C12orf4 (NM_020374) Human Tagged ORF Clone

CAT#: RC204390

C12orf4 (Myc-DDK-tagged)-Human chromosome 12 open reading frame 4 (C12orf4)

Product Images

Circular map for RC204390

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C12orf4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204390 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAAAAACAGAGAAAGATTCTGCAATAGAGAGAGAGAATTTGTATATAAATTTAAAGTAGGAAGTC
  AGTGCTTAGAACTGAGAGTGCCACTCAAATTTCCTGTTCAAGAGAATGCCAGTCATTTGCATGGACGTCT
  GATGCTGCTGCACAGTTTACCGTGCTTTATAGAAAAAGACTTAAAAGAAGCTCTGACTCAGTTTATAGAA
  GAAGAATCCCTCAGCGATTATGATAGAGATGCTGAAGCATCCCTGGCAGCTGTGAAATCAGGTGAAGTAG
  ATTTACATCAGCTGGCGAGTACATGGGCCAAAGCTTATGCTGAGACCACGTTAGAGCATGCAAGGCCTGA
  AGAACCCAGCTGGGATGAAGATTTTGCAGATGTGTACCATGACTTAATTCATTCTCCTGCCTCTGAAACT
  CTCTTAAATTTGGAACATAATTACTTTGTTAGTATCTCAGAACTGATTGGTGAAAGAGATGTGGAGCTGA
  AAAAATTACGAGAGAGACAAGGTATTGAAATGGAAAAAGTCATGCAGGAATTGGGAAAATCACTGACAGA
  TCAAGATGTAAATTCACTGGCTGCTCAGCATTTTGAATCCCAGCAAGACCTAGAAAATAAATGGTCGAAT
  GAATTAAAACAATCAACTGCCATCCAAAAACAAGAGTATCAAGAATGGGTAATAAAACTTCACCAAGACC
  TAAAAAACCCCAACAACAGCTCCCTTAGTGAGGAAATTAAAGTTCAGCCAAGTCAGTTCAGAGAATCTGT
  AGAAGCAATTGGAAGGATTTATGAGGAACAGAGAAAGTTAGAAGAAAGTTTTACCATTCACTTAGGAGCC
  CAGTTGAAGACCATGCATAATTTGAGATTGCTGAGAGCAGATATGCTGGACTTCTGTAAGCATAAAAGAA
  ATCATCGAAGTGGTGTGAAACTTCATCGGCTCCAAACAGCTCTGTCACTTTATTCTACATCTCTCTGTGG
  CCTGGTTTTACTAGTAGATAATCGAATTAATTCATATAGTGGTATTAAAAGAGATTTTGCCACAGTTTGC
  CAAGAATGCACTGACTTCCATTTCCCCCGAATTGAAGAGCAATTAGAAGTTGTCCAACAGGTGGTACTTT
  ATGCTAGAACCCAGCGCAGGAGTAAATTGAAAGAATCACTTGATTCTGGAAACCAAAATGGAGGAAATGA
  TGATAAGACTAAGAATGCTGAGAGGAACTATTTAAATGTTTTACCTGGGGAATTTTATATTACACGGCAT
  TCTAATCTCTCAGAAATCCATGTTGCTTTCCATCTCTGTGTGGATGACCATGTGAAATCGGGAAACATCA
  CTGCTCGTGATCCTGCCATTATGGGACTCCGAAATATACTCAAAGTTTGCTGTACCCATGACATCACAAC
  AATAAGCATTCCTCTCTTGCTGGTACATGATATGTCAGAGGAAATGACTATACCCTGGTGCTTAAGGAGA
  GCGGAACTTGTGTTCAAGTGTGTCAAAGGTTTCATGATGGAAATGGCTTCATGGGATGGAGGAATTTCTA
  GGACAGTGCAATTTCTAGTACCACAGAGTATTTCTGAAGAAATGTTTTATCAACTTAGTAACATGCTTCC
  CCAGATCTTCCGAGTATCATCAACACTCACTCTGACATCCAAGCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204390 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKKNRERFCNREREFVYKFKVGSQCLELRVPLKFPVQENASHLHGRLMLLHSLPCFIEKDLKEALTQFIE
  EESLSDYDRDAEASLAAVKSGEVDLHQLASTWAKAYAETTLEHARPEEPSWDEDFADVYHDLIHSPASET
  LLNLEHNYFVSISELIGERDVELKKLRERQGIEMEKVMQELGKSLTDQDVNSLAAQHFESQQDLENKWSN
  ELKQSTAIQKQEYQEWVIKLHQDLKNPNNSSLSEEIKVQPSQFRESVEAIGRIYEEQRKLEESFTIHLGA
  QLKTMHNLRLLRADMLDFCKHKRNHRSGVKLHRLQTALSLYSTSLCGLVLLVDNRINSYSGIKRDFATVC
  QECTDFHFPRIEEQLEVVQQVVLYARTQRRSKLKESLDSGNQNGGNDDKTKNAERNYLNVLPGEFYITRH
  SNLSEIHVAFHLCVDDHVKSGNITARDPAIMGLRNILKVCCTHDITTISIPLLLVHDMSEEMTIPWCLRR
  AELVFKCVKGFMMEMASWDGGISRTVQFLVPQSISEEMFYQLSNMLPQIFRVSSTLTLTSKH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020374
• ORF Size: 1656 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020374.4
• RefSeq Size: 3830 bp
• RefSeq ORF: 1659 bp
• Locus ID: 57102
• UniProt ID: Q9NQ89
• Cytogenetics: 12p13.32
• MW: 63.8 kDa
• Gene Summary: This gene is highly conserved from nematodes to humans. In rat, the orthologous gene encodes a cytoplasmic protein that is involved in mast cell degranulation. The human gene has been implicated in autosomal recessive intellectual disability. [provided by RefSeq, Sep 2016]