ZBTB22 (NM_005453) Human Tagged ORF Clone

CAT#: RC204562

ZBTB22 (Myc-DDK-tagged)-Human zinc finger and BTB domain containing 22 (ZBTB22), transcript variant 1

Product Images

Circular map for RC204562

• 

  Western blot validation of overexpression lysate (Cat# LY428827) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227312 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZBTB22
• Synonyms: BING1; fru; fruitless; ZBTB22A; ZNF297; ZNF297A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204562 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCATCTCCTCTGTCTCCCAGTGGGGCAGCACTTCCCCTGCCGCTGTCGCTGGCTCCGCCCCCAC
  TACCCCTGCCAGCAGCTGCAGTGGTACATGTGTCCTTCCCTGAGGTGACCAGTGCCCTCTTGGAGTCCCT
  CAATCAGCAGCGTCTGCAGGGCCAGCTCTGCGATGTATCTATCAGAGTGCAGGGCCGGGAGTTCCGGGCT
  CATCGGGCTGTCCTGGCTGCCTCCTCCCCTTACTTCCATGATCAGGTCCTACTCAAAGGCATGACCTCCA
  TCTCGCTGCCCAGTGTCATGGACCCAGGCGCCTTTGAGACTGTCCTAGCCTCCGCTTACACTGGCCGCCT
  CAGCATGGCTGCTGCTGACATTGTCAACTTCCTTACAGTGGGGTCTGTGCTCCAAATGTGGCACATTGTG
  GACAAGTGCACTGAACTACTCCGAGAAGGCCGGGCCTCAGCTACCACCACCATCACTACTGCTGCAGCCA
  CCTCTGTCACTGTCCCTGGTGCTGGGGTGCCATCCGGGAGTGGGGGCACTGTGGCCCCTGCTACCATGGG
  CTCTGCGCGCTCCCATGCCTCCAGCCGGGCCAGTGAGAATCAATCTCCCAGCAGCAGCAACTACTTCAGC
  CCCAGGGAGTCCACTGATTTCTCATCTTCCTCCCAAGAGGCATTTGCAGCTTCTGCAGTGGGCAGTGGGG
  AGCGTCGAGGAGGTGGCCCTGTATTCCCAGCCCCTGTCGTTGGCAGTGGAGGGGCCACATCTGGAAAGCT
  GCTGCTGGAGGCAGATGAGCTGTGCGATGATGGTGGGGATGGGAGGGGGGCAGTGGTTCCTGGGGCTGGG
  CTCCGGAGACCCACCTACACACCCCCTAGCATCATGCCACAGAAACACTGGGTATACGTGAAGCGAGGTG
  GTAATTGCCCAGCGCCAGCACCCCTGGTTCCCCAAGACCCAGATCTGGAGGAGGAAGAGGAGGAGGAAGA
  TCTGGTGTTGACCTGTGAGGATGATGAAGATGAAGAACTAGGGGGTAGCTCCAGGGTTCCAGTGGGGGGA
  GGGCCTGAGGCTACCCTCAGCATAAGTGATGTCCGTACCCTGAGTGAGCCCCCAGACAAGGGGGAGGAGC
  AGGTCAACTTCTGTGAGTCCTCCAATGACTTTGGCCCATATGAGGGTGGGGGTCCTGTGGCAGGTCTTGA
  TGACTCAGGGGGGCCAACTCCCTCTTCCTATGCCCCCTCCCACCCTCCTCGACCGCTCCTTCCCTTGGAC
  ATGCAGGGCAACCAGATCCTGGTCTTCCCGTCGTCGTCTTCATCCTCATCCTCACAGGCTCCTGGCCAAC
  CACCAGGGAACCAAGCAGAACACGGGGCAGTGACCGTGGGGGGCACGTCGGTGGGGAGCCTGGGTGTGCC
  GGGTAGCGTTGGTGGGGTCCCTGGAGGGACTGGCAGTGGGGACGGGAATAAGATCTTTCTGTGCCATTGT
  GGGAAGGCCTTCTCCCACAAGAGCATGCGGGACCGGCACGTGAACATGCACCTCAATCTGCGGCCGTTTG
  ACTGCCCCGTGTGCAACAAAAAGTTCAAGATGAAGCACCATCTGACTGAGCACATGAAGACGCACACAGG
  TCTCAAGCCCTACGAGTGCGGAGTCTGCGCCAAGAAGTTCATGTGGCGAGACAGCTTCATGCGCCACCGA
  GGACACTGTGAGCGCCGGCACCGCCTGGGCGGGGTCGGGGCCGTACCTGGGCCTGGGACTCCCACGGGGC
  CATCCTTGCCGTCCAAGAGAGAGTCTCCCGGAGTGGGCGGGGGCAGCGGCGACGAAGCGAGTGCGGCCAC
  GCCCCCGTCCAGCAGACGTGTCTGGTCCCCACCCAGAGTCCACAAGGTGGAGATGGGCTTCGGTGGAGGT
  GGAGGAGCAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204562 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEPSPLSPSGAALPLPLSLAPPPLPLPAAAVVHVSFPEVTSALLESLNQQRLQGQLCDVSIRVQGREFRA
  HRAVLAASSPYFHDQVLLKGMTSISLPSVMDPGAFETVLASAYTGRLSMAAADIVNFLTVGSVLQMWHIV
  DKCTELLREGRASATTTITTAAATSVTVPGAGVPSGSGGTVAPATMGSARSHASSRASENQSPSSSNYFS
  PRESTDFSSSSQEAFAASAVGSGERRGGGPVFPAPVVGSGGATSGKLLLEADELCDDGGDGRGAVVPGAG
  LRRPTYTPPSIMPQKHWVYVKRGGNCPAPAPLVPQDPDLEEEEEEEDLVLTCEDDEDEELGGSSRVPVGG
  GPEATLSISDVRTLSEPPDKGEEQVNFCESSNDFGPYEGGGPVAGLDDSGGPTPSSYAPSHPPRPLLPLD
  MQGNQILVFPSSSSSSSSQAPGQPPGNQAEHGAVTVGGTSVGSLGVPGSVGGVPGGTGSGDGNKIFLCHC
  GKAFSHKSMRDRHVNMHLNLRPFDCPVCNKKFKMKHHLTEHMKTHTGLKPYECGVCAKKFMWRDSFMRHR
  GHCERRHRLGGVGAVPGPGTPTGPSLPSKRESPGVGGGSGDEASAATPPSSRRVWSPPRVHKVEMGFGGG
  GGAN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005453
• ORF Size: 1902 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005453.3, NP_005444.3
• RefSeq Size: 2681 bp
• RefSeq ORF: 1905 bp
• Locus ID: 9278
• UniProt ID: O15209
• Cytogenetics: 6p21.32
• Domains: BTB, zf-C2H2
• Protein Families: Transcription Factors
• MW: 65.6 kDa
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]