Map17 (PDZK1IP1) (NM_005764) Human Tagged ORF Clone

CAT#: RC204437

PDZK1IP1 (Myc-DDK-tagged)-Human PDZK1 interacting protein 1 (PDZK1IP1)

Product Images

Circular map for RC204437

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  Western blot validation of overexpression lysate (Cat# LY417088) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204437 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PDZK1IP1 protein (Cat# TP304437). The protein was produced from HEK293T cells transfected with PDZK1IP1 cDNA clone (Cat# RC204437) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Map17
• Synonyms: DD96; MAP17; SPAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204437 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCCCTCAGCCTCCTCATTCTGGGCCTGCTCATGGCAGTGCCACCTGCCAGCTGTCAGCAAGGCC
  TGGGGAACCTTCAGCCCTGGATGCAGGGCCTTATCGCGGTGGCCGTGTTCCTGGTCCTCGTTGCAATCGC
  CTTTGCAGTCAAACACTTCTGGTGCCAGGAGGAGCCGGAGCCTGCACACATGATCCTGACCGTCGGAAAC
  AAGGCAGATGGAGTCCTGGTGGGAACAGATGGAAGGTACTCTTCGATGGCGGCCAGTTTCAGGTCCAGTG
  AGCATGAGAATGCCTATGAGAATGTGCCCGAGGAGGAAGGCAAGGTCCGCAGCACCCCGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204437 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSALSLLILGLLMAVPPASCQQGLGNLQPWMQGLIAVAVFLVLVAIAFAVKHFWCQEEPEPAHMILTVGN
  KADGVLVGTDGRYSSMAASFRSSEHENAYENVPEEEGKVRSTPM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005764
• ORF Size: 342 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005764.4
• RefSeq Size: 894 bp
• RefSeq ORF: 345 bp
• Locus ID: 10158
• UniProt ID: Q13113
• Cytogenetics: 1p33
• Protein Families: Transmembrane
• MW: 12.3 kDa
• Gene Summary: May play an important role in tumor biology.[UniProtKB/Swiss-Prot Function]