TBC1D5 (NM_014744) Human Tagged ORF Clone

CAT#: RC204401

TBC1D5 (Myc-DDK-tagged)-Human TBC1 domain family, member 5 (TBC1D5), transcript variant 2

Product Images

Circular map for RC204401

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  Western blot validation of overexpression lysate (Cat# LY427408) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226171 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TBC1D5 protein (Cat# TP304401). The protein was produced from HEK293T cells transfected with TBC1D5 cDNA clone (Cat# RC204401) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TBC1D5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204401 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATCATTCCTTATCTGAAACTAGACATCCTCTGCAGCCAGAAGAACAAGAAGTAGGCATTGACCCCT
  TGTCCAGTTACTCTAACAAGTCTGGAGGAGATTCAAATAAAAATGGAAGAAGAACAAGTTCTACTTTAGA
  CTCTGAAGGGACTTTTAATTCCTATAGGAAAGAATGGGAAGAACTATTTGTAAACAACAATTACTTGGCA
  ACAATAAGGCAGAAGGGGATTAATGGGCAGCTGAGAAGCAGCAGGTTCCGCAGCATTTGCTGGAAGCTAT
  TTCTTTGTGTTCTTCCTCAAGACAAAAGTCAATGGATAAGTAGAATTGAAGAATTAAGAGCATGGTATAG
  CAACATTAAAGAAATACATATTACCAACCCGAGGAAGGTTGTTGGCCAACAAGATTTGATGATCAATAAT
  CCTCTTTCACAGGATGAAGGGAGTCTTTGGAACAAATTCTTCCAAGATAAAGAACTTCGATCAATGATTG
  AACAAGATGTCAAAAGAACGTTTCCTGAAATGCAGTTTTTCCAGCAAGAAAATGTGAGAAAAATTCTTAC
  AGATGTTCTTTTCTGTTATGCCAGAGAAAACGAGCAGTTGCTTTATAAACAGGGCATGCACGAACTGTTA
  GCACCTATAGTCTTTGTCCTTCACTGTGACCACCAAGCTTTTCTACATGCCAGTGAGTCTGCACAGCCCA
  GTGAGGAAATGAAAACTGTCTTGAACCCTGAGTATCTGGAACATGATGCCTATGCAGTGTTCTCACAACT
  TATGGAAACTGCTGAACCTTGGTTTTCAACTTTTGAGCATGATGGTCAGAAGGGGAAAGAAACACTGATG
  ACTCCCATTCCCTTTGCTAGACCACAAGATTTAGGGCCAACAATTGCTATTGTTACTAAAGTCAACCAGA
  TCCAGGATCATCTACTGAAGAAGCATGATATTGAGCTTTACATGCACTTGAACAGACTAGAAATTGCACC
  ACAGATATATGGGTTAAGGTGGGTGCGGCTGCTATTTGGACGAGAGTTCCCCCTGCAGGACCTTCTGGTG
  GTCTGGGATGCCTTGTTTGCAGACGGCCTCAGCCTGGGTTTAGTAGATTATATCTTCGTAGCCATGTTAC
  TTTACATCCGAGATGCTTTGATCTCTAGTAACTACCAGACCTGTCTCGGCCTTCTGATGCATTACCCATT
  CATCGGGGATGTACACTCACTGATTCTTAAGGCTCTGTTCCTTAGAGATCCAAAGAGAAATCCAAGACCA
  GTGACTTATCAATTCCATCCAAATTTAGATTATTACAAAGCACGAGGAGCAGACCTCATGAATAAAAGCC
  GGACCAATGCCAAAGGTGCTCCCCTGAATATAAATAAGGTCTCTAATAGCCTGATTAATTTTGGAAGAAA
  GTTGATTTCCCCAGCAATGGCTCCAGGCAGTGCAGGTGGCCCTGTACCTGGAGGCAACAGCAGTAGCTCC
  TCCTCTGTTGTAATTCCTACCAGGACCTCAGCAGAGGCCCCAAGCCATCACTTGCAACAGCAACAGCAGC
  AGCAGAGGCTGATGAAATCAGAAAGCATGCCTGTGCAATTGAACAAAGGGCTAAGTTCTAAAAACATCAG
  TTCATCTCCAAGCGTTGAGAGTTTGCCTGGAGGAAGAGAATTCACTGGCTCTCCACCTTCATCTGCTACT
  AAAAAAGATTCCTTTTTTAGCAACATCTCACGTTCTCGCTCACACAGCAAAACTATGGGCAGAAAAGAAT
  CTGAAGAAGAATTAGAAGCCCAAATTTCCTTCCTTCAAGGGCAGTTGAATGACCTGGATGCCATGTGCAA
  ATACTGTGCAAAGGTGATGGACACTCATCTTGTAAATATTCAAGATGTGATATTACAAGAAAATTTGGAA
  AAAGAAGATCAAATTCTGGTTTCCCTGGCAGGATTAAAACAGATCAAAGACATTCTAAAAGGTTCCCTGC
  GTTTTAACCAGAGCCAGCTAGAGGCCGAAGAGAACGAACAGATCACCATTGCGGACAACCACTACTGCTC
  CAGCGGCCAGGGCCAGGGCCGAGGCCAAGGCCAGAGCGTTCAAATGTCAGGGGCCATTAAACAGGCCTCT
  TCAGAAACGCCAGGGTGCACTGATAGAGGGAATTCCGATGACTTCATCCTGATTTCCAAAGATGATGATG
  GGAGCAGTGCCAGGGGCTCCTTCTCCGGCCAGGCCCAGCCTCTTCGCACCCTCAGAAGCACCTCTGGGAA
  AAGCCAGGCCCCAGTCTGCTCCCCACTGGTGTTCTCAGATCCACTGATGGGCCCAGCCTCAGCTTCCTCC
  AGCAACCCCAGCTCCAGTCCTGATGACGACAGCAGCAAGGACTCTGGCTTCACCATTGTGAGTCCCCTGG
  ACATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204401 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MYHSLSETRHPLQPEEQEVGIDPLSSYSNKSGGDSNKNGRRTSSTLDSEGTFNSYRKEWEELFVNNNYLA
  TIRQKGINGQLRSSRFRSICWKLFLCVLPQDKSQWISRIEELRAWYSNIKEIHITNPRKVVGQQDLMINN
  PLSQDEGSLWNKFFQDKELRSMIEQDVKRTFPEMQFFQQENVRKILTDVLFCYARENEQLLYKQGMHELL
  APIVFVLHCDHQAFLHASESAQPSEEMKTVLNPEYLEHDAYAVFSQLMETAEPWFSTFEHDGQKGKETLM
  TPIPFARPQDLGPTIAIVTKVNQIQDHLLKKHDIELYMHLNRLEIAPQIYGLRWVRLLFGREFPLQDLLV
  VWDALFADGLSLGLVDYIFVAMLLYIRDALISSNYQTCLGLLMHYPFIGDVHSLILKALFLRDPKRNPRP
  VTYQFHPNLDYYKARGADLMNKSRTNAKGAPLNINKVSNSLINFGRKLISPAMAPGSAGGPVPGGNSSSS
  SSVVIPTRTSAEAPSHHLQQQQQQQRLMKSESMPVQLNKGLSSKNISSSPSVESLPGGREFTGSPPSSAT
  KKDSFFSNISRSRSHSKTMGRKESEEELEAQISFLQGQLNDLDAMCKYCAKVMDTHLVNIQDVILQENLE
  KEDQILVSLAGLKQIKDILKGSLRFNQSQLEAEENEQITIADNHYCSSGQGQGRGQGQSVQMSGAIKQAS
  SETPGCTDRGNSDDFILISKDDDGSSARGSFSGQAQPLRTLRSTSGKSQAPVCSPLVFSDPLMGPASASS
  SNPSSSPDDDSSKDSGFTIVSPLDI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014744
• ORF Size: 2385 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014744.2
• RefSeq Size: 7869 bp
• RefSeq ORF: 2388 bp
• Locus ID: 9779
• UniProt ID: Q92609
• Cytogenetics: 3p24.3
• Domains: TBC
• MW: 89 kDa
• Gene Summary: May act as a GTPase-activating protein (GAP) for Rab family protein(s). May act as a GAP for RAB7A. Can displace RAB7A and retromer CSC subcomplex from the endosomal membrane to the cytosol; at least retromer displacement seems to require its catalytic activity (PubMed:19531583, PubMed:20923837). Required for retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN); the function seems to require its catalytic activity. Involved in regulation of autophagy (PubMed:22354992). May act as a molecular switch between endosomal and autophagosomal transport and is involved in reprogramming vesicle trafficking upon autophagy induction. Involved in the trafficking of ATG9A upon activation of autophagy. May regulate the recruitment of ATG9A-AP2-containing vesicles to autophagic membranes (PubMed:24603492).[UniProtKB/Swiss-Prot Function]