SHC3 (NM_016848) Human Tagged ORF Clone

CAT#: RC205195

SHC3 (Myc-DDK-tagged)-Human SHC (Src homology 2 domain containing) transforming protein 3 (SHC3)

Product Images

Circular map for RC205195

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SHC3
• Synonyms: N-Shc; NSHC; RAI; SHCC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205195 representing NM_016848
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCCACGCACCAAGTATAACCGCTTCAGGAATGACTCGGTGACATCGGTCGATGACCTTCTCCACA
  GCCTGTCGGTGAGCGGCGGCGGAGGCAAGGTTTCGGCGGCGCGCGCGACCCCGGCGGCGGCTCCCTACTT
  GGTGTCCGGCGAGGCGCTGCGCAAGGCGCCCGTCGATGGGCCCGGCAGCCTGGGCCACCTGCTCCACAAG
  GTGTCCCACCTGAAACTCTCCAGCTCGGGCCTCCGCGGCCTGTCGTCGGCCGCCCGGGAGCGGGCGGGCG
  CGCGGCTCTCGGGCAGCTGCAGCGCGCCCAGCCTGGCCGCCCCGGACGGCAGTGCGCCCTCGGCGCCCCG
  CGCCCCGGCCATGAGCGCCGCCAGGAAGGGCCGGCCCGGCGACGAGCCGCTGCCCAGGCCCCCTCGGGGG
  GCGCCGCACGCCAGCGACCAGGTGCTGGGGCCCGGAGTCACCTACGTGGTCAAGTACTTGGGGTGCATTG
  AAGTTCTGCGCTCAATGAGGTCTCTTGACTTCAGTACAAGAACACAAATTACCAGGGAAGCCATCAGCCG
  CGTCTGTGAAGCTGTGCCTGGTGCGAAGGGAGCCTTCAAGAAGAGAAAGCCTCCAAGCAAAATGCTGTCC
  AGCATCTTGGGAAAGAGCAACCTCCAGTTTGCGGGAATGAGCATCTCTCTGACCATCTCCACGGCCAGTC
  TGAACCTGCGAACTCCGGACTCCAAACAGATCATAGCGAATCACCACATGCGGTCCATCTCCTTCGCCTC
  TGGGGGAGACCCGGACACGACTGACTATGTTGCATATGTGACTAAGGACCCTGTTAATCGCAGAGCTTGT
  CACATTTTGGAATGCTGTGATGGGCTGGCCCAGGATGTCATCGGCTCCATCGGACAAGCCTTTGAGCTCC
  GGTTTAAGCAATATTTACAGTGTCCTACCAAGATTCCCGCTCTCCATGATCGAATGCAGAGTCTGGATGA
  GCCATGGACGGAAGAGGAGGGAGATGGCTCAGACCACCCATACTACAACAGCATCCCAAGCAAGATGCCT
  CCTCCAGGGGGCTTTCTTGATACTAGACTGAAACCCAGACCCCATGCTCCTGACACAGCCCAGTTTGCAG
  GAAAAGAGCAGACTTATTACCAGGGAAGACACTTAGGAGACACTTTTGGCGAAGACTGGCAGCAAACACC
  TTTAAGGCAAGGGTCCTCGGACATCTACAGCACGCCAGAAGGGAAACTGCACGTGGCCCCCACGGGAGAA
  GCACCCACCTACGTCAACACTCAGCAGATCCCACCACAGGCCTGGCCGGCTGCGGTCAGCAGTGCTGAGA
  GCAGCCCAAGGAAAGACCTCTTTGACATGAAACCTTTTGAAGATGCTCTCAAGAACCAGCCCTTGGGGCC
  CGTGTTAAGCAAGGCAGCCTCCGTGGAGTGCATCAGCCCTGTGTCACCTAGAGCCCCAGATGCCAAGATG
  CTGGAGGAACTGCAAGCCGAGACTTGGTACCAAGGAGAGATGAGCAGGAAGGAGGCAGAGGGGCTGCTGG
  AGAAAGACGGAGACTTCCTGGTCAGGAAGAGCACCACCAACCCGGGCTCCTTTGTCCTCACGGGCATGCA
  CAATGGCCAGGCCAAGCACCTGCTGCTCGTGGACCCAGAAGGCACGATCCAGACAAAGGACAGAGTCTTT
  GACAGTATCAGCCACCTCATCAACCACCACCTAGAAAGCAGCCTGCCCATTGTCTCTGCAGGGAGTGAGC
  TGTGTCTCCAGCAGCCAGTGGAGAGGAAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205195 representing NM_016848
  Red=Cloning site Green=Tags(s)
  
  MLPRTKYNRFRNDSVTSVDDLLHSLSVSGGGGKVSAARATPAAAPYLVSGEALRKAPVDGPGSLGHLLHK
  VSHLKLSSSGLRGLSSAARERAGARLSGSCSAPSLAAPDGSAPSAPRAPAMSAARKGRPGDEPLPRPPRG
  APHASDQVLGPGVTYVVKYLGCIEVLRSMRSLDFSTRTQITREAISRVCEAVPGAKGAFKKRKPPSKMLS
  SILGKSNLQFAGMSISLTISTASLNLRTPDSKQIIANHHMRSISFASGGDPDTTDYVAYVTKDPVNRRAC
  HILECCDGLAQDVIGSIGQAFELRFKQYLQCPTKIPALHDRMQSLDEPWTEEEGDGSDHPYYNSIPSKMP
  PPGGFLDTRLKPRPHAPDTAQFAGKEQTYYQGRHLGDTFGEDWQQTPLRQGSSDIYSTPEGKLHVAPTGE
  APTYVNTQQIPPQAWPAAVSSAESSPRKDLFDMKPFEDALKNQPLGPVLSKAASVECISPVSPRAPDAKM
  LEELQAETWYQGEMSRKEAEGLLEKDGDFLVRKSTTNPGSFVLTGMHNGQAKHLLLVDPEGTIQTKDRVF
  DSISHLINHHLESSLPIVSAGSELCLQQPVERKQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016848
• ORF Size: 1782 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016848.3
• RefSeq Size: 2400 bp
• RefSeq ORF: 1785 bp
• Locus ID: 53358
• UniProt ID: Q92529
• Cytogenetics: 9q22.1
• Domains: SH2, PID
• Protein Pathways: Chemokine signaling pathway, Chronic myeloid leukemia, ErbB signaling pathway, Focal adhesion, Glioma, Insulin signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway
• MW: 63.9 kDa
• Gene Summary: Signaling adapter that couples activated growth factor receptors to signaling pathway in neurons. Involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons.[UniProtKB/Swiss-Prot Function]