HSPC014 (POMP) (NM_015932) Human Tagged ORF Clone
POMP (Myc-DDK-tagged)-Human proteasome maturation protein (POMP)
|Type||Human Tagged ORF Clone|
|Synonyms||C13orf12; HSPC014; PNAS-110; PRAAS2; UMP1|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC204812 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC204812 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||423 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||1477 bp|
|RefSeq ORF||426 bp|
|Gene Summary||The protein encoded by this gene is a molecular chaperone that binds 20S preproteasome components and is essential for 20S proteasome formation. The 20S proteasome is the proteolytically active component of the 26S proteasome complex. The encoded protein is degraded before the maturation of the 20S proteasome is complete. A variant in the 5' UTR of this gene has been associated with KLICK syndrome, a rare skin disorder.[provided by RefSeq, Aug 2010]|
|cDNA Clone Resources|
|RC204812L1||Lenti ORF clone of Human proteasome maturation protein (POMP), Myc-DDK-tagged||
|RC204812L2||Lenti ORF clone of Human proteasome maturation protein (POMP), mGFP tagged||
|RC204812L3||Lenti ORF clone of Human proteasome maturation protein (POMP), Myc-DDK-tagged||
|RC204812L4||Lenti ORF clone of Human proteasome maturation protein (POMP), mGFP tagged||
|RG204812||POMP (GFP-tagged) - Human proteasome maturation protein (POMP)||
|SC114530||POMP (untagged)-Human proteasome maturation protein (POMP)||