVTVDVFVQWFQDLKECYSSKRLFY
  WSLWWAFATAGFNQVLNYVQILWDYKAPSQDSSIYNGAVEAIATFGGAVAAFAVGYVKVNWDLLGELALV
  VFSVVNAGSLFLMHYTANIWACYAGYLIFKSSYMLLITIAVFQIAVNLNVERYALVFGINTFIALVIQTI
  MTVIVVDQRGLNLPVSIQFLVYGSYFAVIAGIFLMRSMYITYSTKSQKDVQSPAPSENPDVSHPEEESNI
  IMSTKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025243
• ORF Size: 1488 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025243.4
• RefSeq Size: 3775 bp
• RefSeq ORF: 1491 bp
• Locus ID: 80704
• UniProt ID: Q9BZV2
• Cytogenetics: 2q36.3
• Domains: Folate_carrier
• Protein Families: Transmembrane
• MW: 55.7 kDa
• Gene Summary: This gene encodes a ubiquitously expressed transmembrane thiamine transporter that lacks folate transport activity. Mutations in this gene cause biotin-responsive basal ganglia disease (BBGD); a recessive disorder manifested in childhood that progresses to chronic encephalopathy, dystonia, quadriparesis, and death if untreated. Patients with BBGD have bilateral necrosis in the head of the caudate nucleus and in the putamen. Administration of high doses of biotin in the early progression of the disorder eliminates pathological symptoms while delayed treatment results in residual paraparesis, mild cognitive disability, or dystonia. Administration of thiamine is ineffective in the treatment of this disorder. Experiments have failed to show that this protein can transport biotin. Mutations in this gene also cause a Wernicke's-like encephalopathy.[provided by RefSeq, Jan 2010]