TRAP80 (MED17) (NM_004268) Human Tagged ORF Clone

CAT#: RC205823

MED17 (Myc-DDK-tagged)-Human mediator complex subunit 17 (MED17)

Product Images

Circular map for RC205823

• 

  Western blot validation of overexpression lysate (Cat# LY401377) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205823 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRAP80
• Synonyms: CRSP6; CRSP77; DRIP80; SRB4; TRAP80
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205823 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGGGGTGCGCGCAGTGCGGATCAGCATCGAATCGGCCTGCGAGAAGCAGGTCCATGAGGTGGGCC
  TGGATGGCACCGAGACGTACCTGCCCCCGCTGTCCATGTCGCAGAATCTGGCGCGTCTGGCCCAGCGGAT
  AGACTTCAGCCAGGGTTCGGGCTCCGAGGAGGAGGAGGCGGCGGGGACCGAGGGGGACGCGCAGGACTGG
  CCGGGCGCCGGGTCCAGCGCAGACCAGGACGACGAGGAAGGAGTGGTAAAATTTCAGCCTTCCCTTTGGC
  CTTGGGACTCAGTGAGGAACAATTTGAGAAGTGCCCTGACAGAGATGTGTGTTCTCTATGATGTTCTCAG
  TATTGTTAGGGATAAAAAATTTATGACTCTTGATCCTGTCTCTCAGGATGCACTTCCTCCAAAACAGAAT
  CCTCAGACGTTGCAATTGATATCTAAAAAGAAGTCACTTGCTGGAGCAGCACAAATCTTATTGAAGGGGG
  CAGAAAGACTGACTAAATCAGTTACCGAAAACCAAGAAAACAAGCTACAAAGAGACTTCAATTCTGAGCT
  TTTGCGATTACGGCAACACTGGAAACTTCGAAAAGTTGGAGATAAAATTCTCGGAGATCTGAGCTACAGA
  AGTGCAGGATCTCTCTTTCCTCATCATGGTACATTTGAAGTAATAAAGAATACAGATCTCGATCTGGATA
  AAAAGATACCTGAAGATTACTGTCCTCTTGATGTCCAAATTCCTAGTGATTTAGAGGGGTCTGCATATAT
  CAAGGTTTCAATACAAAAACAGGCTCCAGATATAGGTGACCTCGGCACAGTTAACCTCTTCAAACGACCT
  TTGCCCAAATCCAAACCAGGTTCCCCACATTGGCAGACAAAATTAGAAGCGGCACAGAATGTTCTCTTAT
  GTAAAGAAATTTTTGCACAGCTCTCTCGGGAAGCTGTTCAAATTAAATCACAAGTCCCTCACATTGTGGT
  GAAAAACCAGATTATCTCTCAGCCCTTTCCGAGCTTGCAGTTATCTATTTCTTTGTGCCATTCCTCAAAT
  GATAAGAAATCCCAAAAATTTGCTACTGAGAAGCAATGTCCGGAGGACCACCTTTATGTCCTAGAGCATA
  ATTTGCATCTACTGATTAGAGAGTTTCATAAACAGACCTTGAGTTCCATCATGATGCCTCATCCAGCAAG
  TGCACCTTTTGGCCACAAGAGAATGAGACTTTCGGGTCCTCAAGCTTTTGATAAAAATGAAATTAATTCA
  TTACAGTCCAGTGAAGGGCTTCTGGAAAAAATAATTAAACAAGCAAAGCATATTTTTCTAAGGAGTAGAG
  CTGCTGCAACCATTGACAGCTTAGCAAGCCGAATTGAGGATCCTCAGATACAGGCTCATTGGTCAAATAT
  CAATGATGTTTATGAATCTAGTGTGAAAGTTTTAATCACATCACAAGGCTATGAACAAATATGCAAGTCC
  ATTCAACTGCAATTGAATATTGGAGTTGAGCAGATTCGAGTTGTACATAGAGATGGAAGAGTAATTACAC
  TGTCTTATCAGGAGCAGGAGCTACAGGATTTTCTTCTGTCTCAGATGTCACAGCACCAGGTACATGCAGT
  TCAGCAACTCGCCAAGGTTATGGGCTGGCAAGTACTGAGCTTCAGTAATCATGTGGGACTTGGACCTATA
  GAGAGCATTGGTAATGCATCTGCCATCACGGTGGCCTCCCCAAGTGGTGACTATGCTATTTCAGTTCGTA
  ATGGACCTGAAAGTGGCAGCAAGATTATGGTTCAGTTTCCTCGTAACCAATGTAAAGACCTTCCAAAAAG
  TGATGTTTTACAAGATAACAAATGGAGTCATCTTCGTGGGCCATTCAAAGAAGTTCAGTGGAATAAAATG
  GAAGGTCGAAATTTTGTTTATAAAATGGAGCTGCTTATGTCTGCACTTAGCCCTTGTCTACTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205823 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSGVRAVRISIESACEKQVHEVGLDGTETYLPPLSMSQNLARLAQRIDFSQGSGSEEEEAAGTEGDAQDW
  PGAGSSADQDDEEGVVKFQPSLWPWDSVRNNLRSALTEMCVLYDVLSIVRDKKFMTLDPVSQDALPPKQN
  PQTLQLISKKKSLAGAAQILLKGAERLTKSVTENQENKLQRDFNSELLRLRQHWKLRKVGDKILGDLSYR
  SAGSLFPHHGTFEVIKNTDLDLDKKIPEDYCPLDVQIPSDLEGSAYIKVSIQKQAPDIGDLGTVNLFKRP
  LPKSKPGSPHWQTKLEAAQNVLLCKEIFAQLSREAVQIKSQVPHIVVKNQIISQPFPSLQLSISLCHSSN
  DKKSQKFATEKQCPEDHLYVLEHNLHLLIREFHKQTLSSIMMPHPASAPFGHKRMRLSGPQAFDKNEINS
  LQSSEGLLEKIIKQAKHIFLRSRAAATIDSLASRIEDPQIQAHWSNINDVYESSVKVLITSQGYEQICKS
  IQLQLNIGVEQIRVVHRDGRVITLSYQEQELQDFLLSQMSQHQVHAVQQLAKVMGWQVLSFSNHVGLGPI
  ESIGNASAITVASPSGDYAISVRNGPESGSKIMVQFPRNQCKDLPKSDVLQDNKWSHLRGPFKEVQWNKM
  EGRNFVYKMELLMSALSPCLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004268
• ORF Size: 1953 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004268.5
• RefSeq Size: 3497 bp
• RefSeq ORF: 1956 bp
• Locus ID: 9440
• UniProt ID: Q9NVC6
• Cytogenetics: 11q21
• Protein Families: Druggable Genome, Transcription Factors
• MW: 72.9 kDa
• Gene Summary: The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. [provided by RefSeq, Jul 2008]