DGCR2 (NM_005137) Human Tagged ORF Clone

CAT#: RC206306

DGCR2 (Myc-DDK-tagged)-Human DiGeorge syndrome critical region gene 2 (DGCR2), transcript variant 1

Product Images

Circular map for RC206306

• 

  Western blot validation of overexpression lysate (Cat# LY417498) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206306 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DGCR2
• Synonyms: DGS-C; IDD; LAN; SEZ-12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206306 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCCCAAGGCAGACAGCGGCGCCTTCCTGCTGCTCTTCCTGCTCGTGCTCACTGTCACCGAGCCGC
  TGCGGCCAGAGCTGCGGTGCAACCCTGGGCAGTTTGCGTGTCGCAGCGGCACCATCCAGTGCATCCCCCT
  CCCCTGGCAGTGTGACGGCTGGGCGACTTGCGAGGATGAGAGCGACGAAGCCAACTGTCCAGAAGTGACC
  GGGGAGGTGCGTCCTCATCATGGGAAGGAGGCTGTGGATCCGCGGCAGGGGCGGGCCAGAGGAGGCGACC
  CTTCGCACTTCCACGCGGTGAACGTGGCGCAGCCCGTTCGCTTCAGCAGTTTCCTAGGGAAGTGCCCGAC
  AGGGTGGCACCACTACGAAGGCACGGCCAGCTGCTACCGGGTCTACCTGAGCGGGGAGAACTACTGGGAT
  GCCGCGCAGACCTGCCAGCGCCTGAATGGCTCTCTCGCCACCTTCTCCACTGACCAGGAGCTGCGCTTTG
  TCCTGGCCCAGGAATGGGACCAGCCCGAGCGGAGCTTTGGTTGGAAGGACCAGCGCAAGTTGTGGGTTGG
  CTATCAGTATGTTATCACTGGCCGGAACCGCTCCTTGGAAGGTCGCTGGGAGGTGGCATTCAAAGGCTCT
  TCAGAGGTGTTCCTGCCCCCAGACCCCATCTTTGCCTCGGCCATGTCTGAGAACGACAACGTGTTCTGTG
  CCCAGCTTCAGTGCTTCCATTTCCCCACCCTGCGGCACCACGACCTCCACAGCTGGCACGCCGAGAGCTG
  CTACGAGAAGTCTTCATTTCTGTGTAAAAGAAGTCAAACATGTGTTGACATCAAGGACAACGTGGTGGAT
  GAAGGGTTCTACTTCACCCCTAAGGGGGACGACCCATGCCTGAGCTGCACCTGCCATGGAGGGGAGCCTG
  AGATGTGTGTGGCTGCTCTCTGTGAGAGGCCCCAGGGCTGCCAACAGTACCGCAAGGACCCCAAAGAGTG
  CTGCAAGTTCATGTGTCTGGACCCAGATGGCAACAGTCTGTTTGACTCCATGGCCAGCGGGATGCGCCTG
  GTCGTCAGCTGCATCTCCTCCTTCCTCATCCTGTCACTGCTGCTCTTCATGGTCCACCGGCTGCGCCAGC
  GGCGCCGGGAGCGCATCGAGTCCCTGATTGGAGCAAACTTGCACCACTTCAACCTCGGCCGCAGGATCCC
  TGGCTTTGATTACGGCCCAGACGGGTTTGGCACGGGCCTCACGCCGCTGCATCTTTCTGACGACGGAGAG
  GGTGGGACTTTCCATTTCCACGACCCTCCACCTCCCTACACGGCATACAAGTACCCGGACATCGGCCAGC
  CCGACGACCCTCCGCCGCCCTACGAGGCCTCCATCCACCCGGACAGTGTGTTCTATGACCCTGCAGACGA
  TGATGCTTTTGAGCCTGTGGAGGTCAGCCTGCCAGCCCCTGGGGATGGTGGGAGTGAAGGTGCATTACTC
  CGGCGCCTGGAGCAGCCTCTGCCCACTGCGGGGGCCTCTCTGGCAGACCTGGAAGACTCTGCCGACAGCA
  GCAGCGCCATGCTCGTGCCCCCTGACCCTGCCCAGAGCGGGAGCACCCCAGCTGCAGAGGCACTGCCAGG
  GGGTGGCCGCCACAGCCGCAGCTCCCTCAATACTGTGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206306 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVPKADSGAFLLLFLLVLTVTEPLRPELRCNPGQFACRSGTIQCIPLPWQCDGWATCEDESDEANCPEVT
  GEVRPHHGKEAVDPRQGRARGGDPSHFHAVNVAQPVRFSSFLGKCPTGWHHYEGTASCYRVYLSGENYWD
  AAQTCQRLNGSLATFSTDQELRFVLAQEWDQPERSFGWKDQRKLWVGYQYVITGRNRSLEGRWEVAFKGS
  SEVFLPPDPIFASAMSENDNVFCAQLQCFHFPTLRHHDLHSWHAESCYEKSSFLCKRSQTCVDIKDNVVD
  EGFYFTPKGDDPCLSCTCHGGEPEMCVAALCERPQGCQQYRKDPKECCKFMCLDPDGNSLFDSMASGMRL
  VVSCISSFLILSLLLFMVHRLRQRRRERIESLIGANLHHFNLGRRIPGFDYGPDGFGTGLTPLHLSDDGE
  GGTFHFHDPPPPYTAYKYPDIGQPDDPPPPYEASIHPDSVFYDPADDDAFEPVEVSLPAPGDGGSEGALL
  RRLEQPLPTAGASLADLEDSADSSSAMLVPPDPAQSGSTPAAEALPGGGRHSRSSLNTVV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005137
• ORF Size: 1650 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005137.3
• RefSeq Size: 4504 bp
• RefSeq ORF: 1653 bp
• Locus ID: 9993
• UniProt ID: P98153
• Cytogenetics: 22q11.21
• Domains: VWC, CLECT, ldl_recept_a
• Protein Families: Druggable Genome, Transmembrane
• MW: 60.8 kDa
• Gene Summary: Deletions of the 22q11.2 have been associated with a wide range of developmental defects (notably DiGeorge syndrome, velocardiofacial syndrome, conotruncal anomaly face syndrome and isolated conotruncal cardiac defects) classified under the acronym CATCH 22. The DGCR2 gene encodes a novel putative adhesion receptor protein, which could play a role in neural crest cells migration, a process which has been proposed to be altered in DiGeorge syndrome. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]