GPR62 (NM_080865) Human Tagged ORF Clone

CAT#: RC205539

GPR62 (Myc-DDK-tagged)-Human G protein-coupled receptor 62 (GPR62)

Product Images

Circular map for RC205539

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPR62
• Synonyms: GPCR8; KPG_005
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205539 representing NM_080865
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAACTCCACAGGGCTGAACGCCTCAGAAGTCGCAGGCTCGTTGGGGTTGATCCTGGCAGCTGTCG
  TGGAGGTGGGGGCACTGCTGGGCAACGGCGCGCTGCTGGTCGTGGTGCTGCGCACGCCGGGACTGCGCGA
  CGCGCTCTACCTGGCGCACCTGTGCGTCGTGGACCTGCTGGCGGCCGCCTCCATCATGCCGCTGGGCCTG
  CTGGCCGCACCGCCGCCCGGGCTGGGCCGCGTGCGCCTGGGCCCCGCGCCATGCCGCGCCGCTCGCTTCC
  TCTCCGCCGCTCTGCTGCCGGCCTGCACGCTCGGGGTGGCCGCACTTGGCCTGGCACGCTACCGCCTCAT
  CGTGCACCCGCTGCGGCCAGGCTCGCGGCCGCCGCCTGTGCTCGTGCTCACCGCCGTGTGGGCCGCGGCG
  GGACTGCTGGGCGCGCTCTCCCTGCTCGGCACGCCGCCCGCACCGCCCCCTGCTCCTGCTCGCTGCTCGG
  TCCTGGCTGGGGGCCTCGGGCCCTTCCGGCCGCTCTGGGCCCTGCTGGCCTTCGCGCTGCCCGCCCTCCT
  GCTGCTCGGCGCCTACGGCGGCATCTTCGTGGTGGCGCGTCGCGCTGCCCTGAGGCCCCCACGGCCGGCG
  CGCGGGTCCCGACTCCACTCGGACTCTCTGGATAGCCGCCTTTCCATCTTGCCGCCGCTCCGGCCTCGCC
  TGCCCGGGGGCAAGGCGGCCCTGGCCCCAGCGCTGGCCGTGGGCCAATTTGCAGCCTGCTGGCTGCCTTA
  TGGCTGCGCGTGCCTGGCGCCCGCAGCGCGGGCCGCGGAAGCCGAAGCGGCTGTCACCTGGGTCGCCTAC
  TCGGCCTTCGCGGCTCACCCCTTCCTGTACGGGCTGCTGCAGCGCCCCGTGCGCTTGGCACTGGGCCGCC
  TCTCTCGCCGTGCACTGCCTGGACCTGTGCGGGCCTGCACTCCGCAAGCCTGGCACCCGCGGGCACTCTT
  GCAATGCCTCCAGAGACCCCCAGAGGGCCCTGCCGTAGGCCCTTCTGAGGCTCCAGAACAGACCCCCGAG
  TTGGCAGGAGGGCGGAGCCCCGCATACCAGGGGCCACCTGAGAGTTCTCTCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205539 representing NM_080865
  Red=Cloning site Green=Tags(s)
  
  MANSTGLNASEVAGSLGLILAAVVEVGALLGNGALLVVVLRTPGLRDALYLAHLCVVDLLAAASIMPLGL
  LAAPPPGLGRVRLGPAPCRAARFLSAALLPACTLGVAALGLARYRLIVHPLRPGSRPPPVLVLTAVWAAA
  GLLGALSLLGTPPAPPPAPARCSVLAGGLGPFRPLWALLAFALPALLLLGAYGGIFVVARRAALRPPRPA
  RGSRLHSDSLDSRLSILPPLRPRLPGGKAALAPALAVGQFAACWLPYGCACLAPAARAAEAEAAVTWVAY
  SAFAAHPFLYGLLQRPVRLALGRLSRRALPGPVRACTPQAWHPRALLQCLQRPPEGPAVGPSEAPEQTPE
  LAGGRSPAYQGPPESSLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080865
• ORF Size: 1104 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080865.4
• RefSeq Size: 2206 bp
• RefSeq ORF: 1107 bp
• Locus ID: 118442
• UniProt ID: Q9BZJ7
• Cytogenetics: 3p21.2
• Protein Families: Druggable Genome, GPCR, Transmembrane
• MW: 37.6 kDa
• Gene Summary: Orphan G-protein coupled receptor. Constitutively activates the G(q/11)/inositol phosphate and the G(s)-alpha/cAMP signaling pathways (PubMed:28827538). Has spontaneous activity for beta-arrestin recruitment (PubMed:28827538). Shows a reciprocal modulation of signaling functions with the melatonin receptor MTNR1B most likely through receptor heteromerization (PubMed:28827538).[UniProtKB/Swiss-Prot Function]