XPC (NM_004628) Human Tagged ORF Clone

CAT#: RC204483

XPC (Myc-DDK-tagged)-Human xeroderma pigmentosum, complementation group C (XPC), transcript variant 1

Product Images

Circular map for RC204483

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY XPC (Cat# RC204483, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XPC rabbit polyclonal antibody (Cat# TA890156).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: XPC
• Synonyms: p125; RAD4; XP3; XPCC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204483 representing NM_004628
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCGGAAACGCGCGGCCGGCGGGGAGCCGCGGGGACGCGAACTGCGCAGCCAGAAATCCAAGGCCA
  AGAGCAAGGCCCGGCGTGAGGAGGAGGAGGAGGATGCCTTTGAAGATGAGAAACCCCCAAAGAAGAGCCT
  TCTCTCCAAAGTTTCACAAGGAAAGAGGAAAAGAGGCTGCAGTCATCCTGGGGGTTCAGCAGATGGTCCA
  GCAAAAAAGAAAGTGGCCAAGGTGACTGTTAAATCTGAAAACCTCAAGGTTATAAAGGATGAAGCCCTCA
  GCGATGGGGATGACCTCAGGGACTTTCCAAGTGACCTCAAGAAGGCACACCATCTGAAGAGAGGGGCTAC
  CATGAATGAAGACAGCAATGAAGAAGAGGAAGAAAGTGAAAATGATTGGGAAGAGGTTGAAGAACTTAGT
  GAGCCTGTGCTGGGTGACGTGAGAGAAAGTACAGCCTTCTCTCGATCTCTTCTGCCTGTGAAGCCAGTGG
  AGATAGAGATTGAAACGCCAGAGCAGGCGAAGACAAGAGAAAGAAGTGAAAAGATAAAACTGGAGTTTGA
  GACATATCTTCGGAGGGCGATGAAACGTTTCAATAAAGGGGTCCATGAGGACACACACAAGGTTCACCTT
  CTCTGCCTGCTAGCAAATGGCTTCTATCGAAATAACATCTGCAGCCAGCCAGATCTGCATGCTATTGGCC
  TGTCCATCATCCCAGCCCGCTTTACCAGAGTGCTGCCTCGAGATGTGGACACCTACTACCTCTCAAACCT
  GGTGAAGTGGTTCATTGGAACATTTACAGTTAATGCAGAACTTTCAGCCAGTGAACAAGATAACCTGCAG
  ACTACATTGGAAAGGAGATTTGCTATTTACTCTGCTCGAGATGATGAGGAATTGGTCCATATATTCTTAC
  TGATTCTCCGGGCTCTGCAGCTCTTGACCCGGCTGGTATTGTCTCTACAGCCAATTCCTCTGAAGTCAGC
  AACAGCAAAGGGAAAGAAACCTTCCAAGGAAAGATTGACTGCGGATCCAGGAGGCTCCTCAGAAACTTCC
  AGCCAAGTTCTAGAAAACCACACCAAACCAAAGACCAGCAAAGGAACCAAACAAGAGGAAACCTTTGCTA
  AGGGCACCTGCAGGCCAAGTGCCAAAGGGAAGAGGAACAAGGGAGGCAGAAAGAAACGGAGCAAGCCCTC
  CTCCAGCGAGGAAGATGAGGGCCCAGGAGACAAGCAGGAGAAGGCAACCCAGCGACGTCCGCATGGCCGG
  GAGCGGCGGGTGGCCTCCAGGGTGTCTTATAAAGAGGAGAGTGGGAGTGATGAGGCTGGCAGCGGCTCTG
  ATTTTGAGCTCTCCAGTGGAGAAGCCTCTGATCCCTCTGATGAGGATTCCGAACCTGGCCCTCCAAAGCA
  GAGGAAAGCCCCCGCTCCTCAGAGGACAAAGGCTGGGTCCAAGAGTGCCTCCAGGACCCATCGTGGGAGC
  CATCGTAAGGACCCAAGCTTGCCAGCGGCATCCTCAAGCTCTTCAAGCAGTAAAAGAGGCAAGAAAATGT
  GCAGCGATGGTGAGAAGGCAGAAAAAAGAAGCATAGCTGGTATAGACCAGTGGCTAGAGGTGTTCTGTGA
  GCAGGAGGAAAAGTGGGTATGTGTAGACTGTGTGCACGGTGTGGTGGGCCAGCCTCTGACCTGTTACAAG
  TACGCCACCAAGCCCATGACCTATGTGGTGGGCATTGACAGTGACGGCTGGGTCCGAGATGTCACACAGA
  GGTACGACCCAGTCTGGATGACAGTGACCCGCAAGTGCCGGGTTGATGCTGAGTGGTGGGCCGAGACCTT
  GAGACCATACCAGAGCCCATTTATGGACAGGGAGAAGAAAGAAGACTTGGAGTTTCAGGCAAAACACATG
  GACCAGCCTTTGCCCACTGCCATTGGCTTATATAAGAACCACCCTCTGTATGCCCTGAAGCGGCATCTCC
  TGAAATATGAGGCCATCTATCCCGAGACAGCTGCCATCCTTGGGTATTGTCGTGGAGAAGCGGTCTACTC
  CAGGGATTGTGTGCACACTCTGCATTCCAGAGACACGTGGCTGAAGAAAGCAAGAGTGGTGAGGCTTGGA
  GAAGTACCCTACAAGATGGTGAAAGGCTTTTCTAACCGTGCTCGGAAAGCCCGACTTGCTGAGCCCCAGC
  TGCGGGAAGAAAATGACCTGGGCCTGTTTGGCTACTGGCAGACAGAGGAGTATCAGCCCCCAGTGGCCGT
  GGACGGGAAGGTGCCCCGGAACGAGTTTGGGAATGTGTACCTCTTCCTGCCCAGCATGATGCCTATTGGC
  TGTGTCCAGCTGAACCTGCCCAATCTACACCGCGTGGCCCGCAAGCTGGACATCGACTGTGTCCAGGCCA
  TCACTGGCTTTGATTTCCATGGCGGCTACTCCCATCCCGTGACTGATGGATACATCGTCTGCGAGGAATT
  CAAAGACGTGCTCCTGACTGCCTGGGAAAATGAGCAGGCAGTCATTGAAAGGAAGGAGAAGGAGAAAAAG
  GAGAAGCGGGCTCTAGGGAACTGGAAGTTGCTGGCCAAAGGTCTGCTCATCAGGGAGAGGCTGAAGCGTC
  GCTACGGGCCCAAGAGTGAGGCAGCAGCTCCCCACACAGATGCAGGAGGTGGACTCTCTTCTGATGAAGA
  GGAGGGGACCAGCTCTCAAGCAGAAGCGGCCAGGATACTGGCTGCCTCCTGGCCTCAAAACCGAGAAGAT
  GAAGAAAAGCAGAAGCTGAAGGGTGGGCCCAAGAAGACCAAAAGGGAAAAGAAAGCAGCAGCTTCCCACC
  TGTTCCCATTTGAGAAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204483 representing NM_004628
  Red=Cloning site Green=Tags(s)
  
  MARKRAAGGEPRGRELRSQKSKAKSKARREEEEEDAFEDEKPPKKSLLSKVSQGKRKRGCSHPGGSADGP
  AKKKVAKVTVKSENLKVIKDEALSDGDDLRDFPSDLKKAHHLKRGATMNEDSNEEEEESENDWEEVEELS
  EPVLGDVRESTAFSRSLLPVKPVEIEIETPEQAKTRERSEKIKLEFETYLRRAMKRFNKGVHEDTHKVHL
  LCLLANGFYRNNICSQPDLHAIGLSIIPARFTRVLPRDVDTYYLSNLVKWFIGTFTVNAELSASEQDNLQ
  TTLERRFAIYSARDDEELVHIFLLILRALQLLTRLVLSLQPIPLKSATAKGKKPSKERLTADPGGSSETS
  SQVLENHTKPKTSKGTKQEETFAKGTCRPSAKGKRNKGGRKKRSKPSSSEEDEGPGDKQEKATQRRPHGR
  ERRVASRVSYKEESGSDEAGSGSDFELSSGEASDPSDEDSEPGPPKQRKAPAPQRTKAGSKSASRTHRGS
  HRKDPSLPAASSSSSSSKRGKKMCSDGEKAEKRSIAGIDQWLEVFCEQEEKWVCVDCVHGVVGQPLTCYK
  YATKPMTYVVGIDSDGWVRDVTQRYDPVWMTVTRKCRVDAEWWAETLRPYQSPFMDREKKEDLEFQAKHM
  DQPLPTAIGLYKNHPLYALKRHLLKYEAIYPETAAILGYCRGEAVYSRDCVHTLHSRDTWLKKARVVRLG
  EVPYKMVKGFSNRARKARLAEPQLREENDLGLFGYWQTEEYQPPVAVDGKVPRNEFGNVYLFLPSMMPIG
  CVQLNLPNLHRVARKLDIDCVQAITGFDFHGGYSHPVTDGYIVCEEFKDVLLTAWENEQAVIERKEKEKK
  EKRALGNWKLLAKGLLIRERLKRRYGPKSEAAAPHTDAGGGLSSDEEEGTSSQAEAARILAASWPQNRED
  EEKQKLKGGPKKTKREKKAAASHLFPFEKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004628
• ORF Size: 2820 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004628.2
• RefSeq Size: 3714 bp
• RefSeq ORF: 2823 bp
• Locus ID: 7508
• UniProt ID: Q01831
• Cytogenetics: 3p25.1
• Domains: Rad4
• Protein Families: Druggable Genome
• Protein Pathways: Nucleotide excision repair
• MW: 105.8 kDa
• Gene Summary: The protein encoded by this gene is a key component of the XPC complex, which plays an important role in the early steps of global genome nucleotide excision repair (NER). The encoded protein is important for damage sensing and DNA binding, and shows a preference for single-stranded DNA. Mutations in this gene or some other NER components can result in Xeroderma pigmentosum, a rare autosomal recessive disorder characterized by increased sensitivity to sunlight with the development of carcinomas at an early age. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2017]