Syntaxin 5A (STX5) (NM_003164) Human Tagged ORF Clone

CAT#: RC204213

STX5 (Myc-DDK-tagged)-Human syntaxin 5 (STX5)

Product Images

Circular map for RC204213

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  Western blot validation of overexpression lysate (Cat# LY418853) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204213 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified STX5 protein (Cat# TP304213). The protein was produced from HEK293T cells transfected with STX5 cDNA clone (Cat# RC204213) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Syntaxin 5A
• Synonyms: SED5; STX5A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204213 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCCCGCGGAAACGCTACGGGTCTAAGAACACGGATCAGGGTGTCTACCTGGGTCTCTCAAAGACAC
  AGGTCCTGTCCCCTGCAACTGCTGGCAGTAGCAGCAGCGACATCGCCCCTCTGCCCCCCCCAGTGACCCT
  CGTCCCTCCCCCTCCCGACACCATGTCCTGCCGGGATCGGACCCAGGAGTTTCTGTCTGCCTGCAAGTCG
  CTGCAGACCCGTCAGAATGGAATCCAGACAAATAAGCCAGCTTTGCGTGCTGTCCGACAACGCAGTGAAT
  TCACCCTCATGGCCAAGCGCATTGGGAAAGACCTTAGCAACACATTTGCCAAGCTGGAGAAGCTGACAAT
  CTTGGCAAAGCGCAAGTCCCTCTTTGATGATAAAGCAGTGGAAATTGAAGAGCTAACATATATCATCAAA
  CAGGACATCAATAGCCTCAACAAACAAATTGCTCAGCTCCAGGATTTCGTGAGAGCCAAGGGCAGCCAGA
  GTGGCCGGCACCTGCAGACCCACTCCAACACCATTGTGGTCTCCTTGCAGTCGAAACTGGCTTCTATGTC
  CAATGACTTCAAATCGGTTTTAGAAGTGAGGACAGAGAACCTGAAGCAGCAGAGGAGCCGGAGAGAGCAG
  TTCTCCCGGGCACCTGTGTCAGCCCTGCCCCTTGCCCCTAACCACCTGGGCGGTGGTGCTGTGGTTCTGG
  GGGCAGAGTCCCATGCCTCCAAGGATGTCGCCATCGACATGATGGACTCTCGGACCAGCCAGCAGCTGCA
  GCTCATTGACGAGCAGGATTCCTACATCCAGAGTCGGGCAGACACCATGCAGAACATTGAGTCGACAATT
  GTTGAGTTGGGCTCCATCTTTCAGCAGTTGGCACACATGGTTAAGGAACAGGAGGAAACCATTCAGAGGA
  TCGACGAGAACGTGCTAGGAGCCCAGCTGGACGTTGAGGCCGCCCATTCAGAGATCCTCAAGTACTTCCA
  GTCTGTCACCTCCAACCGGTGGCTCATGGTCAAAATCTTCCTCATCCTCATTGTCTTCTTCATCATCTTT
  GTGGTCTTCCTTGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204213 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MIPRKRYGSKNTDQGVYLGLSKTQVLSPATAGSSSSDIAPLPPPVTLVPPPPDTMSCRDRTQEFLSACKS
  LQTRQNGIQTNKPALRAVRQRSEFTLMAKRIGKDLSNTFAKLEKLTILAKRKSLFDDKAVEIEELTYIIK
  QDINSLNKQIAQLQDFVRAKGSQSGRHLQTHSNTIVVSLQSKLASMSNDFKSVLEVRTENLKQQRSRREQ
  FSRAPVSALPLAPNHLGGGAVVLGAESHASKDVAIDMMDSRTSQQLQLIDEQDSYIQSRADTMQNIESTI
  VELGSIFQQLAHMVKEQEETIQRIDENVLGAQLDVEAAHSEILKYFQSVTSNRWLMVKIFLILIVFFIIF
  VVFLA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003164
• ORF Size: 1065 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003164.5
• RefSeq Size: 1834 bp
• RefSeq ORF: 1068 bp
• Locus ID: 6811
• UniProt ID: Q13190
• Cytogenetics: 11q12.3
• Domains: t_SNARE, SynN
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: SNARE interactions in vesicular transport
• MW: 39.7 kDa
• Gene Summary: This gene encodes a member of the syntaxin or t-SNARE (target-SNAP receptor) family. These proteins are found on cell membranes and serve as the targets for v-SNAREs (vesicle-SNAP receptors), permitting specific synaptic vesicle docking and fusion. The encoded protein regulates endoplasmic reticulum to Golgi transport and plays a critical role in autophagy. Autoantibodies targeting the encoded protein may be a diagnostic marker for endometriosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]