RNF113A (NM_006978) Human Tagged ORF Clone

CAT#: RC204528

RNF113A (Myc-DDK-tagged)-Human ring finger protein 113A (RNF113A)

Product Images

Circular map for RC204528

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  Western blot validation of overexpression lysate (Cat# LY416295) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204528 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RNF113A protein (Cat# TP304528). The protein was produced from HEK293T cells transfected with RNF113A cDNA clone (Cat# RC204528) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RNF113A
• Synonyms: Cwc24; RNF113; TTD5; ZNF183
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204528 representing NM_006978
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGCAGCTTTCTCCAGGAAAGGCGGTGGATCAGGTGTGCACCTTCCTTTTCAAAAAGCCTGGGC
  GGAAAGGGGCTGCTGGACGCAGAAAGCGCCCGGCCTGCGACCCAGAGCCCGGAGAAAGCGGCAGCAGTAG
  CGACGAAGGCTGCACTGTGGTTCGACCGGAAAAGAAGCGGGTGACCCACAATCCAATGATACAGAAGACC
  CGTGACAGTGGTAAACAGAAGGCGGCTTACGGCGACTTGAGCAGCGAAGAGGAAGAGGAAAATGAGCCCG
  AGAGTCTCGGCGTGGTTTATAAATCCACCCGTTCGGCGAAACCCGTGGGACCAGAGGATATGGGAGCGAC
  AGCTGTCTATGAGCTGGACACAGAGAAAGAGCGCGATGCACAAGCCATCTTTGAGCGCAGCCAGAAGATC
  CAGGAGGAGCTGAGGGGCAAGGAGGATGACAAGATCTATCGGGGAATCAACAATTATCAGAAATACATGA
  AGCCCAAGGATACGTCTATGGGCAATGCCTCTTCCGGGATGGTGAGGAAGGGCCCCATCCGAGCGCCCGA
  GCATCTACGTGCCACCGTGCGCTGGGATTACCAGCCCGACATCTGTAAGGACTACAAAGAGACTGGCTTC
  TGCGGCTTCGGAGACAGCTGCAAATTCCTCCATGACCGTTCAGATTACAAGCATGGGTGGCAGATCGAAC
  GTGAGCTTGATGAGGGTCGCTATGGTGTCTATGAGGATGAAAACTATGAAGTGGGAAGCGATGATGAGGA
  AATACCATTCAAGTGTTTCATCTGTCGCCAGAGCTTCCAAAACCCAGTTGTCACCAAGTGCAGGCATTAT
  TTCTGCGAGAGCTGTGCACTGCAGCATTTCCGCACCACCCCGCGCTGCTATGTCTGTGACCAGCAGACCA
  ATGGCGTCTTCAATCCAGCGAAAGAATTGATTGCTAAACTAGAGAAGCATCGAGCTACAGGAGAGGGTGG
  TGCTTCCGACTTGCCAGAAGACCCCGATGAGGATGCAATTCCCATTACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204528 representing NM_006978
  Red=Cloning site Green=Tags(s)
  
  MAEQLSPGKAVDQVCTFLFKKPGRKGAAGRRKRPACDPEPGESGSSSDEGCTVVRPEKKRVTHNPMIQKT
  RDSGKQKAAYGDLSSEEEEENEPESLGVVYKSTRSAKPVGPEDMGATAVYELDTEKERDAQAIFERSQKI
  QEELRGKEDDKIYRGINNYQKYMKPKDTSMGNASSGMVRKGPIRAPEHLRATVRWDYQPDICKDYKETGF
  CGFGDSCKFLHDRSDYKHGWQIERELDEGRYGVYEDENYEVGSDDEEIPFKCFICRQSFQNPVVTKCRHY
  FCESCALQHFRTTPRCYVCDQQTNGVFNPAKELIAKLEKHRATGEGGASDLPEDPDEDAIPIT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006978
• ORF Size: 1029 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006978.3
• RefSeq Size: 1349 bp
• RefSeq ORF: 1032 bp
• Locus ID: 7737
• UniProt ID: O15541
• Cytogenetics: Xq24
• Domains: zf-CCCH, RING
• Protein Families: Druggable Genome, Protease
• MW: 38.6 kDa
• Gene Summary: This intronless gene encodes a protein which contains a C3H1-type zinc finger domain and a C3HC4 Ring-type (Really Interesting New Gene-type) zinc finger domain. The Ring-type zinc finger domain is identified in various tumor suppressors, DNA repair genes and cytokine receptor-associated molecules, and is probably involved in mediating protein-protein interactions. [provided by RefSeq, May 2010]