SPAG6 (NM_172242) Human Tagged ORF Clone

CAT#: RC205275

SPAG6 (Myc-DDK-tagged)-Human sperm associated antigen 6 (SPAG6), transcript variant 2

Product Images

Circular map for RC205275

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  Western blot validation of overexpression lysate (Cat# LY406761) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205275 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SPAG6 protein (Cat# TP305275). The protein was produced from HEK293T cells transfected with SPAG6 cDNA clone (Cat# RC205275) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SPAG6
• Synonyms: CFAP194; CT141; FAP194; pf16; Repro-SA-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205275 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCAGAGGCAGGTGCTGCAAGTGTTCGAGCAATACCAGAAGGCCAGGACCCAGTTCGTGCAGATGG
  TGGCGGAGCTGGCGACTAGACCCCAAAACATCGAGACGCTGCAGAACGCGGGTGTAATGTCTTTGCTGAG
  AACTCTTCTTCTGGACGTGGTCCCAACAATTCAACAGATTGCTGCTTTGGCTCTTGGGAGACTGGCCAAT
  TATAATGATGACCTAGCAGAAGCTGTTGTGAAGTGCGACATTCTTCCACAGCTTGTTTATTCATTGGCAG
  AACAGAATCGCTTCTACAAGAAAGCAGCTGCCTTTGTGTTACGAGCAGTTGGTAAACATTCTCCCCAGCT
  AGCTCAGGCAATAGTCGATTGTGGAGCACTGGATACGCTGGTCATATGCTTGGAAGATTTTGACCCTGGA
  GTCAAGGAGGCTGCAGCCTGGGCACTTAGATATATTGCAAGACATAATGCAGAACTGTCACAAGCTGTGG
  TGGATGCAGGAGCTGTTCCTCTTTTAGTACTCTGTATCCAGGAGCCAGAAATTGCTTTGAAAGGGATTGC
  TGCTTCGGCCCTCAGTGATATTGTAAAGCATTCTCCAGAGTTAGCACAGACAGTAGTGGATGCAGGAGCT
  GTTGCTCATTTAGCCCAGATGATCCTGAACCCTGATGCTAAATTGAAGCATCAGATCCTTTCAGCTCTCA
  GTCAGGTTTCAAAACATTCCGTGGATCTGGCAGAAATGGTTGTTGAAGCAGAGATTTTTCCAGTTGTACT
  TACCTGTCTGAAGGACAAGGATGAATACGTGAAGAAAAATGCTTCTACTTTAATTAGAGAGATTGCAAAA
  CATACACCCGAGCTTTCACAGCTGGTAGTTAACGCAGGAGGGGTTGCTGCCGTGATTGACTGCATTGGGT
  CCTGCAAAGGGAACACACGGCTGCCTGGCATCATGATGCTTGGTTATGTAGCAGCTCATTCTGAGAACCT
  AGCAATGGCAGTCATCATTTCTAAGGGTGTACCCCAGTTGTCAGTCTGCTTGTCAGAAGAACCGGAAGAT
  CATATTAAGGCTGCAGCTGCTTGGGCCTTAGGACAGATTGGAAGACACACTCCTGAACACGCACGGGCTG
  TTGCAGTCACAAATACTTTGCCAGTTCTGCTTTCTTTGTACATGTCAACAGAAAGTTCTGAGGATCTCCA
  AGTAAAAAGTAAAAAAGCCATAAAGAATATCCTGCAAAAATGTACCTACTTACCAGCCCTTGAACCATTT
  CTATATGATGCTCCTCCCAATATTCTGAAACATGTGGTTGGACAGTTCAGTAAGGTATTATTCCCCTGGA
  TATTCAGATACACTTCTGCGGAGGGTGGACAGCTATCAACCACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205275 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSQRQVLQVFEQYQKARTQFVQMVAELATRPQNIETLQNAGVMSLLRTLLLDVVPTIQQIAALALGRLAN
  YNDDLAEAVVKCDILPQLVYSLAEQNRFYKKAAAFVLRAVGKHSPQLAQAIVDCGALDTLVICLEDFDPG
  VKEAAAWALRYIARHNAELSQAVVDAGAVPLLVLCIQEPEIALKGIAASALSDIVKHSPELAQTVVDAGA
  VAHLAQMILNPDAKLKHQILSALSQVSKHSVDLAEMVVEAEIFPVVLTCLKDKDEYVKKNASTLIREIAK
  HTPELSQLVVNAGGVAAVIDCIGSCKGNTRLPGIMMLGYVAAHSENLAMAVIISKGVPQLSVCLSEEPED
  HIKAAAAWALGQIGRHTPEHARAVAVTNTLPVLLSLYMSTESSEDLQVKSKKAIKNILQKCTYLPALEPF
  LYDAPPNILKHVVGQFSKVLFPWIFRYTSAEGGQLSTT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172242
• ORF Size: 1374 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172242.3
• RefSeq Size: 2488 bp
• RefSeq ORF: 1377 bp
• Locus ID: 9576
• UniProt ID: O75602
• Cytogenetics: 10p12.2
• MW: 49.6 kDa
• Gene Summary: The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from an infertile man. This protein localizes to the tail of permeabilized human sperm and contains eight contiguous armadillo repeats, a motif known to mediate protein-protein interactions. Studies in mice suggest that this protein is involved in sperm flagellar motility and maintenance of the structural integrity of mature sperm. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]