ADAM32 (NM_145004) Human Tagged ORF Clone

CAT#: RC205515

ADAM32 (Myc-DDK-tagged)-Human ADAM metallopeptidase domain 32 (ADAM32)

Product Images

Circular map for RC205515

• 

  Western blot validation of overexpression lysate (Cat# LY408141) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205515 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ADAM32 protein (Cat# TP305515). The protein was produced from HEK293T cells transfected with ADAM32 cDNA clone (Cat# RC205515) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADAM32
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205515 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCGCCTCTGGTTGCTGCTGGCCGGGCTCTGCGGCCTCCTGGCGTCAAGACCCGGTTTTCAAAATT
  CACTTCTACAGATCGTAATTCCAGAGAAAATCCAAACAAATACAAATGACAGTTCAGAAATAGAATATGA
  ACAAATATCCTATATTATTCCAATAGATGAGAAACTGTACACTGTGCACCTTAAACAAAGATATTTTTTA
  GCAGATAATTTTATGATCTATTTGTACAATCAAGGATCTATGAATACTTATTCTTCAGATATTCAGACTC
  AATGCTACTATCAAGGAAATATTGAAGGATATCCTGATTCCATGGTCACACTCAGCACGTGCTCTGGACT
  AAGAGGAATACTGCAATTTGAAAATGTTTCTTATGGAATTGAGCCTCTGGAATCTGCAGTTGAATTTCAG
  CATGTTCTTTACAAATTAAAGAATGAAGACAATGATATTGCAATTTTTATTGACAGAAGCCTGAAAGAAC
  AACCAATGGATGACAACATTTTTATAAGTGAAAAATCAGAACCAGCTGTTCCAGATTTATTTCCTCTTTA
  TCTAGAAATGCATATTGTGGTGGACAAAACTTTGTATGATTACTGGGGCTCTGATAGCATGATAGTAACA
  AATAAAGTCATCGAAATTGTTGGCCTTGCAAATTCAATGTTCACCCAATTTAAAGTTACTATTGTGCTGT
  CATCATTGGAGTTATGGTCAGATGAAAATAAGATTTCTACAGTTGGTGAGGCAGATGAATTATTGCAAAA
  ATTTTTAGAATGGAAACAATCTTATCTTAACCTAAGGCCTCATGATATTGCATATCTACTAATTTATATG
  GATTATCCTCGTTATTTGGGAGCAGTGTTTCCTGGAACAATGTGTATTACTCGTTATTCTGCAGGAGTTG
  CATTGTACCCCAAGGAGATAACTCTGGAGGCATTTGCAGTTATTGTCACCCAGATGCTGGCACTCAGTCT
  GGGAATATCATATGACGACCCAAAGAAATGTCAATGTTCAGAATCCACCTGTATAATGAATCCAGAAGTT
  GTGCAATCCAATGGTGTGAAGACTTTTAGCAGTTGCAGTTTGAGGAGCTTTCAAAATTTCATTTCAAATG
  TGGGTGTCAAATGTCTTCAGAATAAGCCACAAATGCAAAAAAAATCTCCGAAACCAGTCTGCGGCAATGG
  CAGATTGGAGGGAAATGAAATCTGTGATTGTGGTACTGAGGCTCAATGTGGACCTGCAAGCTGTTGTGAT
  TTTCGAACTTGTGTACTGAAAGACGGAGCAAAATGTTATAAAGGACTGTGCTGCAAAGACTGTCAAATTT
  TACAATCAGGCGTTGAATGTAGGCCGAAAGCACATCCTGAATGTGACATCGCTGAAAATTGTAATGGAAG
  CTCACCAGAATGTGGTCCTGACATAACTTTAATCAATGGACTTTCATGCAAAAATAATAAGTTTATTTGT
  TATGACGGAGACTGCCATGATCTCGATGCACGTTGTGAGAGTGTATTTGGAAAAGGTTCAAGAAATGCTC
  CATTTGCCTGCTATGAAGAAATACAATCTCAATCAGACAGATTTGGGAACTGTGGTAGGGATAGAAATAA
  CAAATATGTGTTCTGTGGATGGAGGAATCTTATATGTGGAAGATTAGTTTGTACATACCCTACTCGAAAG
  CCTTTCCATCAAGAAAATGGTGATGTGATTTATGCTTTCGTACGAGATTCTGTATGCATAACTGTAGACT
  ACAAATTGCCTCGAACAGTTCCAGATCCACTGGCTGTCAAAAATGGCTCTCAGTGTGATATTGGGAGGGT
  TTGTGTAAATCGTGAATGTGTAGAATCAAGGATAATTAAGGCTTCAGCACATGTTTGTTCACAACAGTGT
  TCTGGACATGGAGTGTGTGATTCCAGAAACAAGTGCCATTGTTCGCCAGGCTATAAGCCTCCAAACTGCC
  AAATACGTTCCAAAGGATTTTCCATATTTCCTGAGGAAGATATGGGTTCAATCATGGAAAGAGCATCTGG
  GAAGACTGAAAACACCTGGCTTCTAGGTTTCCTCATTGCTCTTCCTATTCTCATTGTAACAACCGCAATA
  GTTTTGGCAAGGAAACAGTTGAAAAAGTGGTTCGCCAAGGAAGAGGAATTCCCAAGTAGCGAATCTAAAT
  CGGAAGGTAGCACACAGACATATGCCAGCCAATCCAGCTCAGAAGGCAGCACTCAGACATATGCCAGCCA
  AACCAGATCAGAAAGCAGCAGTCAAGCTGATACTAGCAAATCCAAATCAGAAGATAGTGCTGAAGCATAT
  ACTAGCAGATCCAAATCACAGGACAGTACCCAAACACAAAGCAGTAGTAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205515 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFRLWLLLAGLCGLLASRPGFQNSLLQIVIPEKIQTNTNDSSEIEYEQISYIIPIDEKLYTVHLKQRYFL
  ADNFMIYLYNQGSMNTYSSDIQTQCYYQGNIEGYPDSMVTLSTCSGLRGILQFENVSYGIEPLESAVEFQ
  HVLYKLKNEDNDIAIFIDRSLKEQPMDDNIFISEKSEPAVPDLFPLYLEMHIVVDKTLYDYWGSDSMIVT
  NKVIEIVGLANSMFTQFKVTIVLSSLELWSDENKISTVGEADELLQKFLEWKQSYLNLRPHDIAYLLIYM
  DYPRYLGAVFPGTMCITRYSAGVALYPKEITLEAFAVIVTQMLALSLGISYDDPKKCQCSESTCIMNPEV
  VQSNGVKTFSSCSLRSFQNFISNVGVKCLQNKPQMQKKSPKPVCGNGRLEGNEICDCGTEAQCGPASCCD
  FRTCVLKDGAKCYKGLCCKDCQILQSGVECRPKAHPECDIAENCNGSSPECGPDITLINGLSCKNNKFIC
  YDGDCHDLDARCESVFGKGSRNAPFACYEEIQSQSDRFGNCGRDRNNKYVFCGWRNLICGRLVCTYPTRK
  PFHQENGDVIYAFVRDSVCITVDYKLPRTVPDPLAVKNGSQCDIGRVCVNRECVESRIIKASAHVCSQQC
  SGHGVCDSRNKCHCSPGYKPPNCQIRSKGFSIFPEEDMGSIMERASGKTENTWLLGFLIALPILIVTTAI
  VLARKQLKKWFAKEEEFPSSESKSEGSTQTYASQSSSEGSTQTYASQTRSESSSQADTSKSKSEDSAEAY
  TSRSKSQDSTQTQSSSN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145004
• ORF Size: 2361 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145004.3
• RefSeq Size: 2742 bp
• RefSeq ORF: 2364 bp
• Locus ID: 203102
• UniProt ID: Q8TC27
• Cytogenetics: 8p11.22
• Protein Families: Protease, Transmembrane
• MW: 87.9 kDa
• Gene Summary: This gene encodes a member of the disintegrin family of membrane-anchored proteins that play a role in diverse biological processes such as brain development, fertilization, tumor development and inflammation. This gene is predominantly expressed in the testis. The encoded protein undergoes proteolytic processing to generate a mature polypeptide comprised of an metalloprotease, disintegrin and epidermal growth factor-like domains. This gene is located in a cluster of other disintegrin and metallopeptidase family genes on chromosome 8. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]