GTPBP5 (MTG2) (NM_015666) Human Tagged ORF Clone

CAT#: RC205663

GTPBP5 (Myc-DDK-tagged)-Human GTP binding protein 5 (putative) (GTPBP5)

Product Images

Circular map for RC205663

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  Western blot validation of overexpression lysate (Cat# LY414355) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205663 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MTG2 protein (Cat# TP305663). The protein was produced from HEK293T cells transfected with MTG2 cDNA clone (Cat# RC205663) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GTPBP5
• Synonyms: dJ1005F21.2; GTPBP5; ObgH1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205663 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACCTGCAAGGTGTTTTTCAGCAAGATTGAGGACCGTGTTTCAGGGCGTGGGGCATTGGGCTTTGT
  CCACATGGGCTGGCCTGAAGCCCAGCCGGCTACTGCCACAGCGGGCTTCTCCCAGGCTGCTCTCGGTCGG
  CCGTGCGGACCTCGCCAAGCATCAGGAACTCCCGGGGAAGAAGCTGCTCTCTGAGAAAAAGCTGAAAAGG
  TACTTTGTGGACTATCGGAGAGTGCTTGTCTGTGGAGGAAACGGAGGCGCTGGGGCAAGCTGCTTCCACA
  GTGAGCCCCGCAAGGAGTTTGGAGGCCCTGATGGAGGGGACGGAGGCAACGGTGGACACGTCATTCTGAG
  AGTTGACCAGCAAGTCAAGTCCCTGTCGTCGGTCCTGTCGCGGTACCAGGGTTTCAGTGGAGAAGATGGA
  GGGAGTAAAAACTGCTTCGGGCGCAGTGGCGCCGTCCTCTACATCCGGGTCCCCGTGGGCACGCTGGTGA
  AGGAGGGAGGCAGAGTTGTGGCCGACCTGTCTTGCGTGGGAGATGAGTACATTGCCGCGCTGGGCGGGGC
  AGGAGGGAAAGGCAACCGCTTCTTCCTGGCCAACAACAACCGTGCCCCTGTGACCTGTACCCCTGGACAG
  CCAGGACAGCAGCGAGTTCTCCACCTGGAGCTCAAGACGGTGGCCCACGCCGGAATGGTGGGATTCCCCA
  ACGCCGGGAAGTCCTCACTGCTCCGGGCCATTTCAAACGCCAGACCCGCCGTGGCTTCCTACCCGTTCAC
  CACCCTGAAGCCCCACGTCGGGATCGTCCACTACGAAGGCCACCTACAAATAGCAGTGGCCGACATCCCC
  GGCATCATACGAGGCGCCCACCAGAACAGGGGTCTGGGGTCCGCCTTCCTCAGGCACATCGAGCGCTGCC
  GCTTTCTCTTGTTCGTGGTGGATCTTTCTCAGCCTGAGCCGTGGACTCAAGTTGACGATTTAAAATATGA
  ACTGGAGATGTATGAAAAGGGCCTGTCTGCGAGGCCCCACGCAATCGTCGCAAACAAGATTGACCTCCCT
  GAAGCCCAAGCCAATCTGTCCCAGCTCCGGGATCACTTGGGACAGGAGGTCATCGTGCTGTCGGCGTTGA
  CCGGCGAGAACCTGGAGCAGCTGCTGTTGCACCTGAAGGTGCTGTATGACGCCTACGCGGAGGCCGAGCT
  GGGCCAGGGCCGCCAGCCGCTCAGGTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205663 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPARCFSARLRTVFQGVGHWALSTWAGLKPSRLLPQRASPRLLSVGRADLAKHQELPGKKLLSEKKLKR
  YFVDYRRVLVCGGNGGAGASCFHSEPRKEFGGPDGGDGGNGGHVILRVDQQVKSLSSVLSRYQGFSGEDG
  GSKNCFGRSGAVLYIRVPVGTLVKEGGRVVADLSCVGDEYIAALGGAGGKGNRFFLANNNRAPVTCTPGQ
  PGQQRVLHLELKTVAHAGMVGFPNAGKSSLLRAISNARPAVASYPFTTLKPHVGIVHYEGHLQIAVADIP
  GIIRGAHQNRGLGSAFLRHIERCRFLLFVVDLSQPEPWTQVDDLKYELEMYEKGLSARPHAIVANKIDLP
  EAQANLSQLRDHLGQEVIVLSALTGENLEQLLLHLKVLYDAYAEAELGQGRQPLRW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015666
• ORF Size: 1218 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015666.1
• RefSeq Size: 2949 bp
• RefSeq ORF: 1221 bp
• Locus ID: 26164
• UniProt ID: Q9H4K7
• Cytogenetics: 20q13.33
• Domains: GTP1_OBG
• MW: 44 kDa
• Gene Summary: Small G proteins, such as GTPBP5, act as molecular switches that play crucial roles in the regulation of fundamental cellular processes such as protein synthesis, nuclear transport, membrane trafficking, and signal transduction (Hirano et al., 2006 [PubMed 17054726]).[supplied by OMIM, Mar 2008]