SLC35E3 (NM_018656) Human Tagged ORF Clone

CAT#: RC205831

SLC35E3 (Myc-DDK-tagged)-Human solute carrier family 35, member E3 (SLC35E3)

Product Images

Circular map for RC205831

• 

  Western blot validation of overexpression lysate (Cat# LY412963) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205831 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC35E3
• Synonyms: BLOV1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205831 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATTGCTGGTGGACCGAGTGCGGGGCCACTGGCGAATCGCCGCCGGGCTCCTGTTCAACCTGCTGG
  TGTCCATCTGCATTGTGTTCCTCAACAAATGGATTTATGTGTACCACGGCTTCCCCAACATGAGCCTGAC
  CCTGGTGCACTTCGTGGTCACCTGGCTGGGCTTGTATATCTGCCAGAAGCTGGACATCTTTGCCCCCAAA
  AGTCTGCCGCCCTCCAGGCTCCTCCTCCTGGCCCTCAGCTTCTGTGGCTTTGTGGTCTTCACTAACCTTT
  CTCTGCAGAACAACACCATAGGCACCTATCAGCTGGCCAAGGCCATGACCACGCCGGTGATCATAGCCAT
  CCAGACCTTCTGCTACCAGAAAACCTTCTCCACCAGAATCCAGCTCACGCTGATTCCTATAACTTTAGGT
  GTAATCCTAAATTCTTATTACGATGTGAAGTTTAATTTCCTTGGAATGGTGTTTGCTGCTCTTGGTGTTT
  TAGTTACATCCCTTTATCAAGTGTGGGTAGGAGCCAAACAGCATGAATTACAAGTGAACTCAATGCAGCT
  GCTGTACTACCAGGCTCCGATGTCATCTGCCATGTTGCTGGTTGCTGTGCCCTTCTTTGAGCCAGTGTTT
  GGAGAAGGAGGAATATTTGGTCCCTGGTCAGTTTCTGCTTTGCTTATGGTGCTGCTATCTGGAGTAATAG
  CTTTCATGGTGAACTTATCAATTTATTGGATCATTGGGAACACTTCACCTGTCACCTATAACATGTTCGG
  ACACTTCAAGTTCTGCATTACTTTATTCGGAGGATATGTTTTATTTAAGGATCCACTGTCCATTAATCAG
  GCCCTTGGCATTTTATGTACATTATTTGGCATTCTCGCCTATACCCACTTTAAGCTCAGTGAACAGGAAG
  GAAGTAGGAGTAAACTGGCACAACGTCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205831 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALLVDRVRGHWRIAAGLLFNLLVSICIVFLNKWIYVYHGFPNMSLTLVHFVVTWLGLYICQKLDIFAPK
  SLPPSRLLLLALSFCGFVVFTNLSLQNNTIGTYQLAKAMTTPVIIAIQTFCYQKTFSTRIQLTLIPITLG
  VILNSYYDVKFNFLGMVFAALGVLVTSLYQVWVGAKQHELQVNSMQLLYYQAPMSSAMLLVAVPFFEPVF
  GEGGIFGPWSVSALLMVLLSGVIAFMVNLSIYWIIGNTSPVTYNMFGHFKFCITLFGGYVLFKDPLSINQ
  ALGILCTLFGILAYTHFKLSEQEGSRSKLAQRP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018656
• ORF Size: 939 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018656.5
• RefSeq Size: 2347 bp
• RefSeq ORF: 942 bp
• Locus ID: 55508
• UniProt ID: Q7Z769
• Cytogenetics: 12q15
• Protein Families: Transmembrane
• MW: 35.1 kDa
• Gene Summary: Putative transporter.[UniProtKB/Swiss-Prot Function]