SLC3A1 (NM_000341) Human Tagged ORF Clone

CAT#: RC205049

SLC3A1 (Myc-DDK-tagged)-Human solute carrier family 3 (cystine, dibasic and neutral amino acid transporters, activator of cystine, dibasic and neutral amino acid transport), member 1 (SLC3A

Product Images

Circular map for RC205049

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  Western blot validation of overexpression lysate (Cat# LY424785) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205049 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SLC3A1 protein (Cat# TP305049). The protein was produced from HEK293T cells transfected with SLC3A1 cDNA clone (Cat# RC205049) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC3A1
• Synonyms: ATR1; CSNU1; D2H; NBAT; RBAT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205049 representing NM_000341
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAAGATAAAAGCAAGAGAGACTCCATCGAGATGAGTATGAAGGGATGCCAGACAAACAACGGGT
  TTGTCCATAATGAAGACATTCTGGAGCAGACCCCGGATCCAGGCAGCTCAACAGACAACCTGAAGCACAG
  CACCAGGGGCATCCTTGGCTCCCAGGAGCCCGACTTCAAGGGCGTCCAGCCCTATGCGGGGATGCCCAAG
  GAGGTGCTGTTCCAGTTCTCTGGCCAGGCCCGCTACCGCATACCTCGGGAGATCCTCTTCTGGCTCACAG
  TGGCTTCTGTGCTGGTGCTCATCGCGGCCACCATAGCCATCATTGCCCTCTCTCCAAAGTGCCTAGACTG
  GTGGCAGGAGGGGCCCATGTACCAGATCTACCCAAGGTCTTTCAAGGACAGTAACAAGGATGGGAACGGA
  GATCTGAAAGGTATTCAAGATAAACTGGACTACATCACAGCTTTAAATATAAAAACTGTTTGGATTACTT
  CATTTTATAAATCGTCCCTTAAAGATTTCAGATATGGTGTTGAAGATTTCCGGGAAGTTGATCCCATTTT
  TGGAACGATGGAAGATTTTGAGAATCTGGTTGCAGCCATACATGATAAAGGTTTAAAATTAATCATCGAT
  TTCATACCAAACCACACGAGTGATAAACATATTTGGTTTCAATTGAGTCGGACACGGACAGGAAAATATA
  CTGATTATTATATCTGGCATGACTGTACCCATGAAAATGGCAAAACCATTCCACCCAACAACTGGTTAAG
  TGTGTATGGAAACTCCAGTTGGCACTTTGACGAAGTGCGAAACCAATGTTATTTTCATCAGTTTATGAAA
  GAGCAACCTGATTTAAATTTCCGCAATCCTGATGTTCAAGAAGAAATAAAAGAAATTTTACGGTTCTGGC
  TCACAAAGGGTGTTGATGGTTTTAGTTTGGATGCTGTTAAATTCCTCCTAGAAGCAAAGCACCTGAGAGA
  TGAGATCCAAGTAAATAAGACCCAAATCCCGGACACGGTCACACAATACTCGGAGCTGTACCATGACTTC
  ACCACCACGCAGGTGGGAATGCACGACATTGTCCGCAGCTTCCGGCAGACCATGGACCAATACAGCACGG
  AGCCCGGCAGATACAGGTTCATGGGGACTGAAGCCTATGCAGAGAGTATTGACAGGACCGTGATGTACTA
  TGGATTGCCATTTATCCAAGAAGCTGATTTTCCCTTCAACAATTACCTCAGCATGCTAGACACTGTTTCT
  GGGAACAGCGTGTATGAGGTTATCACATCCTGGATGGAAAACATGCCAGAAGGAAAATGGCCTAACTGGA
  TGATTGGTGGACCAGACAGTTCACGGCTGACTTCGCGTTTGGGGAATCAGTATGTCAACGTGATGAACAT
  GCTTCTTTTCACACTCCCTGGAACTCCTATAACTTACTATGGAGAAGAAATTGGAATGGGAAATATTGTA
  GCCGCAAATCTCAATGAAAGCTATGATATTAATACCCTTCGCTCAAAGTCACCAATGCAGTGGGACAATA
  GTTCAAATGCTGGTTTTTCTGAAGCTAGTAACACCTGGTTACCTACCAATTCAGATTACCACACTGTGAA
  TGTTGATGTCCAAAAGACTCAGCCCAGATCGGCTTTGAAGTTATATCAAGATTTAAGTCTACTTCATGCC
  AATGAGCTACTCCTCAACAGGGGCTGGTTTTGCCATTTGAGGAATGACAGCCACTATGTTGTGTACACAA
  GAGAGCTGGATGGCATCGACAGAATCTTTATCGTGGTTCTGAATTTTGGAGAATCAACACTGTTAAATCT
  ACATAATATGATTTCGGGCCTTCCCGCTAAAATAAGAATAAGGTTAAGTACCAATTCTGCCGACAAAGGC
  AGTAAAGTTGATACAAGTGGCATTTTTCTGGACAAGGGAGAGGGACTCATCTTTGAACACAACACGAAGA
  ATCTCCTTCATCGCCAAACAGCTTTCAGAGATAGATGCTTTGTTTCCAATCGAGCATGCTATTCCAGTGT
  ACTGAACATACTGTATACCTCGTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205049 representing NM_000341
  Red=Cloning site Green=Tags(s)
  
  MAEDKSKRDSIEMSMKGCQTNNGFVHNEDILEQTPDPGSSTDNLKHSTRGILGSQEPDFKGVQPYAGMPK
  EVLFQFSGQARYRIPREILFWLTVASVLVLIAATIAIIALSPKCLDWWQEGPMYQIYPRSFKDSNKDGNG
  DLKGIQDKLDYITALNIKTVWITSFYKSSLKDFRYGVEDFREVDPIFGTMEDFENLVAAIHDKGLKLIID
  FIPNHTSDKHIWFQLSRTRTGKYTDYYIWHDCTHENGKTIPPNNWLSVYGNSSWHFDEVRNQCYFHQFMK
  EQPDLNFRNPDVQEEIKEILRFWLTKGVDGFSLDAVKFLLEAKHLRDEIQVNKTQIPDTVTQYSELYHDF
  TTTQVGMHDIVRSFRQTMDQYSTEPGRYRFMGTEAYAESIDRTVMYYGLPFIQEADFPFNNYLSMLDTVS
  GNSVYEVITSWMENMPEGKWPNWMIGGPDSSRLTSRLGNQYVNVMNMLLFTLPGTPITYYGEEIGMGNIV
  AANLNESYDINTLRSKSPMQWDNSSNAGFSEASNTWLPTNSDYHTVNVDVQKTQPRSALKLYQDLSLLHA
  NELLLNRGWFCHLRNDSHYVVYTRELDGIDRIFIVVLNFGESTLLNLHNMISGLPAKIRIRLSTNSADKG
  SKVDTSGIFLDKGEGLIFEHNTKNLLHRQTAFRDRCFVSNRACYSSVLNILYTSC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000341
• ORF Size: 2055 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000341.2, NP_000332.1
• RefSeq Size: 2323 bp
• RefSeq ORF: 2058 bp
• Locus ID: 6519
• UniProt ID: Q07837
• Cytogenetics: 2p21
• Protein Families: Druggable Genome, Transmembrane
• MW: 78.7 kDa
• Gene Summary: This gene encodes a type II membrane glycoprotein which is one of the components of the renal amino acid transporter which transports neutral and basic amino acids in the renal tubule and intestinal tract. Mutations and deletions in this gene are associated with cystinuria. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]