ANKRD49 (NM_017704) Human Tagged ORF Clone

CAT#: RC204657

ANKRD49 (Myc-DDK-tagged)-Human ankyrin repeat domain 49 (ANKRD49)

Product Images

Circular map for RC204657

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ANKRD49 (Cat# RC204657, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANKRD49(Cat# TA507228). Positive lysates LY413612 (100ug) and LC413612 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY413612) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204657 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ANKRD49 protein (Cat# TP304657). The protein was produced from HEK293T cells transfected with ANKRD49 cDNA clone (Cat# RC204657) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ANKRD49
• Synonyms: FGIF; GBIF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204657 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAAAGAAAAAGGAAATGATGATGGAATACCAGACCAAGAGAATTCCTTGGATTTTTCTGAACACT
  TTAACCAACTTGAATTGTTGGAAACACATGGACACCTTATTCCTACTGGTACTCAAAGTCTTTGGGTAGG
  CAATTCTGATGAAGATGAGGAGCAAGATGACAAAAATGAAGAGTGGTATCGATTGCAAGAAAAAAAAATG
  GAAAAAGACCCAAGCAGATTGCTTCTTTGGGCTGCTGAAAAAAATCGGCTTACCACAGTGCGGAGACTCC
  TTTCTGAAAAGGCCACTCACGTGAACACTAGGGATGAAGATGAGTATACCCCTCTTCATCGAGCAGCCTA
  CAGTGGACACTTAGATATTGTTCAGGAGCTCATTGCACAGGGGGCCGATGTTCATGCAGTGACTGTGGAT
  GGCTGGACGCCCCTGCACAGTGCTTGTAAGTGGAATAATACCAGAGTGGCTTCTTTCTTACTGCAGCATG
  ATGCAGATATCAATGCCCAAACAAAAGGCCTCTTGACCCCCTTGCATCTTGCTGCTGGGAACAGAGACAG
  CAAGGATACCCTAGAACTCCTCCTGATGAACCGTTACGTCAAACCAGGGCTGAAAAACAACTTGGAAGAA
  ACTGCATTTGATATTGCCAGGAGGACAAGTATCTATCACTACCTCTTTGAAATTGTGGAAGGCTGTACAA
  ATTCTTCACCTCAGTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204657 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEKEKGNDDGIPDQENSLDFSEHFNQLELLETHGHLIPTGTQSLWVGNSDEDEEQDDKNEEWYRLQEKKM
  EKDPSRLLLWAAEKNRLTTVRRLLSEKATHVNTRDEDEYTPLHRAAYSGHLDIVQELIAQGADVHAVTVD
  GWTPLHSACKWNNTRVASFLLQHDADINAQTKGLLTPLHLAAGNRDSKDTLELLLMNRYVKPGLKNNLEE
  TAFDIARRTSIYHYLFEIVEGCTNSSPQS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017704
• ORF Size: 717 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017704.3
• RefSeq Size: 1924 bp
• RefSeq ORF: 720 bp
• Locus ID: 54851
• UniProt ID: Q8WVL7
• Cytogenetics: 11q21
• Domains: ANK
• MW: 27.3 kDa
• Gene Summary: Induces HBG1 expression (PubMed:16131492, PubMed:11162141). May have a role in spermatogenesis where it promotes autophagy in response to serum starvation, via the NF-kappaB pathway (By similarity).[UniProtKB/Swiss-Prot Function]