PATZ1 (NM_032051) Human Tagged ORF Clone

CAT#: RC205802

PATZ1 (Myc-DDK-tagged)-Human POZ (BTB) and AT hook containing zinc finger 1 (PATZ1), transcript variant 4

Product Images

Circular map for RC205802

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PATZ1 (Cat# RC205802, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PATZ1 (Cat# TA809258)(1:2000). Positive lysates LY410365 (100ug) and LC410365 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY410365) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205802 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PATZ1
• Synonyms: dJ400N23; MAZR; PATZ; RIAZ; ZBTB19; ZNF278; ZSG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205802 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGGTGAACGACGCTTCGTGCGGCCCGTCTGGCTGCTACACATACCAGGTGAGCAGACACAGCA
  CGGAGATGCTGCACAACCTGAACCAGCAGCGCAAAAACGGCGGGCGCTTCTGCGACGTGCTCTTGCGGGT
  AGGCGACGAGAGCTTCCCAGCGCACCGCGCCGTGCTGGCCGCCTGCAGCGAGTACTTTGAGTCGGTGTTC
  AGCGCCCAGTTGGGCGACGGCGGAGCTGCGGACGGGGGTCCGGCTGATGTAGGGGGCGCGACGGCAGCAC
  CAGGCGGCGGGGCCGGGGGCAGCCGGGAGCTGGAGATGCACACTATCAGCTCCAAGGTATTTGGGGACAT
  TCTGGACTTCGCCTACACTTCCCGCATCGTGGTGCGCTTGGAGAGCTTTCCCGAACTCATGACGGCCGCC
  AAGTTCCTGCTGATGAGGTCGGTTATCGAGATCTGCCAGGAAGTCATCAAACAGTCCAACGTACAGATCC
  TGGTACCCCCTGCCCGCGCCGATATAATGCTCTTTCGCCCCCCTGGGACCTCGGACTTGGGCTTCCCTTT
  GGACATGACCAACGGGGCAGCCTTGGCAGCCAACAGCAATGGCATCGCCGGCAGCATGCAGCCAGAGGAG
  GAGGCAGCTCGGGCGGCTGGTGCAGCCATTGCAGGCCAAGCCTCTTTGCCTGTGTTACCTGGGGTGGACC
  GCTTGCCCATGGTGGCTGGACCCCTATCCCCCCAACTGCTGACTTCCCCATTCCCCAGTGTGGCATCCAG
  TGCCCCTCCCCTGACTGGCAAGCGAGGCCGGGGCCGCCCAAGGAAGGCCAACCTGCTGGACTCAATGTTT
  GGGTCCCCAGGGGGCCTGAGGGAGGCAGGCATCCTTCCATGCGGTCTATGTGGTAAGGTGTTCACTGATG
  CCAACCGGCTCCGGCAGCACGAGGCCCAGCACGGTGTCACCAGCCTCCAGCTGGGCTACATCGACCTTCC
  TCCTCCGAGGCTGGGTGAGAATGGGCTACCCATCTCTGAAGACCCCGACGGCCCCCGAAAGAGGAGCCGG
  ACCAGGAAGCAGGTGGCTTGTGAGATCTGCGGCAAGATCTTCCGTGATGTGTATCATCTTAACCGGCACA
  AGCTGTCCCACTCTGGGGAGAAGCCCTACTCCTGCCCTGTGTGTGGGTTGCGGTTCAAGAGAAAAGACCG
  CATGTCCTACCATGTGCGGTCCCATGATGGGTCCGTGGGCAAGCCTTACATCTGCCAGAGCTGTGGGAAA
  GGCTTCTCCAGGCCTGATCACTTGAACGGACATATCAAGCAGGTGCACACTTCTGAGCGGCCTCACAAGT
  GTCAGGTGTGGGTTGGGAGCAGCAGCGGCCTGCCGCCCCTGGAACCTCTTCCTAGCGACCTGCCATCATG
  GGACTTTGCCCAGCCTGCTTTGTGGAGGTCGTCCCATTCGGTTCCTGACACCGCCTTTTCCCTTTCTCTA
  AAAAAATCATTCCCAGCCCTTGAAAACCTGGGCCCAGCACACTCCAGCAACACTCTCTTCTGCCCAGCCC
  CGCCGGGATATCTGAGGCAGGGCTGGACCACCCCAGAGGGCAGCAGGGCCTTTACCCAGTGGCCTGTTGG
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205802 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MERVNDASCGPSGCYTYQVSRHSTEMLHNLNQQRKNGGRFCDVLLRVGDESFPAHRAVLAACSEYFESVF
  SAQLGDGGAADGGPADVGGATAAPGGGAGGSRELEMHTISSKVFGDILDFAYTSRIVVRLESFPELMTAA
  KFLLMRSVIEICQEVIKQSNVQILVPPARADIMLFRPPGTSDLGFPLDMTNGAALAANSNGIAGSMQPEE
  EAARAAGAAIAGQASLPVLPGVDRLPMVAGPLSPQLLTSPFPSVASSAPPLTGKRGRGRPRKANLLDSMF
  GSPGGLREAGILPCGLCGKVFTDANRLRQHEAQHGVTSLQLGYIDLPPPRLGENGLPISEDPDGPRKRSR
  TRKQVACEICGKIFRDVYHLNRHKLSHSGEKPYSCPVCGLRFKRKDRMSYHVRSHDGSVGKPYICQSCGK
  GFSRPDHLNGHIKQVHTSERPHKCQVWVGSSSGLPPLEPLPSDLPSWDFAQPALWRSSHSVPDTAFSLSL
  KKSFPALENLGPAHSSNTLFCPAPPGYLRQGWTTPEGSRAFTQWPVG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032051
• ORF Size: 1611 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_032051.2
• RefSeq Size: 3021 bp
• RefSeq ORF: 1614 bp
• Locus ID: 23598
• UniProt ID: Q9HBE1
• Cytogenetics: 22q12.2
• Domains: BTB, AT_hook, zf-C2H2
• Protein Families: Transcription Factors
• MW: 57.6 kDa
• Gene Summary: The protein encoded by this gene contains an A-T hook DNA binding motif which usually binds to other DNA binding structures to play an important role in chromatin modeling and transcription regulation. Its Poz domain is thought to function as a site for protein-protein interaction and is required for transcriptional repression, and the zinc-fingers comprise the DNA binding domain. Since the encoded protein has typical features of a transcription factor, it is postulated to be a repressor of gene expression. In small round cell sarcoma, this gene is fused to EWS by a small inversion of 22q, then the hybrid is thought to be translocated (t(1;22)(p36.1;q12). The rearrangement of chromosome 22 involves intron 8 of EWS and exon 1 of this gene creating a chimeric sequence containing the transactivation domain of EWS fused to zinc finger domain of this protein. This is a distinct example of an intra-chromosomal rearrangement of chromosome 22. Four alternatively spliced transcript variants are described for this gene. [provided by RefSeq, Jul 2008]