AADACL1 (NCEH1) (NM_020792) Human Tagged ORF Clone

CAT#: RC206188

NCEH1 (Myc-DDK-tagged)-Human neutral cholesterol ester hydrolase 1 (NCEH1), transcript variant 2

Product Images

Circular map for RC206188

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  Western blot validation of overexpression lysate (Cat# LY412304) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206188 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NCEH1 protein (Cat# TP306188). The protein was produced from HEK293T cells transfected with NCEH1 cDNA clone (Cat# RC206188) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AADACL1
• Synonyms: AADACL1; NCEH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206188 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAGCTGCCGCGGGCAGAAAGTTGCCGGAGGTCTCCGGGTGGTATCGCCCTTTCCTCTTTGCCAGC
  CCGCTGGCGAGCCGAGCCGGGGCAAGATGAGGTCGTCCTGTGTCCTGCTCACCGCCCTGGTGGCGCTGGC
  CGCCTATTACGTCTACATCCCGCTGCCTGGCTCCGTGTCCGACCCCTGGAAGCTGATGCTGCTGGACGCC
  ACTTTCCGGGGTGCACAGCAAGTGAGTAACCTGATCCACTACCTGGGACTGAGCCATCACCTGCTGGCAC
  TGAATTTTATCATTGTTTCTTTTGGCAAAAAAAGCGCGTGGTCTTCTGCCCAAGTGAAGGTGACCGACAC
  AGACTTTGATGGTGTGGAAGTCAGAGTGTTTGAAGGCCCTCCGAAGCCCGAAGAGCCACTGAAACGCAGC
  GTCGTTTATATCCACGGAGGAGGCTGGGCCTTGGCAAGTGCAAAAATCAGGTATTATGATGAGCTGTGTA
  CAGCAATGGCTGAGGAATTGAATGCTGTCATTGTTTCCATTGAATACAGGCTAGTTCCAAAGGTTTATTT
  TCCTGAGCAAATTCATGATGTTGTACGGGCCACAAAGTATTTCCTGAAGCCAGAAGTCTTACAGAAGTAT
  ATGGTTGATCCAGGCAGAATTTGCATTTCTGGTGACAGTGCTGGTGGAAATCTGGCTGCTGCCCTTGGAC
  AACAGTTTACTCAAGATGCCAGCCTAAAAAATAAGCTCAAACTACAAGCTTTAATTTATCCAGTTCTTCA
  AGCTTTAGATTTTAACACACCATCTTATCAGCAAAATGTGAACACCCCAATCCTGCCCCGCTATGTCATG
  GTGAAGTATTGGGTGGACTACTTCAAAGGCAACTATGACTTTGTGCAGGCAATGATCGTTAACAATCACA
  CTTCACTTGATGTGGAAGAGGCTGCTGCTGTCAGGGCCCGTCTAAACTGGACATCCCTCTTGCCTGCATC
  CTTCACAAAGAACTACAAGCCTGTTGTACAGACCACAGGCAATGCCAGGATTGTCCAGGAGCTTCCTCAG
  TTGCTGGATGCCCGCTCCGCCCCACTCATTGCAGACCAGGCAGTGCTGCAGCTCCTCCCAAAGACCTACA
  TTCTGACGTGTGAGCATGATGTCCTCAGAGACGATGGCATCATGTATGCCAAGCGTTTGGAGAGTGCCGG
  TGTGGAGGTGACCCTGGATCACTTTGAGGATGGCTTTCACGGATGTATGATTTTCACTAGCTGGCCCACC
  AACTTCTCAGTGGGAATCCGGACTAGGAATAGTTACATCAAGTGGCTAGATCAAAACCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206188 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSCRGQKVAGGLRVVSPFPLCQPAGEPSRGKMRSSCVLLTALVALAAYYVYIPLPGSVSDPWKLMLLDA
  TFRGAQQVSNLIHYLGLSHHLLALNFIIVSFGKKSAWSSAQVKVTDTDFDGVEVRVFEGPPKPEEPLKRS
  VVYIHGGGWALASAKIRYYDELCTAMAEELNAVIVSIEYRLVPKVYFPEQIHDVVRATKYFLKPEVLQKY
  MVDPGRICISGDSAGGNLAAALGQQFTQDASLKNKLKLQALIYPVLQALDFNTPSYQQNVNTPILPRYVM
  VKYWVDYFKGNYDFVQAMIVNNHTSLDVEEAAAVRARLNWTSLLPASFTKNYKPVVQTTGNARIVQELPQ
  LLDARSAPLIADQAVLQLLPKTYILTCEHDVLRDDGIMYAKRLESAGVEVTLDHFEDGFHGCMIFTSWPT
  NFSVGIRTRNSYIKWLDQNL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020792
• ORF Size: 1320 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020792.4
• RefSeq Size: 4294 bp
• RefSeq ORF: 1227 bp
• Locus ID: 57552
• UniProt ID: Q6PIU2
• Cytogenetics: 3q26.31
• Protein Families: Transmembrane
• MW: 49.1 kDa
• Gene Summary: Hydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. May be responsible for cholesterol ester hydrolysis in macrophages, thereby contributing to the development of atherosclerosis. Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds. May contribute to cancer pathogenesis by promoting tumor cell migration.[UniProtKB/Swiss-Prot Function]