GRPEL2 (NM_152407) Human Tagged ORF Clone

CAT#: RC206315

GRPEL2 (Myc-DDK-tagged)-Human GrpE-like 2, mitochondrial (E. coli) (GRPEL2), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC206315

• 

  Western blot validation of overexpression lysate (Cat# LY407575) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206315 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GRPEL2
• Synonyms: Mt-GrpE#2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206315 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGTACGGTCGCTGTGGGCGGGCCGGCTGCGGGTGCAGCGCCTACTGGCCTGGAGTGCAGCGTGGG
  AGAGCAAGGGATGGCCGCTTCCATTCAGCACTGCCACCCAGAGAACTGCTGGTGAGGACTGCCGTTCTGA
  GGACCCTCCTGATGAGCTTGGGCCCCCTCTTGCTGAACGAGCCTTAAGGGTAAAAGCTGTTAAACTGGAG
  AAAGAAGTCCAAGATTTAACAGTGAGATACCAGAGAGCTATAGCTGATTGTGAAAACATAAGGAGGCGAA
  CCCAGAGATGTGTGGAAGACGCCAAGATATTTGGAATCCAGAGTTTCTGTAAGGACTTGGTGGAGGTGGC
  TGACATTTTGGAGAAGACTACAGAGTGCATTTCTGAAGAATCGGAGCCTGAGGACCAAAAGCTCACTCTG
  GAGAAGGTCTTCCGAGGGTTGTTGCTTTTAGAAGCAAAGCTGAAAAGTGTGTTTGCCAAGCATGGCCTGG
  AGAAACTGACACCCATTGGTGACAAATATGACCCCCATGAGCATGAACTCATCTGTCATGTGCCAGCTGG
  TGTTGGGGTGCAGCCTGGCACCGTGGCATTAGTAAGACAAGATGGCTACAAACTTCATGGCCGCACCATT
  AGGCTTGCCCGAGTGGAAGTGGCAGTGGAGTCTCAGAGAAGACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206315 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAVRSLWAGRLRVQRLLAWSAAWESKGWPLPFSTATQRTAGEDCRSEDPPDELGPPLAERALRVKAVKLE
  KEVQDLTVRYQRAIADCENIRRRTQRCVEDAKIFGIQSFCKDLVEVADILEKTTECISEESEPEDQKLTL
  EKVFRGLLLLEAKLKSVFAKHGLEKLTPIGDKYDPHEHELICHVPAGVGVQPGTVALVRQDGYKLHGRTI
  RLARVEVAVESQRRL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152407
• ORF Size: 675 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152407.4
• RefSeq Size: 4105 bp
• RefSeq ORF: 678 bp
• Locus ID: 134266
• UniProt ID: Q8TAA5
• Cytogenetics: 5q32
• MW: 25.4 kDa
• Gene Summary: Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins. Stimulates ATPase activity of mt-HSP70. May also serve to modulate the interconversion of oligomeric (inactive) and monomeric (active) forms of mt-HSP70 (By similarity).[UniProtKB/Swiss-Prot Function]