CSNK1A1L (NM_145203) Human Tagged ORF Clone

CAT#: RC206077

CSNK1A1L (Myc-DDK-tagged)-Human casein kinase 1, alpha 1-like (CSNK1A1L)

Product Images

Circular map for RC206077

• 

  Western blot validation of overexpression lysate (Cat# LY407942) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206077 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CSNK1A1L
• Synonyms: CK1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206077 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAAACAACAGCGGCTCCAAAGCCGAACTCGTTGTGGGAGGGAAATACAAACTGGTGCGGAAGATCG
  GGTCTGGCTCCTTTGGAGACGTTTATCTGGGCATCACCACCACCAACGGCGAGGAAGTAGCAGTGAAGCT
  GGAATCTCAGAAGGTCAAGCACCCCCAGTTGCTGTATGAGAGCAAACTCTACACGATTCTTCAAGGTGGG
  GTTGGCATCCCCCACATGCACTGGTATGGTCAGGAAAAAGACAACAATGTGCTAGTCATGGACCTTCTGG
  GACCCAGCCTCGAAGACCTCTTTAATTTCTGTTCAAGAAGGTTCACCATGAAAACTGTACTTATGTTAGC
  CGACCAGATGATCAGCAGAATTGAATACGTGCATACAAAGAATTTTCTACACCGAGACATTAAACCAGAT
  AACTTCCTGATGGGTACTGGGCGTCACTGTAATAAGTTGTTCCTTATTGATTTTGGTTTGGCCAAAAAGT
  ACAGAGACAACAGGACCAGGCAACACATACCGTACAGAGAAGATAAACACCTCATTGGCACTGTCCGATA
  TGCCAGCATCAATGCACATCTTGGTATTGAGCAGAGCCGCCGAGATGACATGGAATCCTTAGGCTACGTT
  TTCATGTATTTTAATAGAACCAGCCTGCCGTGGCAAGGACTAAAGGCTATGACAAAAAAACAAAAATATG
  AAAAGATTAGTGAGAAGAAGATGTCCACCCCTGTTGAAGTTTTATGTAAGGGGTTTCCTGCAGAATTCGC
  CATGTACTTGAACTACTGTCGTGGGCTGCGCTTTGAGGAAGTCCCAGATTACATGTATCTGAGGCAGCTA
  TTCCGCATTCTTTTCAGGACCCTGAACCACCAATATGACTACACATTTGATTGGACGATGTTAAAGCAGA
  AAGCAGCACAGCAGGCAGCCTCTTCCAGTGGGCAGGGTCAGCAGGCCCAAACCCAGACAGGCAAGCAAAC
  TGAAAAAAACAAGAATAATGTGAAAGATAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206077 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTNNSGSKAELVVGGKYKLVRKIGSGSFGDVYLGITTTNGEEVAVKLESQKVKHPQLLYESKLYTILQGG
  VGIPHMHWYGQEKDNNVLVMDLLGPSLEDLFNFCSRRFTMKTVLMLADQMISRIEYVHTKNFLHRDIKPD
  NFLMGTGRHCNKLFLIDFGLAKKYRDNRTRQHIPYREDKHLIGTVRYASINAHLGIEQSRRDDMESLGYV
  FMYFNRTSLPWQGLKAMTKKQKYEKISEKKMSTPVEVLCKGFPAEFAMYLNYCRGLRFEEVPDYMYLRQL
  FRILFRTLNHQYDYTFDWTMLKQKAAQQAASSSGQGQQAQTQTGKQTEKNKNNVKDN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145203
• ORF Size: 1011 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145203.4
• RefSeq Size: 2405 bp
• RefSeq ORF: 1014 bp
• Locus ID: 122011
• UniProt ID: Q8N752
• Cytogenetics: 13q13.3
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Hedgehog signaling pathway, Wnt signaling pathway
• MW: 39.1 kDa
• Gene Summary: Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity).[UniProtKB/Swiss-Prot Function]