UBE2Q2 (NM_173469) Human Tagged ORF Clone

CAT#: RC204433

UBE2Q2 (Myc-DDK-tagged)-Human ubiquitin-conjugating enzyme E2Q family member 2 (UBE2Q2), transcript variant 1

Product Images

Circular map for RC204433

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  Western blot validation of overexpression lysate (Cat# LY406622) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204433 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified UBE2Q2 protein (Cat# TP304433). The protein was produced from HEK293T cells transfected with UBE2Q2 cDNA clone (Cat# RC204433) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UBE2Q2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204433 representing NM_173469
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGTGTCAGGGCTCAAGGCCGAGCTGAAGTTCCTGGCGTCCATCTTCGACAAGAACCACGAGCGAT
  TCCGCATCGTCAGTTGGAAGCTGGACGAGCTGCACTGCCAGTTCCTGGTGCCGCAGCAGGGCAGCCCGCA
  CTCGCTGCCGCCGCCACTCACGCTCCACTGCAACATCACGGAATCCTATCCATCTTCTTCACCGATATGG
  TTTGTGGATTCTGAAGACCCAAATCTGACATCAGTTCTGGAACGTCTAGAAGATACTAAGAACAACAATT
  TGCTTCGTCAGCAATTGAAGTGGTTGATATGTGAACTCTGCAGTTTATATAACCTTCCTAAGCACCTGGA
  TGTTGAGATGCTAGATCAACCACTACCCACGGGTCAGAATGGGACAACAGAAGAAGTGACTTCAGAAGAA
  GAGGAAGAAGAAGAAGAGATGGCTGAAGATATAGAAGACTTAGATCACTATGAGATGAAGGAAGAAGAGC
  CTATTAGTGGGAAAAAGTCAGAGGATGAAGGAATTGAAAAAGAAAATTTGGCAATATTAGAGAAAATTAG
  GAAGACTCAAAGGCAAGACCATTTAAATGGTGCAGTGTCTGGGTCAGTGCAAGCTTCAGATAGACTTATG
  AAAGAGCTCAGGGACATATACAGATCACAGAGTTATAAAACAGGGATTTATTCAGTGGAACTCATAAATG
  ACAGTTTATATGACTGGCATGTTAAACTGCAGAAGGTTGACCCTGATAGTCCTTTGCACAGTGATCTTCA
  GATCTTAAAAGAAAAAGAAGGCATAGAATATATTTTGCTTAACTTCTCTTTTAAGGATAACTTTCCATTT
  GATCCTCCATTTGTTCGAGTGGTGTTACCTGTTCTCTCAGGAGGGTATGTATTGGGTGGAGGAGCATTAT
  GTATGGAACTTCTCACAAAACAGGGCTGGAGCAGTGCCTACTCAATAGAATCGGTCATCATGCAAATAAA
  TGCCACCTTAGTCAAAGGCAAAGCCAGAGTGCAGTTTGGAGCAAATAAGAATCAATATAATCTAGCAAGA
  GCCCAACAATCCTATAATTCCATTGTACAGATACATGAGAAAAATGGCTGGTACACCCCTCCAAAGGAAG
  ATGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204433 representing NM_173469
  Red=Cloning site Green=Tags(s)
  
  MSVSGLKAELKFLASIFDKNHERFRIVSWKLDELHCQFLVPQQGSPHSLPPPLTLHCNITESYPSSSPIW
  FVDSEDPNLTSVLERLEDTKNNNLLRQQLKWLICELCSLYNLPKHLDVEMLDQPLPTGQNGTTEEVTSEE
  EEEEEEMAEDIEDLDHYEMKEEEPISGKKSEDEGIEKENLAILEKIRKTQRQDHLNGAVSGSVQASDRLM
  KELRDIYRSQSYKTGIYSVELINDSLYDWHVKLQKVDPDSPLHSDLQILKEKEGIEYILLNFSFKDNFPF
  DPPFVRVVLPVLSGGYVLGGGALCMELLTKQGWSSAYSIESVIMQINATLVKGKARVQFGANKNQYNLAR
  AQQSYNSIVQIHEKNGWYTPPKEDG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173469
• ORF Size: 1125 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173469.4
• RefSeq Size: 2939 bp
• RefSeq ORF: 1128 bp
• Locus ID: 92912
• UniProt ID: Q8WVN8
• Cytogenetics: 15q24.2
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 42.6 kDa
• Gene Summary: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination.[UniProtKB/Swiss-Prot Function]