TMEM163 (NM_030923) Human Tagged ORF Clone

CAT#: RC205533

TMEM163 (Myc-DDK-tagged)-Human transmembrane protein 163 (TMEM163)

Product Images

Circular map for RC205533

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  Western blot validation of overexpression lysate (Cat# LY410658) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205533 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TMEM163 protein (Cat# TP305533). The protein was produced from HEK293T cells transfected with TMEM163 cDNA clone (Cat# RC205533) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TMEM163
• Synonyms: DC29; SV31
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205533 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGGCCGCGGGCATCCAGCGCCGCAGCTCCCAGGGGCCCACCGTCCCGCCGCCGCCCCGGGGCC
  ACGCGCCACCGGCTGCCGCCCCCGGCCCGGCCCCGCTGAGCTCCCCGGTGCGCGAGCCGCCCCAGCTGGA
  GGAGGAGCGGCAGGTGCGGATCAGCGAGAGCGGCCAGTTCAGCGACGGGCTGGAGGACCGAGGCTTACTA
  GAAAGCAGCACCCGCCTGAAACCTCACGAAGCCCAGAACTACAGGAAGAAGGCATTGTGGGTGTCCTGGT
  TCTCCATCATTGTCACCCTGGCCCTCGCGGTGGCTGCCTTTACTGTCTCCGTTATGAGGTACAGCGCCTC
  TGCTTTTGGGTTTGCATTTGATGCCATCCTGGACGTCCTGTCATCGGCGATTGTCCTGTGGCGTTACAGC
  AACGCGGCCGCTGTGCACTCTGCCCATAGGGAGTACATAGCCTGTGTCATCTTGGGGGTGATATTCCTTC
  TGTCATCCATATGTATAGTGGTCAAAGCCATCCATGACCTCTCAACTAGGCTGCTCCCAGAAGTGGACGA
  TTTCCTGTTCAGTGTCTCCATTTTAAGTGGGATTCTTTGCAGCATCCTGGCCGTGTTGAAGTTCATGCTG
  GGGAAGGTTCTGACCAGTAGAGCACTCATAACAGATGGGTTTAACTCCCTCGTGGGTGGCGTGATGGGCT
  TCTCCATTCTTCTGAGCGCGGAAGTGTTCAAGCATGACTCAGCGGTCTGGTACCTGGACGGCAGCATAGG
  CGTTCTGATCGGCCTCACCATATTTGCCTATGGGGTCAAACTCCTCATCGACATGGTGCCGAGGGTGAGG
  CAGACACGTCACTACGAGATGTTTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205533 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEPAAGIQRRSSQGPTVPPPPRGHAPPAAAPGPAPLSSPVREPPQLEEERQVRISESGQFSDGLEDRGLL
  ESSTRLKPHEAQNYRKKALWVSWFSIIVTLALAVAAFTVSVMRYSASAFGFAFDAILDVLSSAIVLWRYS
  NAAAVHSAHREYIACVILGVIFLLSSICIVVKAIHDLSTRLLPEVDDFLFSVSILSGILCSILAVLKFML
  GKVLTSRALITDGFNSLVGGVMGFSILLSAEVFKHDSAVWYLDGSIGVLIGLTIFAYGVKLLIDMVPRVR
  QTRHYEMFE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030923
• ORF Size: 867 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030923.3
• RefSeq Size: 1901 bp
• RefSeq ORF: 870 bp
• Locus ID: 81615
• UniProt ID: Q8TC26
• Cytogenetics: 2q21.3
• Protein Families: Transmembrane
• MW: 31.5 kDa
• Gene Summary: May bind zinc and other divalent cations and recruit them to vesicular organelles.[UniProtKB/Swiss-Prot Function]