DPP2 (DPP7) (NM_013379) Human Tagged ORF Clone

CAT#: RC204237

DPP7 (Myc-DDK-tagged)-Human dipeptidyl-peptidase 7 (DPP7)

Product Images

Circular map for RC204237

• 

  Western blot validation of overexpression lysate (Cat# LY402258) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204237 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified DPP7 protein (Cat# TP304237). The protein was produced from HEK293T cells transfected with DPP7 cDNA clone (Cat# RC204237) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DPP2
• Synonyms: DPP2; DPPII; QPP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204237 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTCCGCTCCCTGGGCCCCGGTCCTGCTGCTGGCGCTCGGGCTGCGCGGCCTCCAGGCGGGGGCCC
  GCAGGGCCCCGGACCCCGGCTTCCAGGAGCGCTTCTTCCAGCAGCGTCTGGACCACTTCAACTTCGAGCG
  CTTCGGCAACAAGACCTTCCCCCAGCGCTTCCTGGTGTCGGACAGGTTCTGGGTCCGGGGCGAGGGGCCC
  ATCTTCTTCTACACTGGGAACGAGGGCGACGTGTGGGCCTTCGCCAACAACTCGGGCTTCGTCGCGGAGC
  TGGCGGCCGAGCGGGGGGCTCTACTGGTCTTCGCGGAGCACCGCTACTACGGGAAGTCGCTGCCGTTCGG
  TGCGCAGTCCACGCAGCGCGGGCACACGGAGCTGCTGACGGTGGAGCAGGCCCTGGCCGACTTCGCAGAG
  CTGCTCCGCGCGCTACGACGCGACCTCGGGGCCCAGGATGCCCCCGCCATCGCCTTCGGTGGAAGTTATG
  GGGGGATGCTCAGTGCCTACCTGAGGATGAAGTATCCCCACCTGGTGGCGGGGGCGCTGGCGGCCAGCGC
  GCCCGTTCTAGCTGTGGCAGGCCTCGGCGACTCCAACCAGTTCTTCCGGGACGTCACGGCGGACTTTGAG
  GGCCAGAGTCCCAAATGCACCCAGGGTGTGCGGGAAGCGTTCCGACAGATCAAGGACTTGTTCCTACAGG
  GAGCCTACGACACGGTCCGCTGGGAGTTCGGCACCTGCCAGCCGCTGTCAGACGAGAAGGACCTGACCCA
  GCTCTTCATGTTCGCCCGGAATGCCTTCACCGTGCTGGCCATGATGGACTACCCCTACCCCACTGACTTC
  CTGGGTCCCCTCCCTGCCAACCCCGTCAAGGTGGGCTGTGATCGGCTGCTGAGTGAGGCCCAGAGGATCA
  CGGGGCTGCGAGCACTGGCAGGGCTGGTCTACAACGCCTCGGGCTCCGAGCACTGCTACGACATCTACCG
  GCTCTACCACAGCTGTGCTGACCCCACTGGCTGCGGCACCGGCCCCGACGCCAGGGCCTGGGACTACCAG
  GCCTGCACCGAGATCAACCTGACCTTCGCCAGCAACAATGTGACCGATATGTTCCCCGACCTGCCCTTCA
  CTGACGAGCTCCGCCAGCGGTACTGCCTGGACACCTGGGGCGTGTGGCCCCGGCCCGACTGGCTGCTGAC
  CAGCTTCTGGGGGGGTGATCTCAGAGCCGCCAGCAACATCATCTTCTCCAACGGGAACCTGGACCCCTGG
  GCAGGGGGCGGGATTCGGAGGAACCTGAGTGCCTCAGTCATCGCCGTCACCATCCAGGGGGGAGCGCACC
  ACCTCGACCTCAGAGCCTCCCACCCAGAAGATCCTGCTTCCGTGGTTGAGGCGCGGAAGCTGGAGGCCAC
  CATCATCGGCGAGTGGGTAAAGGCAGCCAGGCGTGAGCAGCAGCCAGCTCTGCGTGGGGGGCCCAGACTC
  AGCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204237 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGSAPWAPVLLLALGLRGLQAGARRAPDPGFQERFFQQRLDHFNFERFGNKTFPQRFLVSDRFWVRGEGP
  IFFYTGNEGDVWAFANNSGFVAELAAERGALLVFAEHRYYGKSLPFGAQSTQRGHTELLTVEQALADFAE
  LLRALRRDLGAQDAPAIAFGGSYGGMLSAYLRMKYPHLVAGALAASAPVLAVAGLGDSNQFFRDVTADFE
  GQSPKCTQGVREAFRQIKDLFLQGAYDTVRWEFGTCQPLSDEKDLTQLFMFARNAFTVLAMMDYPYPTDF
  LGPLPANPVKVGCDRLLSEAQRITGLRALAGLVYNASGSEHCYDIYRLYHSCADPTGCGTGPDARAWDYQ
  ACTEINLTFASNNVTDMFPDLPFTDELRQRYCLDTWGVWPRPDWLLTSFWGGDLRAASNIIFSNGNLDPW
  AGGGIRRNLSASVIAVTIQGGAHHLDLRASHPEDPASVVEARKLEATIIGEWVKAARREQQPALRGGPRL
  SL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013379
• ORF Size: 1476 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013379.3
• RefSeq Size: 1633 bp
• RefSeq ORF: 1479 bp
• Locus ID: 29952
• UniProt ID: Q9UHL4
• Cytogenetics: 9q34.3
• Domains: abhydrolase
• Protein Families: Protease
• MW: 54.3 kDa
• Gene Summary: The protein encoded by this gene is a post-proline cleaving aminopeptidase expressed in quiescent lymphocytes. The resting lymphocytes are maintained through suppression of apoptosis, a state which is disrupted by inhibition of this novel serine protease. The enzyme has strong sequence homology with prolylcarboxypeptidase and is active at both acidic and neutral pH. [provided by RefSeq, Jul 2008]