SEC62 (NM_003262) Human Tagged ORF Clone

CAT#: RC204452

SEC62 (Myc-DDK-tagged)-Human SEC62 homolog (S. cerevisiae) (SEC62)

Product Images

Circular map for RC204452

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  Western blot validation of overexpression lysate (Cat# LY418802) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204452 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SEC62 protein (Cat# TP304452). The protein was produced from HEK293T cells transfected with SEC62 cDNA clone (Cat# RC204452) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SEC62
• Synonyms: Dtrp1; HTP1; TLOC1; TP-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204452 representing NM_003262
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAACGCAGGAGACACAAGAAGCGGATCCAGGAAGTTGGTGAACCATCTAAAGAAGAGAAGGCTG
  TGGCCAAGTATCTTCGATTCAACTGTCCAACAAAGTCCACCAATATGATGGGTCACCGGGTTGATTATTT
  TATTGCTTCAAAAGCAGTGGACTGTCTTTTGGATTCAAAGTGGGCAAAGGCCAAGAAAGGAGAGGAAGCT
  TTATTTACAACCAGGGAGTCTGTGGTTGACTACTGCAACAGGCTTTTAAAGAAGCAGTTTTTTCACCGAG
  CCCTAAAAGTAATGAAAATGAAATATGATAAAGACATAAAGAAAGAAAAAGATAAAGGAAAAGCTGAAAG
  TGGAAAAGAAGAAGATAAAAAGAGCAAGAAAGAAAATATAAAGGATGAGAAGACAAAAAAAGAAAAAGAG
  AAAAAAAAAGATGGTGAAAAGGAAGAATCCAAAAAGGAGGAAACTCCAGGAACTCCTAAAAAGAAGGAAA
  CTAAGAAAAAATTCAAACTTGAGCCACATGATGATCAGGTTTTTCTGGATGGAAATGAGGTGTATGTATG
  GATCTATGACCCAGTTCACTTTAAAACATTTGTCATGGGATTAATTCTTGTGATTGCAGTAATAGCGGCC
  ACCCTCTTCCCCCTTTGGCCAGCAGAAATGAGAGTAGGTGTTTATTACCTCAGTGTGGGTGCAGGCTGTT
  TTGTAGCCAGTATTCTTCTCCTTGCTGTTGCTCGATGCATTCTATTTCTCATCATTTGGCTCATAACTGG
  AGGAAGGCACCACTTTTGGTTCTTGCCAAATCTGACTGCTGATGTGGGCTTCATTGACTCCTTCAGGCCT
  CTGTACACACATGAATACAAAGGACCAAAAGCAGACTTAAAGAAAGATGAGAAGTCTGAAACCAAAAAGC
  AACAGAAGTCCGACAGTGAGGAAAAGTCAGACAGTGAGAAAAAGGAAGATGAGGAGGGGAAAGTAGGACC
  AGGAAATCATGGAACAGAAGGCTCGGGGGGAGAACGGCATTCAGACACGGACAGTGACAGGAGGGAAGAT
  GATCGATCCCAGCACAGTAGTGGAAATGGAAATGATTTTGAAATGATAACAAAAGAGGAACTGGAACAGC
  AAACAGATGGGGATTGTGAAGAGGATGAGGAAGAGGAAAATGATGGAGAAACACCTAAATCTTCACATGA
  AAAATCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204452 representing NM_003262
  Red=Cloning site Green=Tags(s)
  
  MAERRRHKKRIQEVGEPSKEEKAVAKYLRFNCPTKSTNMMGHRVDYFIASKAVDCLLDSKWAKAKKGEEA
  LFTTRESVVDYCNRLLKKQFFHRALKVMKMKYDKDIKKEKDKGKAESGKEEDKKSKKENIKDEKTKKEKE
  KKKDGEKEESKKEETPGTPKKKETKKKFKLEPHDDQVFLDGNEVYVWIYDPVHFKTFVMGLILVIAVIAA
  TLFPLWPAEMRVGVYYLSVGAGCFVASILLLAVARCILFLIIWLITGGRHHFWFLPNLTADVGFIDSFRP
  LYTHEYKGPKADLKKDEKSETKKQQKSDSEEKSDSEKKEDEEGKVGPGNHGTEGSGGERHSDTDSDRRED
  DRSQHSSGNGNDFEMITKEELEQQTDGDCEEDEEEENDGETPKSSHEKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003262
• ORF Size: 1197 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003262.4
• RefSeq Size: 6541 bp
• RefSeq ORF: 1200 bp
• Locus ID: 7095
• UniProt ID: Q99442
• Cytogenetics: 3q26.2
• Domains: Sec62
• Protein Families: Druggable Genome, Transmembrane
• MW: 45.7 kDa
• Gene Summary: The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. The protein encoded by this gene and SEC63 protein are found to be associated with ribosome-free SEC61 complex. It is speculated that Sec61-Sec62-Sec63 may perform post-translational protein translocation into the ER. The Sec61-Sec62-Sec63 complex might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. The encoded protein is an integral membrane protein located in the rough ER. [provided by RefSeq, Jul 2008]