C10orf84 (FAM204A) (NM_022063) Human Tagged ORF Clone

CAT#: RC204309

FAM204A (Myc-DDK-tagged)-Human chromosome 10 open reading frame 84 (C10orf84), transcript variant 1

Product Images

Circular map for RC204309

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C10orf84
• Synonyms: bA319I23.1; C10orf84
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204309 representing NM_022063
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAGTGGGCTGCTACCTCCTGGCCTAAATGAAAGTGACGCTGAGTCAAACTCGGAAGATGAAGCTA
  CGTTGGAGAACTCTGGACTTAACTTACAGGAAGATAAAGAGGATGAGAGCATCAGAAAAACAGAAATCAT
  AGATTTCTCAACAGATGAACCAAAAACTGAAACAGAGTCAAATGTAAATGCCTATGAAGAGTGTCCTTCT
  GGAATTCCCATAGATATGTGGAATAAATTTCAAGAATTGCATAAAAAACATTCTGAACAGAAAAGCACAA
  CCTCAAGATTCAGAGGGAAAAGAAGAAAACGCTCCAGAAAAGATAAATTGAAGAATGAAAAAGAATTACA
  TAGTGAACCGTCCTCAAATGAAACCCAGTGGAAAGAGCTTACTCAGTATTTTGGAGTCAATGATAGATTT
  GACCCGCCTGTTAAAAGGAAAAAAGTTGAGAAGTCAGGCCTTGAAAAGAGGATAGACCAGGCTGTGGAGG
  AGTGGAATATTGAGAAGGCTGAGGAACTCAGCAACCAGCTAGCTACTCGAGAGCTTGGTGTAAAAATTGC
  CAAAGCAGTTGCCTGCCACAACTTTGTAAAAGCCAAAAAGGAGGTTGAAAATTCACAGGCTGCCCGAAAA
  AAGAAGAAACTTGCATGGGGGTTTGAAGCAAAGAAGAGATGGGAAACCAAAAGCAACATGGGATACATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204309 representing NM_022063
  Red=Cloning site Green=Tags(s)
  
  MWSGLLPPGLNESDAESNSEDEATLENSGLNLQEDKEDESIRKTEIIDFSTDEPKTETESNVNAYEECPS
  GIPIDMWNKFQELHKKHSEQKSTTSRFRGKRRKRSRKDKLKNEKELHSEPSSNETQWKELTQYFGVNDRF
  DPPVKRKKVEKSGLEKRIDQAVEEWNIEKAEELSNQLATRELGVKIAKAVACHNFVKAKKEVENSQAARK
  KKKLAWGFEAKKRWETKSNMGYM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022063
• ORF Size: 699 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022063.3
• RefSeq Size: 2766 bp
• RefSeq ORF: 702 bp
• Locus ID: 63877
• UniProt ID: Q9H8W3
• Cytogenetics: 10q26.11
• MW: 27 kDa