C5orf25 (SIMC1) (NM_198567) Human Tagged ORF Clone

CAT#: RC206208

SIMC1 (Myc-DDK-tagged)-Human chromosome 5 open reading frame 25 (C5orf25)

Product Images

Circular map for RC206208

• 

  Western blot validation of overexpression lysate (Cat# LY404861) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206208 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C5orf25
• Synonyms: C5orf25; OOMA1; PLEIAD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206208 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACCAGCATCTGCTTCTGGTGAGGACCTCAGGAAGCTTCCAACCATGGCAGAGGTGAATGGGGAGC
  AGGACTTCATTGACTTAACTAGAGAGACCAGACCAAGGACAAAAGATCGCAGTGGACTGTATGTGATTGA
  CCTGACAAGAGCTGAGGGAGAAAATAGACCTATTGCCACTCTTGACTTAACTTTAGAACCTGTCACTCCT
  TCCCAGAAGGAGCCAACCAGTCTTCAGACATGTGCCAGCCTCTCTGGCAAAGCGGTGATGGAAGGGCACG
  TGGACAGAAGCTCTCAGCCTACAGCACGGAGAATCATTAACAGTGATCCTGTAGATTTGGACCTAGTGGA
  AGAAAACACCTTTGTAGGTCCCCCACCCGCTACATCCATCAGTGGAGGCTCTGTTTATCCAACAGAGCCT
  AATTGTAGCTCAGCCACATTCACAGGTAACCTCAGCTTCTTGGCAAGTCTACAGCTGTCTTCAGATGTTA
  GCTCCCTCTCCCCAACAAGCAATAATAGTAGGAGCAGCAGCAGCAGCAGCAATCAAAAAGCACCCTTGCC
  ATGCCCACAGCAAGATGTATCTCGCCCACCACAGGCCTTGCCGTGCCCCCTGCGACCTTTGCCATGCCCA
  CCGAGAGCCTCACCATGTCCACCACGAGCCTCCTCATGCCCACCACGAGCCTTGTCATGCCCATCACAAA
  CCATGCAGTGCCAACTACCAGCTCTAACTCACCCACCTCAAGAAGTGCCATGCCCTCGGCAGAATATCCC
  AGGCCCACCTCAAGACTCTCTGGGCCTACCTCAAGATGTGCCAGGGCTGCCTCAAAGCATATTACATCCA
  CAAGATGTGGCATACCTGCAAGACATGCCACGGTCACCAGGAGATGTGCCACAGTCACCAAGTGATGTTT
  CACCGTCACCAGATGCACCACAGTCACCAGGGGGCATGCCACACTTACCGGGAGATGTGTTACATTCACC
  TGGAGACATGCCACACTCATCAGGGGACGTGACACACTCACCTAGAGACATCCCTCACTTACCAGGAGAC
  AGGCCTGACTTTACCCAGAATGATGTACAGAACCGTGACATGCCTATGGATATCTCAGCTCTGTCCTCTC
  CAAGCTGCTCTCCCAGACCACAGTCTGAAACTCCCTTAGAGAAAGTTCCTTGGCTCTCTGTCATGGAAAC
  CCCAGCCAGAAAAGAAATATCACTGTCAGAGCCTGCCAAACCTGGGTCTGCCCACGTACAATCACGAACA
  CCACAAGGTGGGTTGTACAACAGACCATGCCTGCATAGACTGAAGTACTTCTTACGTCCTCCGGTTCATC
  ACCTCTTCTTTCAGACGCTAATACCGGATAAAGACACAAGAGAGAACAAGGGTCAAAGATTAGAACCCAT
  CCCTCATCGAAGACTAAGAATGGTAACAAATACCATTGAAGAGAATTTTCCTCTGGGGACTGTGCAGTTT
  TTGATGGACTTTGTGTCACCCCAGCATTACCCACCAAGAGAAATCGTGGCTCACATCATCCAGAAAATCT
  TGCTCAGTGGCTCTGAGACTGTGGATGTCCTAAAGGAGGCCTACATGCTTCTCATGAAAATTCAACAGCT
  ACATCCAGCCAATGCCAAGACAGTGGAGTGGGACTGGAAACTGCTCACCTATGTCATGGAGGAAGAGGGA
  CAAACTCTGCCTGGGCGAGTCCTTTTCCTGCGTTATGTCGTTCAGACCCTAGAAGATGACTTTCAGCAGA
  CCCTGAGGAGGCAACGGCAGCACCTGCAGCAATCCATTGCAAACATGGTGCTTTCCTGTGACAAGCAGCC
  CCACAATGTCAGGGATGTTATCAAGTGGCTGGTCAAAGCAGTAACTGAAGATGGATTGACTCAGCCCCCA
  AATGGAAATCAAACGTCTTCAGGAACAGGAATCTTGAAAGCCAGCAGTAGCCACCCTTCTTCCCAGCCCA
  ACCTGACAAAGAACACCAATCAGCTGATTGTGTGCCAGCTTCAGAGGATGCTCTCCATAGCCGTAGAGGT
  GGACAGGACCCCCACCTGCAGCTCCAATAAAATTGCCGAGATGATGTTTGGGTTTGTGCTGGACATTCCT
  GAGAGGAGCCAGAGAGAAATGTTCTTTACTACCATGGAAAGCCACCTTCTGCGCTGCAAAGTGTTAGAAA
  TCATATTCCTCCACAGCTGTGAGACACCCACCCGCCTGCCTCTGTCTCTGGCCCAGGCCCTCTACTTTCT
  GAATAATTCTACGTCACTGCTCAAGTGTCAGTCAGATAAAAGCCAGTGGCAGACTTGGGACGAATTGGTT
  GAGCGTCTGCAGTTTCTGCTGTCCAGTTATCAACATGTTTTAAGAGAACACTTAAGGAGTTCCGTGATCG
  ACCGAAAGGACTTAATAATCAAAAGGATTAAGCCCAAACCCCAGCAAGGAGATGACATCACAGTGGTAGA
  CGTAGAGAAGCAGATTGAGGCCTTCCGCAGCCGCCTGATCCAGATGCTGGGGGAGCCTCTTGTCCCCCAA
  CTCCAAGACAAAGTGCACTTGTTGAAGCTCCTGCTCTTCTATGCTGCGGACTTGAACCCTGATGCAGAGC
  CCTTTCAAAAGGGCTGGAGCGGCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206208 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPASASGEDLRKLPTMAEVNGEQDFIDLTRETRPRTKDRSGLYVIDLTRAEGENRPIATLDLTLEPVTP
  SQKEPTSLQTCASLSGKAVMEGHVDRSSQPTARRIINSDPVDLDLVEENTFVGPPPATSISGGSVYPTEP
  NCSSATFTGNLSFLASLQLSSDVSSLSPTSNNSRSSSSSSNQKAPLPCPQQDVSRPPQALPCPLRPLPCP
  PRASPCPPRASSCPPRALSCPSQTMQCQLPALTHPPQEVPCPRQNIPGPPQDSLGLPQDVPGLPQSILHP
  QDVAYLQDMPRSPGDVPQSPSDVSPSPDAPQSPGGMPHLPGDVLHSPGDMPHSSGDVTHSPRDIPHLPGD
  RPDFTQNDVQNRDMPMDISALSSPSCSPRPQSETPLEKVPWLSVMETPARKEISLSEPAKPGSAHVQSRT
  PQGGLYNRPCLHRLKYFLRPPVHHLFFQTLIPDKDTRENKGQRLEPIPHRRLRMVTNTIEENFPLGTVQF
  LMDFVSPQHYPPREIVAHIIQKILLSGSETVDVLKEAYMLLMKIQQLHPANAKTVEWDWKLLTYVMEEEG
  QTLPGRVLFLRYVVQTLEDDFQQTLRRQRQHLQQSIANMVLSCDKQPHNVRDVIKWLVKAVTEDGLTQPP
  NGNQTSSGTGILKASSSHPSSQPNLTKNTNQLIVCQLQRMLSIAVEVDRTPTCSSNKIAEMMFGFVLDIP
  ERSQREMFFTTMESHLLRCKVLEIIFLHSCETPTRLPLSLAQALYFLNNSTSLLKCQSDKSQWQTWDELV
  ERLQFLLSSYQHVLREHLRSSVIDRKDLIIKRIKPKPQQGDDITVVDVEKQIEAFRSRLIQMLGEPLVPQ
  LQDKVHLLKLLLFYAADLNPDAEPFQKGWSGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198567
• ORF Size: 2616 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198567.2
• RefSeq Size: 2087 bp
• RefSeq ORF: 1374 bp
• Locus ID: 375484
• UniProt ID: Q8NDZ2
• Cytogenetics: 5q35.2
• MW: 97 kDa
• Gene Summary: Inhibits the protease activity of CAPN3.[UniProtKB/Swiss-Prot Function]