ZNF184 (NM_007149) Human Tagged ORF Clone

CAT#: RC205757

ZNF184 (Myc-DDK-tagged)-Human zinc finger protein 184 (ZNF184)

Product Images

Circular map for RC205757

• 

  Western blot validation of overexpression lysate (Cat# LY416155) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205757 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF184
• Synonyms: kr-ZNF3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205757 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGATCTGTCCTCTCCAGACTCCACCCTTCTCCAAGGGGGACATAATCTACTCTCATCAGCCAGTT
  TTCAGGAATCGGTGACTTTCAAGGATGTGATAGTGGACTTTACCCAGGAAGAATGGAAACAGCTGGACCC
  TGGCCAGAGAGATTTGTTCAGGGATGTGACATTGGAAAATTATACACACCTGGTCTCTATAGGACTCCAA
  GTTTCTAAACCTGATGTGATTTCCCAGTTAGAGCAAGGGACAGAGCCATGGATCATGGAGCCAAGCATTC
  CAGTAGGTACCTGTGCAGACTGGGAGACAAGACTTGAAAATAGTGTGTCAGCCCCAGAGCCTGACATTTC
  TGAAGAAGAGCTATCTCCAGAGGTAATAGTGGAAAAACACAAAAGAGATGATTCTTGGAGTTCCAACTTG
  CTAGAAAGTTGGGAATATGAAGGCAGTTTAGAGAGACAGCAGGCAAACCAACAGACACTGCCAAAGGAAA
  TAAAGGTAACCGAAAAGACAATACCCAGTTGGGAAAAAGGCCCTGTAAATAATGAATTTGGGAAAAGTGT
  CAATGTGAGTTCAAACCTTGTAACACAAGAACCATCTCCAGAAGAGACCTCTACTAAAAGAAGCATCAAA
  CAGAATTCAAACCCAGTTAAAAAAGAGAAATCTTGTAAGTGCAATGAATGTGGGAAAGCCTTTAGTTATT
  GTTCAGCTCTTATTCGCCATCAGAGAACACATACTGGAGAAAAACCCTACAAATGTAATGAATGTGAAAA
  AGCCTTCAGCCGGAGTGAAAACCTTATAAACCATCAAAGAATTCATACTGGAGATAAACCATATAAATGT
  GATCAGTGTGGAAAAGGCTTCATTGAGGGTCCATCTCTTACTCAACATCAAAGAATTCATACTGGAGAAA
  AACCATATAAATGTGATGAATGTGGGAAAGCCTTTAGTCAGAGGACCCATCTTGTTCAGCATCAGAGAAT
  TCATACTGGCGAGAAGCCATACACTTGTAATGAGTGTGGAAAAGCCTTTAGCCAGAGAGGCCACTTTATG
  GAACATCAGAAAATTCATACGGGAGAAAAACCTTTTAAATGTGATGAATGTGATAAAACCTTCACCAGGA
  GCACACACCTTACTCAACATCAAAAAATTCATACTGGAGAAAAAACCTATAAATGTAATGAATGTGGAAA
  GGCCTTCAACGGGCCCTCAACTTTTATCCGTCATCATATGATTCATACTGGTGAAAAACCGTACGAATGC
  AATGAATGTGGGAAAGCCTTCAGCCAGCACTCAAACCTCACTCAGCATCAAAAAACTCATACTGGGGAGA
  AACCCTATGATTGTGCAGAATGTGGAAAATCTTTTAGTTACTGGTCATCCCTTGCTCAACACCTGAAAAT
  TCATACTGGAGAAAAACCTTACAAATGCAATGAATGTGGAAAGGCCTTCAGTTACTGCTCATCCCTTACT
  CAACATCGGAGAATTCACACGAGAGAAAAGCCCTTTGAATGCAGTGAATGTGGAAAGGCTTTCAGTTATC
  TCTCAAACCTTAATCAGCATCAGAAAACTCATACTCAAGAGAAAGCTTATGAATGTAAAGAATGTGGGAA
  AGCTTTTATTCGGAGTTCATCTCTTGCTAAGCATGAAAGAATTCATACTGGAGAGAAACCCTATCAGTGT
  CATGAATGTGGGAAAACCTTCAGTTATGGTTCATCCCTTATTCAGCATAGGAAGATCCATACTGGAGAAC
  GACCTTACAAGTGTAATGAGTGTGGGAGAGCATTCAACCAGAACATACACCTTACACAGCATAAGAGAAT
  TCATACAGGAGCCAAGCCTTATGAGTGTGCTGAGTGTGGTAAAGCCTTTCGACATTGTTCATCTCTTGCT
  CAACATCAAAAAACTCACACAGAAGAAAAACCCTACCAGTGTAATAAATGTGAAAAGACCTTTAGCCAGA
  GCTCCCATCTAACTCAGCATCAACGAATTCACACTGGGGAGAAGCCCTATAAGTGCAATGAATGTGACAA
  AGCCTTTAGCCGGAGCACTCATCTGACTGAACATCAGAATACTCATACTGGAGAGAAACCTTATAACTGT
  AATGAATGCAGAAAGACTTTTAGCCAGAGCACATATCTCATTCAGCACCAGAGAATTCATTCAGGAGAGA
  AGCCTTTTGGATGTAATGATTGTGGAAAATCCTTCAGATATCGCTCTGCTCTCAACAAACATCAGAGACT
  GCATCCTGGCATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205757 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEDLSSPDSTLLQGGHNLLSSASFQESVTFKDVIVDFTQEEWKQLDPGQRDLFRDVTLENYTHLVSIGLQ
  VSKPDVISQLEQGTEPWIMEPSIPVGTCADWETRLENSVSAPEPDISEEELSPEVIVEKHKRDDSWSSNL
  LESWEYEGSLERQQANQQTLPKEIKVTEKTIPSWEKGPVNNEFGKSVNVSSNLVTQEPSPEETSTKRSIK
  QNSNPVKKEKSCKCNECGKAFSYCSALIRHQRTHTGEKPYKCNECEKAFSRSENLINHQRIHTGDKPYKC
  DQCGKGFIEGPSLTQHQRIHTGEKPYKCDECGKAFSQRTHLVQHQRIHTGEKPYTCNECGKAFSQRGHFM
  EHQKIHTGEKPFKCDECDKTFTRSTHLTQHQKIHTGEKTYKCNECGKAFNGPSTFIRHHMIHTGEKPYEC
  NECGKAFSQHSNLTQHQKTHTGEKPYDCAECGKSFSYWSSLAQHLKIHTGEKPYKCNECGKAFSYCSSLT
  QHRRIHTREKPFECSECGKAFSYLSNLNQHQKTHTQEKAYECKECGKAFIRSSSLAKHERIHTGEKPYQC
  HECGKTFSYGSSLIQHRKIHTGERPYKCNECGRAFNQNIHLTQHKRIHTGAKPYECAECGKAFRHCSSLA
  QHQKTHTEEKPYQCNKCEKTFSQSSHLTQHQRIHTGEKPYKCNECDKAFSRSTHLTEHQNTHTGEKPYNC
  NECRKTFSQSTYLIQHQRIHSGEKPFGCNDCGKSFRYRSALNKHQRLHPGI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007149
• ORF Size: 2253 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007149.1, NP_009080.1
• RefSeq Size: 3119 bp
• RefSeq ORF: 2256 bp
• Locus ID: 7738
• UniProt ID: Q99676
• Cytogenetics: 6p22.1
• Domains: KRAB, zf-C2H2
• MW: 86.2 kDa
• Gene Summary: The protein encoded by this gene is predicted to be a Kruppel C2H2-type zinc-finger protein family member. Sequence analysis predicts that the protein contains two Kruppel associated box (KRAB) boxes in the N-terminus and highly conserved zinc finger motifs at the C-terminus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2016]