RNF190 (MARCH10) (NM_152598) Human Tagged ORF Clone

CAT#: RC206212

MARCH10 (Myc-DDK-tagged)-Human membrane-associated ring finger (C3HC4) 10 (MARCH10), transcript variant 1

Product Images

Circular map for RC206212

• 

  Western blot validation of overexpression lysate (Cat# LY420299) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218712 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RNF190
• Synonyms: MARCH-X; MARCH10; RNF190
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206212 representing NM_152598.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTTGCATGACGCAAGGGACAGGCAGAAGTTCTTCAGCGATGTTCAGTATCTGCGGGACATGCAGCAT
  AAGGTGGACTCTGAGTATCAGGCTTGTCTGAGACGACAGGAATATAGAAGAGACCCAAATGAGAAGAAA
  CGCGATCAGTTTTGGGGGCAAGAGACAAGTTTTGAGAGATCCCGGTTTTCTAGCAGGTCATCTTCCAAA
  CAGAGTTCTAGTGAGGAGGATGCTCTAACTGAACCAAGGTCATCTATCAAGATATCTGCATTTAAGTGT
  GACTCCAAACTTCCAGCAATTGACCAAACATCAGTCAAGCAGAAACATAAAAGTACCATGACTGTAAGG
  AAAGCAGAGAAAGTGGACCCCAGCGAACCCTCTCCAGCAGACCAAGCACCAATGGTTTTATTAAGAAAG
  AGGAAGCCAAACCTGAGGAGATTTACAGTCAGCCCAGAATCGCACAGCCCGAGAGCATCTGGGGACAGA
  AGCAGACAGAAACAGCAGTGGCCTGCAAAGGTGCCGGTTCCCAGGGGAGCAGATCAAGTAGTTCAACAA
  GAAGGCCTGATGTGTAACACTAAGCTGAAGAGGCCAAATCAAGAGAGAAGAAACTTGGTCCCATCGTCA
  CAGCCAATGACTGAGAACGCCCCTGATAGAGCCAAAAAAGGAGACCCGAGTGCTCCTTCCCAGAGTGAG
  CTGCATCCAGCCCTGTCCCAGGCCTTCCAAGGAAAAAATAGTCCTCAAGTATTGAGTGAGTTCTCGGGG
  CCACCACTCACACCCACCACTGTAGGAGGGCCAAGAAAGGCATCATTTAGGTTCCGAGATGAAGACTTT
  TATTCCATTTTGTCATTGAACAGCAGAAGAGAAAGCGATGACACTGAAGAGGAAACCCAGTCTGAAGAA
  TGTCTGTGGGTTGGTGTGCGCTCACCCTGTTCTCCTTCCCATCACAAAAGAAGTAGATTTGGGGGGACA
  TCGACCCCTCAGGCCAAAAATAAAAATTTTGAAGAAAATGCTGAAAATTGCAGAGGTCATTCTTCAAGA
  AGAAGTGAGCCCAGTCATGGCTCATTGAGAATAAGCAATGCAATGGAGCCAGCAACAGAGCGGCCTTCT
  GCTGGGCAAAGGTTGTCCCAAGACCCCGGGCTGCCTGATAGGGAATCTGCTACAGAGAAGGACAGAGGT
  GGCAGTGAAAATGCGAAAAAGAGCCCTCTTTCGTGGGACACCAAATCCGAGCCCAGACAAGAGGTTGGT
  GTCAATGCTGAAAATGTATGGAGTGATTGTATTTCTGTGGAACATAGGCCTGGCACCCATGATTCTGAA
  GGATACTGGAAAGACTATTTAAACAGTTCTCAAAATTCTCTTGACTACTTTATTTCTGGCAGACCAATA
  TCTCCAAGATCATCAGTGAATTCATCCTATAACCCTCCTGCATCATTCATGCATTCTGCTCTGAGAGAT
  GATATTCCAGTAGACTTGTCAATGTCATCGACTTCAGTTCACAGCTCAGACTCAGAGGGAAACTCAGGG
  TTTCATGTTTGCCAACCACTGTCTCCCATTAGAAATAGGACTCCATTTGCCAGTGCCGAAAACCATAAT
  TATTTCCCAGTAAACAGTGCACACGAATTTGCTGTCAGGGAAGCAGAAGATACTACTTTAACAAGCCAG
  CCTCAGGGGGCTCCACTATATACAGATCTCTTACTAAATCCACAGGGCAATTTGTCCTTAGTGGATTCT
  TCCAGCTCCTCTCCATCAAGAATGAACTCAGAAGGCCATTTGCATGTGTCTGGGTCTCTGCAAGAAAAT
  ACACCATTTACTTTCTTTGCAGTGTCTCATTTCCCAAATCAAAATGATAATGGGAGCAGGATGGCAGCC
  TCTGGTTTCACAGATGAAAAGGAAACCAGTAAGATAAAGGCAGACCCTGAGAAACTCAAGAAATTGCAA
  GAAAGTCTCCTGGAGGAGGACTCCGAGGAGGAGGGAGACTTGTGTCGCATCTGTCAGATAGCCGGGGGT
  TCCCCAAGCAACCCCCTCCTGGAGCCTTGCGGCTGTGTGGGAAGCCTGCAGTTTGTTCATCAAGAGTGC
  CTGAAAAAGTGGCTGAAAGTGAAAATAACATCAGGAGCAGATCTTGGTGCCGTGAAGACCTGTGAGATG
  TGTAAGCAAGGCCTGCTGGTTGACCTGGGTGACTTTAACATGATTGAGTTCTACCAGAAGCACCAGCAA
  TCTCAGGCACAAAACGAGCTGATGAATTCAGGCTTGTACCTGGTGCTGCTGCTTCACCTCTATGAGCAG
  AGGTTTGCAGAACTCATGAGGCTCAACCACAACCAGGTGGAAAGAGAGAGGTTGTCAAGAAATTACCCA
  CAACCCAGAACAGAGGAAAATGAAAATTCGGAGTTGGGAGATGGAAATGAAGGCAGCATTTCTCAAAGC
  CAGGTCGTC
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >Peptide sequence encoded by RC206212
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MLHDARDRQKFFSDVQYLRDMQHKVDSEYQACLRRQEYRRDPNEKKRDQFWGQETSFERSRFSSRSSSK
  QSSSEEDALTEPRSSIKISAFKCDSKLPAIDQTSVKQKHKSTMTVRKAEKVDPSEPSPADQAPMVLLRK
  RKPNLRRFTVSPESHSPRASGDRSRQKQQWPAKVPVPRGADQVVQQEGLMCNTKLKRPNQERRNLVPSS
  QPMTENAPDRAKKGDPSAPSQSELHPALSQAFQGKNSPQVLSEFSGPPLTPTTVGGPRKASFRFRDEDF
  YSILSLNSRRESDDTEEETQSEECLWVGVRSPCSPSHHKRSRFGGTSTPQAKNKNFEENAENCRGHSSR
  RSEPSHGSLRISNAMEPATERPSAGQRLSQDPGLPDRESATEKDRGGSENAKKSPLSWDTKSEPRQEVG
  VNAENVWSDCISVEHRPGTHDSEGYWKDYLNSSQNSLDYFISGRPISPRSSVNSSYNPPASFMHSALRD
  DIPVDLSMSSTSVHSSDSEGNSGFHVCQPLSPIRNRTPFASAENHNYFPVNSAHEFAVREAEDTTLTSQ
  PQGAPLYTDLLLNPQGNLSLVDSSSSSPSRMNSEGHLHVSGSLQENTPFTFFAVSHFPNQNDNGSRMAA
  SGFTDEKETSKIKADPEKLKKLQESLLEEDSEEEGDLCRICQIAGGSPSNPLLEPCGCVGSLQFVHQEC
  LKKWLKVKITSGADLGAVKTCEMCKQGLLVDLGDFNMIEFYQKHQQSQAQNELMNSGLYLVLLLHLYEQ
  RFAELMRLNHNQVERERLSRNYPQPRTEENENSELGDGNEGSISQSQVV
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152598
• ORF Size: 2424 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 3153 bp
• RefSeq ORF: 2427 bp
• Locus ID: 162333
• UniProt ID: Q8NA82
• Cytogenetics: 17q23.2
• MW: 90.5 kDa
• Gene Summary: MARCH10 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Morokuma et al., 2007 [PubMed 17604280]).[supplied by OMIM, Apr 2010]