Islet 2 (ISL2) (NM_145805) Human Tagged ORF Clone

CAT#: RC204212

ISL2 (Myc-DDK-tagged)-Human ISL LIM homeobox 2 (ISL2)

Product Images

Circular map for RC204212

• 

  Western blot validation of overexpression lysate (Cat# LY407857) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204212 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Islet 2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204212 representing NM_145805
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGATATTATTTTTCATTATCCTTTTCTGGGTGCTATGGGTGATCATTCCAAGAAGAAGCCCGGGA
  CGGCCATGTGCGTGGGCTGCGGGAGTCAGATCCACGACCAGTTTATCCTGCGGGTGTCGCCCGACCTCGA
  GTGGCACGCGGCCTGCCTCAAGTGTGCCGAGTGCAGCCAGTACCTGGACGAGACGTGCACGTGCTTCGTG
  AGAGACGGGAAGACCTACTGCAAGCGGGACTACGTCAGGCTGTTCGGCATCAAGTGCGCCAAGTGCCAGG
  TGGGCTTCAGCAGCAGCGACCTGGTGATGAGGGCGCGGGACAGCGTGTACCACATCGAGTGCTTCCGCTG
  CTCCGTGTGCAGCCGCCAGCTGCTGCCTGGGGACGAGTTCTCGCTGCGGGAGCACGAGCTGCTCTGCCGC
  GCCGACCACGGCCTCCTGCTCGAGCGCGCCGCGGCCGGCAGCCCGCGCAGCCCCGGCCCGCTTCCCGGCG
  CCCGCGGCCTGCATCTGCCCGACGCTGGGTCGGGCCGGCAGCCCGCGTTGCGCCCGCACGTGCACAAGCA
  GACGGAGAAGACGACCCGCGTGCGGACTGTGCTGAACGAGAAGCAGCTGCACACTCTGCGGACCTGCTAC
  GCCGCCAACCCGCGGCCCGACGCTCTCATGAAGGAGCAGCTGGTGGAGATGACCGGCCTGAGCCCGCGGG
  TCATCCGCGTCTGGTTCCAGAACAAGCGCTGCAAGGACAAGAAGAAATCCATTCTCATGAAGCAGCTGCA
  GCAGCAGCAGCACAGCGACAAGACGAGCCTTCAGGGACTGACTGGGACGCCCCTGGTGGCGGGCAGTCCC
  ATCCGCCATGAGAACGCCGTGCAGGGCAGCGCAGTGGAGGTGCAGACGTACCAGCCGCCGTGGAAGGCGC
  TCAGCGAGTTTGCCCTCCAGAGCGACCTGGACCAACCCGCCTTCCAACAGCTGGTCTCCTTCTCCGAGTC
  CGGCTCCCTAGGCAACTCCTCCGGCAGCGACGTGACCTCCCTGTCCTCGCAGCTCCCGGACACCCCCAAC
  AGTATGGTGCCGAGTCCCGTGGAGACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204212 representing NM_145805
  Red=Cloning site Green=Tags(s)
  
  MVDIIFHYPFLGAMGDHSKKKPGTAMCVGCGSQIHDQFILRVSPDLEWHAACLKCAECSQYLDETCTCFV
  RDGKTYCKRDYVRLFGIKCAKCQVGFSSSDLVMRARDSVYHIECFRCSVCSRQLLPGDEFSLREHELLCR
  ADHGLLLERAAAGSPRSPGPLPGARGLHLPDAGSGRQPALRPHVHKQTEKTTRVRTVLNEKQLHTLRTCY
  AANPRPDALMKEQLVEMTGLSPRVIRVWFQNKRCKDKKKSILMKQLQQQQHSDKTSLQGLTGTPLVAGSP
  IRHENAVQGSAVEVQTYQPPWKALSEFALQSDLDQPAFQQLVSFSESGSLGNSSGSDVTSLSSQLPDTPN
  SMVPSPVET
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145805
• ORF Size: 1077 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145805.1, NP_665804.1
• RefSeq Size: 1836 bp
• RefSeq ORF: 1080 bp
• Locus ID: 64843
• UniProt ID: Q96A47
• Cytogenetics: 15q24.3
• Protein Families: Transcription Factors
• MW: 39.6 kDa
• Gene Summary: Transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways.[UniProtKB/Swiss-Prot Function]