Syntrophin alpha 1 (SNTA1) (NM_003098) Human Tagged ORF Clone

CAT#: RC204480

SNTA1 (Myc-DDK-tagged)-Human syntrophin, alpha 1 (dystrophin-associated protein A1, 59kDa, acidic component) (SNTA1)

Product Images

Circular map for RC204480

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SNTA1 (Cat# RC204480, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNTA1(Cat# TA502042). Positive lysates LY401080 (100ug) and LC401080 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY401080) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204480 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SNTA1 protein (Cat# TP304480). The protein was produced from HEK293T cells transfected with SNTA1 cDNA clone (Cat# RC204480) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Syntrophin alpha 1
• Synonyms: dJ1187J4.5; LQT12; SNT1; TACIP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204480 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCGGCAGGCGCGCCCCGCGCACCGGGCTGCTGGAGCTGCGCGCCGGGGCGGGCTCGGGGGCCG
  GCGGCGAGCGATGGCAGCGGGTGCTGCTGAGTCTGGCGGAGGACGTGCTGACCGTGAGCCCCGCCGACGG
  CGACCCTGGTCCCGAGCCCGGCGCTCCGCGGGAGCAGGAGCCCGCGCAGCTCAACGGCGCCGCGGAGCCG
  GGCGCCGGGCCCCCGCAGCTGCCAGAGGCGCTACTGCTCCAGCGGCGCCGCGTGACGGTGCGCAAGGCCG
  ACGCCGGTGGGCTGGGCATCAGCATCAAAGGCGGCCGGGAGAACAAGATGCCTATTCTCATTTCCAAGAT
  CTTCAAGGGATTGGCAGCTGACCAGACAGAGGCCCTTTTTGTGGGGGATGCCATCCTGTCTGTGAATGGG
  GAAGACTTGTCCTCTGCTACCCATGATGAGGCGGTGCAGGTCCTCAAGAAGACAGGCAAGGAGGTGGTGC
  TGGAGGTCAAGTATATGAAGGACGTCTCACCGTATTTCAAGAACTCTACTGGTGGGACCTCGGTCGGCTG
  GGACTCACCTCCTGCCTCACCCCTTCAGCGGCAGCCTTCCTCCCCTGGCCCCACACCCCGGAACTTCAGC
  GAGGCCAAACACATGTCCTTGAAGATGGCATATGTCTCGAAGAGGTGCACCCCCAATGACCCGGAGCCCA
  GGTATCTGGAGATCTGCTCGGCAGATGGTCAAGACACCCTCTTCCTGAGGGCCAAGGATGAGGCTAGTGC
  GAGGTCGTGGGCGACTGCCATCCAAGCCCAGGTCAATACTCTGACGCCGCGGGTCAAGGATGAGCTGCAG
  GCACTGTTGGCAGCCACCAGCACAGCTGGGAGCCAGGACATCAAGCAGATTGGCTGGCTAACTGAGCAGC
  TGCCCAGTGGGGGCACAGCCCCCACCCTGGCCCTGCTAACTGAAAAGGAACTGCTCCTCTACTTGTCTCT
  CCCCGAGACCCGCGAGGCCCTGAGCCGGCCAGCCCGTACTGCCCCACTCATCGCCACCAGACTGGTGCAC
  TCAGGCCCCTCCAAGGGCTCAGTGCCCTACGATGCAGAGCTCTCTTTTGCCCTGCGCACGGGCACGCGTC
  ACGGTGTGGACACTCACCTGTTCAGCGTGGAGTCACCGCAGGAGCTGGCTGCCTGGACCCGCCAGCTTGT
  GGATGGCTGTCACCGGGCCGCCGAGGGTGTGCAGGAGGTGTCTACAGCCTGCACGTGGAATGGGCGTCCC
  TGCAGCCTGTCTGTGCACATCGACAAGGGCTTCACACTGTGGGCGGCTGAGCCAGGTGCAGCCCGAGCTG
  TGCTCCTGCGACAGCCCTTCGAGAAGCTGCAGATGTCTTCAGATGACGGTGCCAGTCTCCTTTTCCTGGA
  TTTTGGAGGTGCTGAAGGCGAGATCCAGCTGGACCTGCACTCGTGTCCCAAAACCATAGTCTTCATCATC
  CACTCCTTCCTGTCGGCCAAAGTCACCCGCCTCGGGCTGTTGGCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204480 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASGRRAPRTGLLELRAGAGSGAGGERWQRVLLSLAEDVLTVSPADGDPGPEPGAPREQEPAQLNGAAEP
  GAGPPQLPEALLLQRRRVTVRKADAGGLGISIKGGRENKMPILISKIFKGLAADQTEALFVGDAILSVNG
  EDLSSATHDEAVQVLKKTGKEVVLEVKYMKDVSPYFKNSTGGTSVGWDSPPASPLQRQPSSPGPTPRNFS
  EAKHMSLKMAYVSKRCTPNDPEPRYLEICSADGQDTLFLRAKDEASARSWATAIQAQVNTLTPRVKDELQ
  ALLAATSTAGSQDIKQIGWLTEQLPSGGTAPTLALLTEKELLLYLSLPETREALSRPARTAPLIATRLVH
  SGPSKGSVPYDAELSFALRTGTRHGVDTHLFSVESPQELAAWTRQLVDGCHRAAEGVQEVSTACTWNGRP
  CSLSVHIDKGFTLWAAEPGAARAVLLRQPFEKLQMSSDDGASLLFLDFGGAEGEIQLDLHSCPKTIVFII
  HSFLSAKVTRLGLLA
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003098
• ORF Size: 1515 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003098.3
• RefSeq Size: 2345 bp
• RefSeq ORF: 1518 bp
• Locus ID: 6640
• UniProt ID: Q13424
• Cytogenetics: 20q11.21
• Domains: PDZ, PH
• MW: 53.9 kDa
• Gene Summary: Syntrophins are cytoplasmic peripheral membrane scaffold proteins that are components of the dystrophin-associated protein complex. This gene is a member of the syntrophin gene family and encodes the most common syntrophin isoform found in cardiac tissues. The N-terminal PDZ domain of this syntrophin protein interacts with the C-terminus of the pore-forming alpha subunit (SCN5A) of the cardiac sodium channel Nav1.5. This protein also associates cardiac sodium channels with the nitric oxide synthase-PMCA4b (plasma membrane Ca-ATPase subtype 4b) complex in cardiomyocytes. This gene is a susceptibility locus for Long-QT syndrome (LQT) - an inherited disorder associated with sudden cardiac death from arrhythmia - and sudden infant death syndrome (SIDS). This protein also associates with dystrophin and dystrophin-related proteins at the neuromuscular junction and alters intracellular calcium ion levels in muscle tissue. [provided by RefSeq, Jan 2013]