SUPV3L1 (NM_003171) Human Tagged ORF Clone

CAT#: RC206350

SUPV3L1 (Myc-DDK-tagged)-Human suppressor of var1, 3-like 1 (S. cerevisiae) (SUPV3L1)

Product Images

Circular map for RC206350

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  Western blot validation of overexpression lysate (Cat# LY418860) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206350 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SUPV3L1 protein (Cat# TP306350). The protein was produced from HEK293T cells transfected with SUPV3L1 cDNA clone (Cat# RC206350) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SUPV3L1
• Synonyms: SUV3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206350 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTTCTCCCGTGCCCTATTGTGGGCTCGGCTCCCGGCGGGGCGCCAGGCTGGCCACCGGGCAGCCA
  TCTGCTCTGCCCTTCGTCCCCACTTTGGGCCCTTTCCCGGGGTTCTGGGGCAAGTTTCTGTCCTTGCCAC
  CGCCTCCTCCTCTGCCTCCGGTGGCTCCAAAATACCAAACACGTCCTTGTTCGTGCCCCTGACTGTGAAA
  CCTCAGGGCCCCAGCGCCGACGGCGACGTCGGGGCCGAGCTAACCCGGCCTCTGGACAAGAATGAAGTAA
  AGAAGGTCTTAGACAAATTTTACAAGAGGAAAGAAATTCAGAAACTGGGTGCTGATTATGGACTTGATGC
  TCGTCTCTTCCACCAAGCTTTCATAAGCTTTAGAAATTATATTATGCAGTCTCATTCCCTGGATGTGGAC
  ATTCACATTGTTTTGAATGATATTTGCTTCGGTGCAGCTCATGCGGATGATTTATTCCCATTTTTCTTGA
  GACATGCCAAACAAATATTTCCTGTGTTGGACTGTAAGGATGATCTACGTAAAATCAGTGACTTAAGAAT
  ACCACCTAACTGGTACCCAGATGCTAGAGCCATGCAGCGGAAGATAATATTTCATTCAGGCCCCACAAAC
  AGTGGAAAGACTTATCACGCAATCCAGAAATACTTCTCAGCAAAGTCTGGAGTGTATTGTGGCCCTCTAA
  AATTACTGGCACATGAGATCTTCGAAAAGAGTAATGCTGCTGGTGTGCCATGTGACTTGGTGACAGGTGA
  AGAGCGTGTGACAGTTCAGCCAAATGGGAAACAGGCTTCACATGTTTCTTGTACAGTTGAGATGTGCAGT
  GTTACAACTCCTTATGAAGTGGCTGTAATTGATGAAATTCAAATGATTAGAGATCCAGCCAGAGGATGGG
  CCTGGACCAGAGCACTTCTAGGACTGTGTGCTGAAGAGGTTCATTTGTGTGGAGAACCTGCTGCTATTGA
  CCTGGTGATGGAGCTTATGTACACAACGGGGGAGGAAGTGGAGGTTCGAGACTATAAGAGGCTTACCCCC
  ATTTCTGTGCTGGACCATGCACTAGAATCTTTAGATAACCTTCGGCCTGGGGACTGCATTGTCTGTTTTA
  GCAAGAATGATATTTATTCTGTGAGTCGGCAGATTGAAATTCGGGGATTAGAATCAGCTGTTATATATGG
  CAGTCTCCCACCTGGGACCAAACTTGCTCAAGCAAAAAAGTTTAATGATCCCAATGACCCATGCAAAATC
  TTGGTTGCTACAGATGCAATTGGCATGGGACTTAATTTGAGCATAAGGAGAATTATTTTTTACTCCCTTA
  TAAAGCCCAGTATCAATGAAAAGGGAGAGAGAGAACTAGAACCAATCACAACCTCTCAAGCCCTGCAGAT
  TGCTGGCAGAGCTGGCAGATTCAGCTCACGGTTTAAAGAAGGAGAGGTTACAACAATGAATCATGAAGAT
  CTCAGTTTATTAAAGGAAATTTTGAAGAGGCCTGTGGATCCTATAAGGGCAGCTGGTCTTCATCCAACTG
  CTGAGCAGATTGAAATGTTTGCCTACCATCTCCCTGATGCAACACTGTCCAATCTCATTGATATTTTTGT
  AGACTTTTCACAAGTTGATGGGCAGTATTTTGTCTGCAATATGGATGATTTTAAATTTTCTGCAGAGTTG
  ATCCAGCATATTCCACTAAGTCTGCGAGTGAGGTATGTTTTCTGCACAGCTCCTATCAACAAGAAGCAGC
  CTTTTGTGTGTTCTTCACTGTTACAGTTTGCCAGGCAGTATAGCAGGAATGAGCCCCTGACCTTTGCATG
  GTTACGCCGATACATCAAATGGCCTTTACTTCCACCTAAGAATATTAAAGACCTCATGGATCTTGAAGCT
  GTCCACGATGTCTTGGATCTTTACTTGTGGCTAAGCTACCGATTTATGGATATGTTTCCAGATGCCAGCC
  TTATTCGAGATCTCCAGAAAGAACTAGATGGTATTATCCAAGATGGTGTGCACAATATCACTAAATTGAT
  TAAAATGTCTGAGACGCATAAGCTGTTGAATTTGGAGGGCTTTCCATCAGGGAGCCAGTCACGATTGTCA
  GGAACCTTAAAGAGCCAAGCTAGAAGGACACGCGGCACCAAAGCTCTAGGGAGTAAAGCTACTGAGCCAC
  CCAGCCCCGATGCAGGAGAGCTGTCCCTTGCTTCCAGATTGGTGCAGCAAGGACTCCTCACTCCAGACAT
  GCTGAAACAGCTAGAAAAAGAGTGGATGACACAACAAACTGAACACAACAAAGAAAAAACAGAGTCTGGG
  ACTCATCCAAAAGGGACGAGAAGAAAGAAGAAGGAACCTGATTCGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206350 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSFSRALLWARLPAGRQAGHRAAICSALRPHFGPFPGVLGQVSVLATASSSASGGSKIPNTSLFVPLTVK
  PQGPSADGDVGAELTRPLDKNEVKKVLDKFYKRKEIQKLGADYGLDARLFHQAFISFRNYIMQSHSLDVD
  IHIVLNDICFGAAHADDLFPFFLRHAKQIFPVLDCKDDLRKISDLRIPPNWYPDARAMQRKIIFHSGPTN
  SGKTYHAIQKYFSAKSGVYCGPLKLLAHEIFEKSNAAGVPCDLVTGEERVTVQPNGKQASHVSCTVEMCS
  VTTPYEVAVIDEIQMIRDPARGWAWTRALLGLCAEEVHLCGEPAAIDLVMELMYTTGEEVEVRDYKRLTP
  ISVLDHALESLDNLRPGDCIVCFSKNDIYSVSRQIEIRGLESAVIYGSLPPGTKLAQAKKFNDPNDPCKI
  LVATDAIGMGLNLSIRRIIFYSLIKPSINEKGERELEPITTSQALQIAGRAGRFSSRFKEGEVTTMNHED
  LSLLKEILKRPVDPIRAAGLHPTAEQIEMFAYHLPDATLSNLIDIFVDFSQVDGQYFVCNMDDFKFSAEL
  IQHIPLSLRVRYVFCTAPINKKQPFVCSSLLQFARQYSRNEPLTFAWLRRYIKWPLLPPKNIKDLMDLEA
  VHDVLDLYLWLSYRFMDMFPDASLIRDLQKELDGIIQDGVHNITKLIKMSETHKLLNLEGFPSGSQSRLS
  GTLKSQARRTRGTKALGSKATEPPSPDAGELSLASRLVQQGLLTPDMLKQLEKEWMTQQTEHNKEKTESG
  THPKGTRRKKKEPDSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003171
• ORF Size: 2358 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003171.5
• RefSeq Size: 2530 bp
• RefSeq ORF: 2361 bp
• Locus ID: 6832
• UniProt ID: Q8IYB8
• Cytogenetics: 10q22.1
• Domains: helicase_C
• MW: 88 kDa
• Gene Summary: Major helicase player in mitochondrial RNA metabolism. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. ATPase and ATP-dependent multisubstrate helicase, able to unwind double-stranded (ds) DNA and RNA, and RNA/DNA heteroduplexes in the 5'-to-3' direction. Plays a role in the RNA surveillance system in mitochondria; regulates the stability of mature mRNAs, the removal of aberrantly formed mRNAs and the rapid degradation of non coding processing intermediates. Also implicated in recombination and chromatin maintenance pathways. May protect cells from apoptosis. Associates with mitochondrial DNA.[UniProtKB/Swiss-Prot Function]