MTERF2 (NM_025198) Human Tagged ORF Clone

CAT#: RC204761

MTERFD3 (Myc-DDK-tagged)-Human MTERF domain containing 3 (MTERFD3), transcript variant 2

Product Images

Circular map for RC204761

• 

  Western blot validation of overexpression lysate (Cat# LY422353) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214531 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MTERF2
• Synonyms: MTERFD3; mTERFL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204761 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGTGGAAGCTGCTGCTGAGATCCCAGTCCTGCAGGCTGTGTTCTTTCAGAAAGATGCGATCACCTC
  CAAAATACAGACCTTTCTTAGCATGCTTCACCTATACAACTGATAAACAGTCGAGCAAAGAAAATACAAG
  AACAGTGGAAAAGCTCTATAAATGTTCAGTTGACATTAGGAAAATTCGTAGATTAAAAGGATGGGTACTT
  TTAGAGGATGAAACCTATGTTGAAGAAATTGCGAATATTTTACAAGAACTAGGTGCCGATGAGACTGCTG
  TAGCCAGTATTTTGGAACGCTGCCCGGAAGCAATTGTCTGTAGTCCAACCGCTGTTAACACCCAGAGAAA
  ACTCTGGCAGTTGGTCTGCAAAAATGAGGAAGAGTTAATCAAGTTAATAGAGCAGTTTCCAGAATCTTTC
  TTTACTATTAAAGACCAAGAGAACCAGAAGCTGAATGTTCAGTTCTTTCAAGAGTTGGGACTAAAAAATG
  TGGTCATTAGCAGACTTTTGACAGCTGCACCTAATGTTTTTCATAATCCTGTTGAGAAGAATAAGCAAAT
  GGTAAGAATTCTCCAAGAGAGTTATCTAGATGTAGGTGGCTCTGAGGCCAACATGAAAGTTTGGCTACTA
  AAATTGTTAAGCCAAAACCCATTTATTTTGTTAAATTCTCCCACAGCTATAAAGGAAACACTAGAATTTC
  TCCAGGAGCAAGGTTTCACCAGCTTTGAAATTCTCCAGCTTCTATCCAAACTCAAAGGATTTCTTTTTCA
  ACTTTGCCCAAGAAGTATACAGAATAGTATTTCCTTCTCTAAAAATGCTTTTAAATGCACAGATCATGAC
  CTGAAGCAATTAGTTTTGAAATGTCCTGCCCTTTTATATTATTCTGTTCCAGTTTTAGAAGAGAGAATGC
  AAGGATTATTGAGAGAAGGAATTTCCATAGCTCAGATAAGAGAGACGCCAATGGTTCTTGAATTAACACC
  ACAGATAGTACAGTACAGGATAAGGAAACTGAATTCCTCAGGCTACAGAATAAAGGATGGACATCTAGCA
  AATCTAAATGGATCAAAAAAAGAGTTTGAAGCTAATTTTGGCAAAATTCAGGCCAAAAAAGTAAGGCCAT
  TATTTAACCCTGTGGCACCATTAAATGTTGAAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204761 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLWKLLLRSQSCRLCSFRKMRSPPKYRPFLACFTYTTDKQSSKENTRTVEKLYKCSVDIRKIRRLKGWVL
  LEDETYVEEIANILQELGADETAVASILERCPEAIVCSPTAVNTQRKLWQLVCKNEEELIKLIEQFPESF
  FTIKDQENQKLNVQFFQELGLKNVVISRLLTAAPNVFHNPVEKNKQMVRILQESYLDVGGSEANMKVWLL
  KLLSQNPFILLNSPTAIKETLEFLQEQGFTSFEILQLLSKLKGFLFQLCPRSIQNSISFSKNAFKCTDHD
  LKQLVLKCPALLYYSVPVLEERMQGLLREGISIAQIRETPMVLELTPQIVQYRIRKLNSSGYRIKDGHLA
  NLNGSKKEFEANFGKIQAKKVRPLFNPVAPLNVEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025198
• ORF Size: 1155 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025198.4
• RefSeq Size: 1780 bp
• RefSeq ORF: 1158 bp
• Locus ID: 80298
• UniProt ID: Q49AM1
• Cytogenetics: 12q23.3
• Domains: Mterf
• MW: 44.4 kDa
• Gene Summary: Binds mitochondrial DNA and plays a role in the regulation of transcription of mitochondrial mRNA and rRNA species.[UniProtKB/Swiss-Prot Function]