YJU2 (NM_018074) Human Tagged ORF Clone

CAT#: RC205711

CCDC94 (Myc-DDK-tagged)-Human coiled-coil domain containing 94 (CCDC94)

Product Images

Circular map for RC205711

• 

  Western blot validation of overexpression lysate (Cat# LY413333) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205711 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified YJU2 protein (Cat# TP305711). The protein was produced from HEK293T cells transfected with YJU2 cDNA clone (Cat# RC205711) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: YJU2
• Synonyms: CCDC94
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205711 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGAGCGAAAAGTATTAAACAAATACTACCCGCCGGACTTTGACCCATCAAAGATCCCCAAACTCA
  AGCTCCCCAAAGACCGGCAGTACGTGGTGCGGCTGATGGCCCCCTTCAACATGAGGTGTAAGACGTGCGG
  AGAATACATCTACAAGGGGAAGAAATTCAATGCTCGGAAGGAGACGGTGCAGAACGAGGTCTACCTGGGC
  CTGCCCATCTTCCGCTTTTACATCAAGTGCACGCGCTGCCTGGCAGAGATCACCTTCAAGACAGACCCTG
  AAAACACAGACTACACCATGGAGCATGGAGCCACGCGGAATTTCCAGGCTGAGAAGCTCCTGGAGGAGGA
  GGAGAAGAGGGTGCAGAAGGAGCGGGAGGACGAGGAGCTGAACAACCCCATGAAGGTGCTGGAGAACCGG
  ACCAAGGACTCCAAGCTGGAGATGGAGGTGCTGGAGAACCTCCAGGAGCTGAAAGACCTGAACCAGCGGC
  AGGCGCACGTGGACTTCGAGGCTATGCTGAGGCAGCACCGCCTGTCGGAGGAGGAGCGGCGGAGGCAGCA
  GCAGGAGGAGGACGAGCAGGAGACCGCGGCCCTGTTGGAGGAAGCCAGAAAGCGAAGACTGCTGGAGGAC
  TCCGACTCAGAGGATGAGGCTGCTCCCTCGCCCCTGCAGCCAGCCCTTCGGCCCAACCCCACCGCCATCC
  TGGATGAGGCCCCAAAGCCCAAGAGGAAGGTGGAGGTCTGGGAGCAGAGCGTTGGCAGCCTGGGCAGCCG
  GCCCCCGCTGTCGAGGCTGGTCGTGGTGAAGAAGGCAAAGGCCGACCCGGACTGCAGCAACGGGCAGCCT
  CAGGCGGCCCCCACCCCAGGAGCCCCGCAGAACAGGAAGGAGGCCAACCCTACACCCCTGACGCCTGGCG
  CGTCCTCCCTGAGCCAACTGGGTGCATACCTGGACAGTGACGACAGCAACGGCAGCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205711 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSERKVLNKYYPPDFDPSKIPKLKLPKDRQYVVRLMAPFNMRCKTCGEYIYKGKKFNARKETVQNEVYLG
  LPIFRFYIKCTRCLAEITFKTDPENTDYTMEHGATRNFQAEKLLEEEEKRVQKEREDEELNNPMKVLENR
  TKDSKLEMEVLENLQELKDLNQRQAHVDFEAMLRQHRLSEEERRRQQQEEDEQETAALLEEARKRRLLED
  SDSEDEAAPSPLQPALRPNPTAILDEAPKPKRKVEVWEQSVGSLGSRPPLSRLVVVKKAKADPDCSNGQP
  QAAPTPGAPQNRKEANPTPLTPGASSLSQLGAYLDSDDSNGSN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018074
• ORF Size: 969 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018074.6
• RefSeq Size: 1441 bp
• RefSeq ORF: 972 bp
• Locus ID: 55702
• UniProt ID: Q9BW85
• Cytogenetics: 19p13.3
• Domains: DUF572
• MW: 37.1 kDa
• Gene Summary: Part of the spliceosome which catalyzes two sequential transesterification reactions, first the excision of the non-coding intron from pre-mRNA and then the ligation of the coding exons to form the mature mRNA (PubMed:29301961). Plays a role in stabilizing the structure of the spliceosome catalytic core and docking of the branch helix into the active site, producing 5'-exon and lariat intron-3'-intermediates (By similarity). May protect cells from TP53-dependent apoptosis upon dsDNA break damage through association with PRP19-CD5L complex (PubMed:22952453).[UniProtKB/Swiss-Prot Function]