PP2A-beta (PPP2CB) (NM_001009552) Human Tagged ORF Clone

CAT#: RC204491

PPP2CB (Myc-DDK-tagged)-Human protein phosphatase 2, catalytic subunit, beta isozyme (PPP2CB)

Product Images

Circular map for RC204491

• 

  Western blot validation of overexpression lysate (Cat# LY418183) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219083 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PP2A-beta
• Synonyms: PP2Abeta; PP2CB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204491 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGACAAGGCGTTCACCAAGGAGCTGGACCAGTGGGTCGAGCAGCTGAACGAGTGTAAGCAGCTGA
  ACGAGAACCAAGTGCGGACGCTGTGCGAGAAGGCAAAGGAAATTTTAACAAAAGAATCAAATGTGCAAGA
  GGTTCGTTGCCCTGTTACTGTCTGTGGAGATGTGCATGGTCAATTTCATGATCTTATGGAACTCTTTAGA
  ATTGGTGGAAAATCACCGGATACAAACTACTTATTCATGGGTGACTATGTAGACAGAGGATATTATTCAG
  TGGAGACTGTGACTCTTCTTGTAGCATTAAAGGTGCGTTATCCAGAACGCATTACAATATTGAGAGGAAA
  TCACGAAAGCCGACAAATTACCCAAGTATATGGCTTTTATGATGAATGTCTGCGAAAGTATGGGAATGCC
  AACGTTTGGAAATATTTTACAGATCTCTTTGATTATCTTCCACTTACAGCTTTAGTAGATGGACAGATAT
  TCTGCCTCCATGGTGGCCTCTCTCCATCCATAGACACACTGGATCATATAAGAGCCCTGGATCGTTTACA
  GGAAGTTCCACATGAGGGCCCAATGTGTGATCTGTTATGGTCAGATCCAGATGATCGTGGTGGATGGGGT
  ATTTCACCACGTGGTGCTGGCTACACATTTGGACAAGACATTTCTGAAACCTTTAACCATGCCAATGGTC
  TCACACTGGTTTCTCGTGCCCACCAGCTTGTAATGGAGGGATACAATTGGTGTCATGATCGGAATGTGGT
  TACCATTTTCAGTGCACCCAATTACTGTTATCGTTGTGGGAACCAGGCTGCTATCATGGAATTAGATGAC
  ACTTTAAAATATTCCTTCCTTCAATTTGACCCAGCGCCTCGTCGTGGTGAGCCTCATGTTACACGGCGCA
  CCCCAGACTACTTCCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204491 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDDKAFTKELDQWVEQLNECKQLNENQVRTLCEKAKEILTKESNVQEVRCPVTVCGDVHGQFHDLMELFR
  IGGKSPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYPERITILRGNHESRQITQVYGFYDECLRKYGNA
  NVWKYFTDLFDYLPLTALVDGQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLWSDPDDRGGWG
  ISPRGAGYTFGQDISETFNHANGLTLVSRAHQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAAIMELDD
  TLKYSFLQFDPAPRRGEPHVTRRTPDYFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001009552
• ORF Size: 927 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009552.2
• RefSeq Size: 1987 bp
• RefSeq ORF: 930 bp
• Locus ID: 5516
• UniProt ID: P62714
• Cytogenetics: 8p12
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway
• MW: 35.6 kDa
• Gene Summary: This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes a beta isoform of the catalytic subunit. [provided by RefSeq, Mar 2010]