COG2 (NM_007357) Human Tagged ORF Clone

CAT#: RC204855

COG2 (Myc-DDK-tagged)-Human component of oligomeric golgi complex 2 (COG2), transcript variant 1

Product Images

Circular map for RC204855

• 

  Western blot validation of overexpression lysate (Cat# LY402136) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204855 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified COG2 protein (Cat# TP304855). The protein was produced from HEK293T cells transfected with COG2 cDNA clone (Cat# RC204855) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: COG2
• Synonyms: CDG2Q; LDLC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204855 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAAAGTAGGATGAACCTGCCCAAGGGGCCGGACACGCTCTGCTTCGACAAGGACGAGTTCATGA
  AGGAAGATTTCGATGTCGATCATTTTGTGTCTGACTGTAGGAAGCGGGTCCAGCTGGAAGAACTGAGAGA
  TGACCTGGAGCTCTACTATAAACTTCTTAAAACAGCCATGGTCGAACTCATCAACAAGGATTATGCAGAT
  TTTGTCAATCTTTCAACAAACTTGGTTGGCATGGACAAAGCCCTCAACCAGCTTTCTGTGCCTTTGGGAC
  AATTACGAGAAGAGGTTCTGAGCCTTAGATCGTCTGTCAGTGAAGGAATTCGGGCAGTTGATGAACGAAT
  GTCTAAACAAGAGGACATTAGGAAAAAAAAGATGTGTGTATTGAGGCTTATACAAGTTATTCGGTCAGTT
  GAGAAAATTGAAAAAATCTTAAACTCTCAAAGTTCTAAAGAAACCTCTGCACTAGAAGCAAGCAGCCCCC
  TTTTGACTGGACAAATTTTGGAGAGAATTGCCACAGAATTTAATCAGTTACAGTTTCATGCTGTTCAAAG
  CAAAGGCATGCCTCTTTTGGACAAAGTAAGACCGCGTATAGCTGGCATTACAGCCATGTTACAGCAGTCA
  CTGGAAGGTCTCCTATTAGAAGGCCTTCAGACGTCTGACGTCGATATAATACGGCACTGCTTGCGGACTT
  ACGCCACGATTGACAAGACACGGGACGCGGAGGCCTTAGTTGGCCAAGTACTAGTGAAACCATACATAGA
  CGAGGTGATTATAGAGCAGTTTGTTGAATCTCATCCCAATGGCCTTCAGGTCATGTATAATAAACTCCTG
  GAGTTTGTTCCTCACCATTGCCGCCTTCTTCGAGAAGTCACAGGAGGTGCCATCTCCAGTGAAAAAGGCA
  ATACTGTTCCTGGATATGACTTTTTGGTGAATTCTGTTTGGCCACAAATAGTACAAGGATTAGAAGAAAA
  GTTACCCTCGCTTTTTAATCCTGGGAATCCCGATGCATTTCATGAGAAATATACCATAAGTATGGATTTT
  GTCAGAAGATTGGAACGGCAGTGTGGATCACAGGCTAGTGTAAAGAGATTAAGAGCCCATCCTGCCTATC
  ACAGCTTCAATAAGAAGTGGAACTTGCCTGTTTATTTTCAAATAAGATTTAGAGAAATAGCGGGATCCTT
  AGAAGCAGCACTTACAGATGTCCTGGAAGATGCCCCAGCTGAAAGTCCGTATTGCCTTTTGGCTTCTCAT
  AGAACTTGGAGCAGCCTTAGGAGGTGTTGGTCAGATGAGATGTTCTTGCCATTACTGGTGCATCGCCTGT
  GGAGACTCACTCTGCAGATTTTGGCACGATACTCTGTGTTTGTCAATGAGCTTTCACTCAGGCCCATTTC
  TAATGAAAGTCCCAAGGAGATCAAGAAACCTTTGGTAACTGGTAGCAAAGAACCTTCCATCACCCAAGGA
  AACACTGAAGACCAAGGAAGTGGTCCTTCGGAAACAAAGCCTGTGGTTTCCATTTCCCGCACTCAGCTCG
  TGTATGTGGTTGCAGACCTGGACAAGCTTCAGGAGCAGCTTCCAGAACTCTTGGAAATAATCAAGCCAAA
  ACTTGAAATGATTGGCTTTAAGAATTTTTCTTCTATCTCAGCAGCCCTGGAGGACTCCCAGAGCTCTTTT
  TCAGCCTGTGTGCCCTCCTTGAGTAGCAAGATCATCCAGGATTTAAGTGACTCTTGCTTCGGTTTCCTAA
  AAAGCGCCCTGGAGGTTCCCAGGCTTTACCGAAGAACCAATAAGGAGGTCCCAACCACAGCTTCCTCCTA
  TGTGGACAGTGCTCTGAAGCCCTTATTCCAGCTTCAGAGCGGACACAAGGATAAGCTCAAACAAGCAATA
  ATTCAGCAGTGGCTAGAAGGCACTCTCAGTGAAAGCACTCATAAGTACTATGAAACCGTGTCAGATGTAT
  TAAACTCTGTGAAGAAGATGGAAGAGAGCCTGAAAAGGCTGAAACAAGCCAGAAAAACCACTCCCGCCAA
  CCCCGTCGGTCCCAGTGGTGGCATGAGCGACGACGACAAAATCAGGCTGCAGTTGGCCCTAGATGTTGAG
  TACTTGGGAGAGCAGATACAAAAGTTGGGACTACAAGCAAGTGACATAAAAAGCTTCTCAGCTCTCGCAG
  AGCTTGTTGCTGCTGCCAAGGACCAGGCAACAGCAGAGCAGCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204855 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEKSRMNLPKGPDTLCFDKDEFMKEDFDVDHFVSDCRKRVQLEELRDDLELYYKLLKTAMVELINKDYAD
  FVNLSTNLVGMDKALNQLSVPLGQLREEVLSLRSSVSEGIRAVDERMSKQEDIRKKKMCVLRLIQVIRSV
  EKIEKILNSQSSKETSALEASSPLLTGQILERIATEFNQLQFHAVQSKGMPLLDKVRPRIAGITAMLQQS
  LEGLLLEGLQTSDVDIIRHCLRTYATIDKTRDAEALVGQVLVKPYIDEVIIEQFVESHPNGLQVMYNKLL
  EFVPHHCRLLREVTGGAISSEKGNTVPGYDFLVNSVWPQIVQGLEEKLPSLFNPGNPDAFHEKYTISMDF
  VRRLERQCGSQASVKRLRAHPAYHSFNKKWNLPVYFQIRFREIAGSLEAALTDVLEDAPAESPYCLLASH
  RTWSSLRRCWSDEMFLPLLVHRLWRLTLQILARYSVFVNELSLRPISNESPKEIKKPLVTGSKEPSITQG
  NTEDQGSGPSETKPVVSISRTQLVYVVADLDKLQEQLPELLEIIKPKLEMIGFKNFSSISAALEDSQSSF
  SACVPSLSSKIIQDLSDSCFGFLKSALEVPRLYRRTNKEVPTTASSYVDSALKPLFQLQSGHKDKLKQAI
  IQQWLEGTLSESTHKYYETVSDVLNSVKKMEESLKRLKQARKTTPANPVGPSGGMSDDDKIRLQLALDVE
  YLGEQIQKLGLQASDIKSFSALAELVAAAKDQATAEQP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007357
• ORF Size: 2214 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007357.3
• RefSeq Size: 2977 bp
• RefSeq ORF: 2217 bp
• Locus ID: 22796
• UniProt ID: Q14746
• Cytogenetics: 1q42.2
• Protein Families: Druggable Genome
• MW: 83.2 kDa
• Gene Summary: This gene encodes a subunit of the conserved oligomeric Golgi complex that is required for maintaining normal structure and activity of the Golgi complex. The encoded protein specifically interacts with the USO1 vesicle docking protein and may be necessary for normal Golgi ribbon formation and trafficking of Golgi enzymes. Mutations of this gene are associated with abnormal glycosylation within the Golgi apparatus. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2009]